DOI: 10.1111/j.1432-1033.1972.tb19710.x. Fate of Protein‐Containing Liposomes Injected into Rats

Fate of Protein‐Containing Liposomes Injected into Rats

10.1111/j.1432-1033.1972.tb19710.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

[3H]Amyloglucosidase and 131I‐labelled albumin were entrapped into liposomes (lipid spherules) composed of phosphatidyl choline, cholesterol and dicetyl phosphate (7:2:1 molar ratio). 131I‐labelled albumin was also entrapped in [3H]cholesterol‐liposomes.Investigations on the fate in vivo following intravenous injection of such protein‐containing liposomes into rats, showed that the bulk of liposomal radioactivity is removed from the blood within minutes. There is no measurable leakage of entrapped 131I‐labelled albumin while liposomes are in the circulation.Most of the removed liposomal radioactivity was recovered in the liver. Autoradiographic examination of the liver 3 min after injection of albumin‐containing [3H]cholesterol‐liposomes suggests that hepatocytes and probably Kupffer cells are involved in the uptake of liposomes. It appears that liposomes enter this tissue intact but subsequently liposomal membranes and entrapped protein are catabolized.These catabolic processes are probably occurring in the liver lysosomes, as most of the liposomal radioactivity in this tissue was recovered in the mitochondrial‐lysosomal fraction. Liposomes appear to be promising as a vehicle for the administration of enzymes and possibly also drugs to patients with storage diseases.

Bibliography

Gregoriadis, G., & Ryman, B. E. (1972). Fate of ProteinâContaining Liposomes Injected into Rats. European Journal of Biochemistry, 24(3), 485â491. Portico.

Authors 2

Gregory Gregoriadis (first)
Brenda E. Ryman (additional)

References 19 Referenced 261

10.1016/0014-5793(71)80109-6
10.1016/0079-6107(68)90019-9 / Progr. Biophys. by Bangham A. D. (1968)
10.1016/S0022-2275(20)43097-4
{'key': 'e_1_2_2_5_2', 'first-page': '507', 'volume-title': 'Immunochemical Techniques for the Identification and Estimation of Macromolecules', 'author': 'Clausen J.', 'year': '1969'} / Immunochemical Techniques for the Identification and Estimation of Macromolecules by Clausen J. (1969)
10.1021/bi00846a023
10.1139/o67-217
10.1042/bj0780106 / Biochem. J. by Borooah J. (1901)
10.1042/bj0800649
10.1016/0003-2697(69)90308-X
10.1016/S0021-9258(18)99337-3
10.1021/ac60226a017
10.1016/S0021-9258(18)62728-0
10.1111/j.1365-2818.1964.tb00541.x
10.1016/S0016-5085(65)80041-5 / Gastroenterology by Hers H. G. (1964)
10.1126/science.165.3894.698
10.1016/S0022-3476(70)80172-X
{'key': 'e_1_2_2_18_2', 'first-page': '259', 'volume': '90', 'author': 'Schulman J. D.', 'year': '1970', 'journal-title': 'Arch. Pathol.'} / Arch. Pathol. by Schulman J. D. (1970)
10.1172/JCI105417
{'first-page': '3', 'volume-title': 'Lysosomes in Biology and Pathology', 'author': 'Duve C.', 'key': 'e_1_2_2_20_2'} / Lysosomes in Biology and Pathology by Duve C.

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 9:34 a.m.)
Deposited	1 year, 10 months ago (Oct. 7, 2023, 9:21 a.m.)
Indexed	1 day, 16 hours ago (Sept. 4, 2025, 9:42 a.m.)
Issued	53 years, 8 months ago (Jan. 1, 1972)
Published	53 years, 8 months ago (Jan. 1, 1972)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	53 years, 8 months ago (Jan. 1, 1972)

Funders 0

None

BibTeX

@article{Gregoriadis_1972, title={Fate of Protein‐Containing Liposomes Injected into Rats: An Approach to the Treatment of Storage Diseases}, volume={24}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1972.tb19710.x}, DOI={10.1111/j.1432-1033.1972.tb19710.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Gregoriadis, Gregory and Ryman, Brenda E.}, year={1972}, month=jan, pages={485–491} }

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