DOI: 10.1111/j.1432-1033.1973.tb02663.x. Yeast Chromatin

Yeast Chromatin

10.1111/j.1432-1033.1973.tb02663.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

A chromatin‐like material was prepared from isolated nuclei of Saccharomyces cerevisiae. This chromatin fraction contains a full complement of yeast histones as revealed by acrylamide‐gel electrophoresis. Yeast histones were found to resemble those of rat liver cells in electrophoretic properties except for the almost complete absence of histone F1. Yeast chromatin is only poorly transcribed by the isolated yeast RNA polymerases A and B and was found to contain endogenous RNA polymerases which are activated by the addition of salts. Salt causes a removal of DNA‐associated proteins from the chromatin, allowing bound RNA polymerase molecules to extend already initiated RNA chains. Some evidence was obtained indicating that different proteins have to be removed to allow the different endogenous polymerases to proceed with transcription. It is concluded that the properties of yeast chromatin studied, in particular the histone composition and the transcription capacity, are very similar to those of chromatin preparations from animal cells.

Bibliography

Wintersberger, U., Letnansky, K., & Smith, P. (1973). Yeast Chromatin. European Journal of Biochemistry, 33(1), 123â130. Portico.

Authors 3

Ulrike Wintersberger (first)
Karl Letnansky (additional)
Phillip Smith (additional)

References 36 Referenced 83

{'key': 'e_1_2_2_2_2', 'first-page': '3', 'volume-title': 'Handbook of Molecular Cytology', 'author': 'McCarthy B. J.', 'year': '1969'} / Handbook of Molecular Cytology by McCarthy B. J. (1969)
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Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 10:01 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 10:06 a.m.)
Indexed	1 year, 7 months ago (Jan. 10, 2024, 12:23 a.m.)
Issued	52 years, 7 months ago (Feb. 1, 1973)
Published	52 years, 7 months ago (Feb. 1, 1973)
Published Online	20 years, 5 months ago (March 4, 2005)
Published Print	52 years, 7 months ago (Feb. 1, 1973)

Funders 0

None

BibTeX

@article{Wintersberger_1973, title={Yeast Chromatin: Preparation from Isolated Nuclei, Histone Composition and Transcription Capacity}, volume={33}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1973.tb02663.x}, DOI={10.1111/j.1432-1033.1973.tb02663.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Wintersberger, Ulrike and Letnansky, Karl and Smith, Phillip}, year={1973}, month=feb, pages={123–130} }

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