DOI: 10.1111/j.1432-1033.1973.tb02673.x. Dissociation of Single Ribosomes as a Preliminary Step for Their Participation in Protein Synthesis

Dissociation of Single Ribosomes as a Preliminary Step for Their Participation in Protein Synthesis

10.1111/j.1432-1033.1973.tb02673.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

32P‐labelled derived 40‐S ribosomal subunits and 80‐S monoribosomes entered the polyribosome fraction of a rabbit‐reticulocyte lysate active in protein synthesis by a process that was dependent on time, energy and temperature. Both 40‐S and 60‐S subparticles from the labelled monoribosome entered the polyribosomes at the same rate. The addition of excess unlabelled 40‐S subparticles to the cell‐free system containing labelled monoribosomes increased the ratio of 60‐S to 40‐S‐subparticle radioactivity in the polyribosome fraction. This indicated a competition between added unlabelled and monoribosome‐derived 40‐S subparticles for entry into polyribosomes. From the evaluation of the translation time of mRNA, of the rate of monoribosome‐polyribosome exchange, and of the equilibrium distribution of the cycling labelled monoribosomes and unlabelled subparticles with polyribosomes it is concluded that the dissociation of single ribosomes into subunits is an obligatory step for their entry into the polyribosoma1 fraction and that this process is coupled with initiation of mRNA translation.

Bibliography

Ottolenghi, S., Comi, P., Giglioni, B., Gianni, A. M., & Guidotti, G. G. (1973). Dissociation of Single Ribosomes as a Preliminary Step for Their Participation in Protein Synthesis. European Journal of Biochemistry, 33(2), 227â232. Portico.

Authors 5

Sergio Ottolenghi (first)
Paola Comi (additional)
Barbara Giglioni (additional)
Alessandro M. Gianni (additional)
Guido G. Guidotti (additional)

References 26 Referenced 7

10.1073/pnas.63.4.1206
10.1021/bi00837a038
10.1073/pnas.61.2.651
10.1016/0005-2787(71)90058-X
10.1016/0005-2787(70)90630-1
10.1016/S0021-9258(18)62684-5
10.1111/j.1432-1033.1971.tb19666.x
{'key': 'e_1_2_1_9_2', 'first-page': '143', 'volume': '20', 'author': 'Comi P.', 'year': '1971', 'journal-title': 'Ital. J. Biochem.'} / Ital. J. Biochem. by Comi P. (1971)
10.1016/0022-2836(70)90043-4
10.1016/0006-291X(65)90159-2
10.1016/0005-2787(66)90379-0
10.1016/0005-2787(68)90014-2
{'key': 'e_1_2_1_14_2', 'first-page': '397', 'volume': '19', 'author': 'Borghetti A. F.', 'year': '1970', 'journal-title': 'Ital. J. Biochem.'} / Ital. J. Biochem. by Borghetti A. F. (1970)
10.1016/S0300-9084(71)80073-1
{'key': 'e_1_2_1_16_2', 'first-page': '481', 'volume-title': 'Methods Enzymol.', 'author': 'Rich A.', 'year': '1968'} / Methods Enzymol. by Rich A. (1968)
10.1016/S0022-2836(64)80227-8
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10.1016/0022-2836(67)90047-2
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10.1073/pnas.61.2.719
10.1016/0005-2787(66)90045-1
10.1073/pnas.69.2.353

Dates

Type	When
Created	20 years, 6 months ago (March 4, 2005, 6:02 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 10:18 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 11:08 a.m.)
Issued	52 years, 6 months ago (March 1, 1973)
Published	52 years, 6 months ago (March 1, 1973)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	52 years, 6 months ago (March 1, 1973)

Funders 0

None

BibTeX

@article{Ottolenghi_1973, title={Dissociation of Single Ribosomes as a Preliminary Step for Their Participation in Protein Synthesis}, volume={33}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1973.tb02673.x}, DOI={10.1111/j.1432-1033.1973.tb02673.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Ottolenghi, Sergio and Comi, Paola and Giglioni, Barbara and Gianni, Alessandro M. and Guidotti, Guido G.}, year={1973}, month=mar, pages={227–232} }

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