DOI: 10.1111/j.1432-1033.1973.tb02626.x. Purification and Properties of an Inhibitor of the Tryptophan‐Synthase‐Inactivating Enzymes in Yeast

Purification and Properties of an Inhibitor of the Tryptophan‐Synthase‐Inactivating Enzymes in Yeast

10.1111/j.1432-1033.1973.tb02626.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

An inhibitor of the tryptophan‐synthase‐inactivating enzymes has been isolated from bakers' yeast and purified 27‐fold on a protein basis, by heat treatment, precipitation by trichloroacetic acid, ethanol fractionation and acid precipitation. The inhibitory activity of the purified material is not affected by incubation with RNAase, DNAase, or muramidase, but is destroyed by incubation with pronase or trypsin. Inhibitory activity depends, therefore, on the protein content of the material.The extent to which the inactivating enzymes are inhibited is proportional to inhibitor concentration. Tryptophan‐synthase inactivases I and II are both inhibited. The inhibitor appears to be specific in its action: it is much more effective than soybean trypsin inhibitor in inhibiting the inactivases, but has comparatively little effect on the esterolytic activity of trypsin.Inhibitory activity was measured in crude extracts of Saccharomyces cerevisiae growing in batch culture. In the exponential growth phase, the inhibitor was barely detectable, but its levels subsequently rose at the end of the exponential phase and in the stationary phase. The possible biological significance of these observations is discussed.

Bibliography

Ferguson, A. R., Katsunuma, T., Betz, H., & Holzer, H. (1973). Purification and Properties of an Inhibitor of the TryptophanâSynthaseâInactivating Enzymes in Yeast. European Journal of Biochemistry, 32(3), 444â450. Portico.

Authors 4

A. Ross Ferguson (first)
Tsunehiko Katsunuma (additional)
Heinrich Betz (additional)
Helmut Holzer (additional)

References 14 Referenced 34

10.1111/j.1432-1033.1972.tb01868.x
10.1128/jb.96.2.403-408.1968 / J. Bacteriol. by Manney T. R. (1968)
10.1016/S0021-9258(19)52451-6
Schött H. E.(1973)Doctoral Dissertation University of Freiburg Germany .
{'key': 'e_1_2_3_6_2', 'first-page': '1021', 'volume-title': 'Methoden der Enzymatischen Analyse', 'author': 'Fritz H.', 'year': '1970'} / Methoden der Enzymatischen Analyse by Fritz H. (1970)
10.1271/bbb1961.31.150
10.1271/bbb1961.35.658
10.1042/bj1170843
10.1016/S0021-9258(18)65206-8 / J. Biol. Chem. by Lenney J. F. (1956)
10.1016/0003-9861(69)90194-5
10.1016/0006-291X(71)90051-9
10.1016/S0006-291X(72)80016-0 / Biochem. Biophys. Res. Commun. by Afting E. Q. (1971)
{'key': 'e_1_2_3_14_2', 'first-page': '59', 'volume': '35', 'author': 'Matile Ph.', 'year': '1969', 'journal-title': 'Antonie van Leeuwenhoek J. Microbiol. Serol.'} / Antonie van Leeuwenhoek J. Microbiol. Serol. by Matile Ph. (1969)
Hasilik A.(1973)Doctoral Dissertation University of Freiburg Germany .

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 10:15 a.m.)
Deposited	1 year, 10 months ago (Oct. 1, 2023, 7:41 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 5:55 p.m.)
Issued	52 years, 6 months ago (Feb. 1, 1973)
Published	52 years, 6 months ago (Feb. 1, 1973)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	52 years, 6 months ago (Feb. 1, 1973)

Funders 0

None

BibTeX

@article{Ferguson_1973, title={Purification and Properties of an Inhibitor of the Tryptophan‐Synthase‐Inactivating Enzymes in Yeast}, volume={32}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1973.tb02626.x}, DOI={10.1111/j.1432-1033.1973.tb02626.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Ferguson, A. Ross and Katsunuma, Tsunehiko and Betz, Heinrich and Holzer, Helmut}, year={1973}, month=feb, pages={444–450} }

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