DOI: 10.1111/j.1432-1033.1973.tb02692.x. Fractionation of Membrane‐Bound Polysomes, Free Polysomes, and Nuclei from Tissue‐Cultured Cells

Fractionation of Membrane‐Bound Polysomes, Free Polysomes, and Nuclei from Tissue‐Cultured Cells

10.1111/j.1432-1033.1973.tb02692.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Free polysomes from many kinds of eukaryotic cells are liberated by cell lysis using the non‐ionic detergent Kyro EOB leaving a residue of nuclei with strands of rough endoplasmic reticulum attached. Subsequent treatment with a combination of Kyro EOB plus deoxycholate solubilizes the membrane‐bound polysomes. Polysomes prepared in this manner function well in amino‐acid‐incorporating systems in vitro. The nuclei remaining incorporate [14C]ATP into RNA. More active nuclei, however, are obtained when cells are disrupted by nitrogen cavitation.

Bibliography

Birckbichler, P. J., & Pryme, I. F. (1973). Fractionation of MembraneâBound Polysomes, Free Polysomes, and Nuclei from TissueâCultured Cells. European Journal of Biochemistry, 33(2), 368â373. Portico.

Authors 2

Paul J. Birckbichler (first)
Ian F. Pryme (additional)

References 21 Referenced 43

10.1083/jcb.2.2.171
10.1083/jcb.22.1.227
10.1016/S0021-9258(18)94321-8
10.1073/pnas.63.4.1370
10.1126/science.164.3879.584
10.1042/bj1030177
10.1016/0022-2836(67)90297-5
10.1016/0005-2787(72)90035-4
10.1126/science.130.3373.432
{'key': 'e_1_2_2_11_2', 'first-page': '405', 'volume': '36', 'author': 'Moore G. E.', 'year': '1966', 'journal-title': 'J. Nat. Cancer Inst.'} / J. Nat. Cancer Inst. by Moore G. E. (1966)
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10.1016/0014-4827(69)90128-1
10.1016/0014-5793(68)80083-3
10.1021/bi00849a036
10.1016/S0076-6879(67)12035-1
10.1016/S0021-9258(18)62646-8 / J. Biol. Chem. by Venrooij W. J. W. (1970)
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{'key': 'e_1_2_2_19_2', 'first-page': '173', 'volume': '222', 'author': 'Davis B. K.', 'year': '1969', 'journal-title': 'Nature (London)'} / Nature (London) by Davis B. K. (1969)
10.1016/0005-2787(71)90593-4
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10.1016/S0022-2836(68)80035-X

Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 6:02 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 10:18 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 11:07 a.m.)
Issued	52 years, 5 months ago (March 1, 1973)
Published	52 years, 5 months ago (March 1, 1973)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	52 years, 5 months ago (March 1, 1973)

Funders 0

None

BibTeX

@article{Birckbichler_1973, title={Fractionation of Membrane‐Bound Polysomes, Free Polysomes, and Nuclei from Tissue‐Cultured Cells}, volume={33}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1973.tb02692.x}, DOI={10.1111/j.1432-1033.1973.tb02692.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Birckbichler, Paul J. and Pryme, Ian F.}, year={1973}, month=mar, pages={368–373} }

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