DOI: 10.1111/j.1432-1033.1972.tb02109.x. Characterization of the Native and Denatured Conformations of tRNA<sup>Ser</sup>and tRNA<sup>Phe</sup>from Yeast

Characterization of the Native and Denatured Conformations of tRNASerand tRNAPhefrom Yeast

10.1111/j.1432-1033.1972.tb02109.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Previous work on the partial digestion of the native and denatured forms of tRNASerand tRNAPhefrom yeast with T1‐RNAase was extended. Detailed studies with pancreatic and T2‐RNAase and a few experiments with sheep kidney nuclease and acid RNAase from hog spleen revealed characteristically different fragmentation patterns for the native and denatured forms of the tRNAs. Apparently it is mainly the miniloop and dihydrouridine regions which are involved in the conformational changes responsible for denaturation.The rate constants, activation enthalpies, and activation entropies of the renaturation process were calculated from amino acid acceptance data.When the changes in hyperchromicity at 260 nm of the denatured tRNAs were followed as a function of time, a process was detected which in tRNAPheis 5–10 times and in tRNAser10–20 times faster than the renaturation as measured by acceptor activity. The whole net change in absorption, possibly representing the total net change in base stacking, occurs during the fast, process. The activation enthalpies and activation entropies are different for the fast and the over‐all processes.

Bibliography

Streeck, R. E., & Zachau, H. G. (1972). Characterization of the Native and Denatured Conformations of tRNASerand tRNAPhefrom Yeast. European Journal of Biochemistry, 30(2), 382â391. Portico.

Authors 2

Rolf E. Streeck (first)
Hans G. Zachau (additional)

References 30 Referenced 19

10.1073/pnas.55.4.941
10.1073/pnas.55.4.948
10.1073/pnas.57.6.1684
10.1016/0022-2836(69)90369-6
10.1016/0014-5793(71)80253-3
10.1515/bchm2.1966.347.1.212
10.1111/j.1432-1033.1968.tb00404.x
{'key': 'e_1_2_3_9_2', 'first-page': '37', 'volume-title': 'Procedures in Nucleic Acid Research', 'author': 'Bernardi A.', 'year': '1966'} / Procedures in Nucleic Acid Research by Bernardi A. (1966)
10.1111/j.1432-1033.1967.tb00057.x
Philippsen P.(1971)Thesis Universität München.
10.1515/9783112719336-065 / Methoden der enzymatischen Anulyse by Zachau H. G. (1970)
10.1111/j.1432-1033.1972.tb01749.x
10.1016/0014-5793(71)80081-9 / FEBS Lett. by Philimsen P. (1971)
10.1073/pnas.57.3.751
10.1016/S0021-9258(18)93646-X
Streeck R.‐E.(1971)Thesis Universität München.
10.1101/SQB.1966.031.01.054
Philippsen P.&Zachau H. G.(1972)Biochim. Biophys. Acta in press.
10.1042/bj0550170
10.1016/0014-5793(70)80558-0
10.1073/pnas.57.1.178 / Proc. Nat. Acad. Sci. U. S. A. by Lindahl A. (1966)
{'key': 'e_1_2_3_23_2', 'first-page': '657', 'volume': '30', 'author': 'Chirikjian J. G.', 'year': '1971', 'journal-title': 'Fed. Proc.'} / Fed. Proc. by Chirikjian J. G. (1971)
10.1063/1.1749604
10.1111/j.1432-1033.1971.tb01269.x
10.1016/0022-2836(71)90327-5
10.1073/pnas.62.4.1151
10.1016/0005-2787(70)90527-7
References deleted.
{'key': 'e_1_2_3_30_2', 'first-page': '1148', 'volume': '31', 'author': 'Uhlenbeck O.', 'year': '1972', 'journal-title': 'Fed. Proc.'} / Fed. Proc. by Uhlenbeck O. (1972)
Zachau H. G. Streeck R.‐E.&Hänggi U. J.(1971)XI Latin‐American Symposium International Symposium on Protein Synthesis and Nucleic Acids La Plata Argentina in press.

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 9:59 a.m.)
Deposited	8 months ago (Dec. 25, 2024, 10:03 a.m.)
Indexed	8 months ago (Dec. 25, 2024, 10:40 a.m.)
Issued	52 years, 10 months ago (Oct. 1, 1972)
Published	52 years, 10 months ago (Oct. 1, 1972)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	52 years, 10 months ago (Oct. 1, 1972)

Funders 0

None

BibTeX

@article{Streeck_1972, title={Characterization of the Native and Denatured Conformations of tRNASerand tRNAPhefrom Yeast}, volume={30}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1972.tb02109.x}, DOI={10.1111/j.1432-1033.1972.tb02109.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Streeck, Rolf E. and Zachau, Hans G.}, year={1972}, month=oct, pages={382–391} }

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