DOI: 10.1111/j.1432-1033.1972.tb02112.x. Inhibition of the Assembly of Cytochrome Oxidase in <i>Neurospora crassa</i> by Chloramphenicol

Inhibition of the Assembly of Cytochrome Oxidase in Neurospora crassa by Chloramphenicol

10.1111/j.1432-1033.1972.tb02112.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Cytochrome oxidase was prepared from Neurospora crassa by chromatography on oleyl poly‐methacrylic acid resin and separated into seven polypeptides by polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate. Incorporation of labelled amino acids into the single polypeptides was investigated after a pulse labelling in the absence and presence of chloramphenicol, and after washing out the inhibitor.Chloramphenicol (4 mg/ml) inhibited amino acid incorporation into all polypeptides 90–95% while labelling of the whole membrane protein was inhibited only 30%. After washing out the inhibitor and further growth of the cells, the four smaller polypeptides were highly labelled, whereas the other polypeptides showed only a small increase in radioactivity. It is concluded that the four small‐sized polypeptides of cytochrome oxidase are synthesized but not integrated into the functional enzyme under the action of chloramphenicol.

Bibliography

Sebald, W., Weiss, H., & Jackl, G. (1972). Inhibition of the Assembly of Cytochrome Oxidase in Neurospora crassa by Chloramphenicol. European Journal of Biochemistry, 30(3), 413â417. Portico.

Authors 3

Walter Sebald (first)
Hanns Weiss (additional)
Gerhard Jackl (additional)

References 19 Referenced 83

10.1111/j.1432-1033.1971.tb01509.x / Eur. J. Bio chem. by Weiss H. (1971)
10.1016/0006-3002(60)91207-5
10.1016/0014-4827(59)90094-1
10.1016/0006-291X(66)90631-0
{'key': 'e_1_2_2_6_2', 'first-page': '42', 'volume': '13', 'author': 'Vogel H. J.', 'year': '1956', 'journal-title': 'Microbiol. Genet. Bull.'} / Microbiol. Genet. Bull. by Vogel H. J. (1956)
Schwab A. J. Sebald W.&Weiss H.(1972)FEBS Lett. in press.
10.1016/0005-2736(69)90042-X
10.1007/BF02097465
10.1016/S0021-9258(18)93274-6
10.1016/0014-5793(68)80071-7
{'key': 'e_1_2_2_12_2', 'first-page': '728', 'volume-title': '20. Mosbacher Colloquium', 'author': 'Sebald W.', 'year': '1969'} / 20. Mosbacher Colloquium by Sebald W. (1969)
10.1038/222142a0
10.1083/jcb.34.1.1
10.1007/BF00596956
10.1073/pnas.39.7.606
10.1016/S0021-9258(18)94305-X / J. Biol. Chem. by Tzagaloff A. (1969)
10.1083/jcb.51.1.249
10.1016/S0926-6593(66)80142-X
{'key': 'e_1_2_2_20_2', 'first-page': '1401', 'volume': '31', 'author': 'Mason T.', 'year': '1972', 'journal-title': 'Fed. Proc.'} / Fed. Proc. by Mason T. (1972)

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 10:03 a.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 5:39 p.m.)
Indexed	1 year, 3 months ago (May 12, 2024, 3:59 p.m.)
Issued	52 years, 9 months ago (Nov. 1, 1972)
Published	52 years, 9 months ago (Nov. 1, 1972)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	52 years, 9 months ago (Nov. 1, 1972)

Funders 0

None

BibTeX

@article{Sebald_1972, title={Inhibition of the Assembly of Cytochrome Oxidase in Neurospora crassa by Chloramphenicol}, volume={30}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1972.tb02112.x}, DOI={10.1111/j.1432-1033.1972.tb02112.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Sebald, Walter and Weiss, Hanns and Jackl, Gerhard}, year={1972}, month=nov, pages={413–417} }

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