DOI: 10.1111/j.1432-1033.1972.tb02085.x. Study of the Position of NAD and Its Effect on the Conformation of <scp>d</scp>‐Glyceraldehyde‐3‐phosphate Dehydrogenase by Small‐Angle X‐Ray Scattering

Study of the Position of NAD and Its Effect on the Conformation of d‐Glyceraldehyde‐3‐phosphate Dehydrogenase by Small‐Angle X‐Ray Scattering

10.1111/j.1432-1033.1972.tb02085.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The structural changes of mammalian skeletal muscle d‐glyceraldehyde‐3‐phosphate dehydrogenase induced by the binding of the coenzyme, NAD, were studied by small‐angle X‐ray scattering with the double‐monitor technique. As shown by our difference method the radius of gyration of the apoenzyme decreases by 0.08 nm on the binding of four molecules of NAD. The decrease in the radius of gyration is not a monotonous function of saturation with NAD. From the analysis of our data it follows that, in the order of binding, the first and third NADs are positioned at a distance of about 2 nm from the centre of gravity of the molecule, whereas the second and fourth NADs are at a distance of about 4 nm. As indicated by the change in the radius of gyration the protomers of the tetrameric molecule are dislocated by about 0.1–0.2 nm (i.e. 1–4°) relative to each other.

Bibliography

Simon, I. (1972). Study of the Position of NAD and Its Effect on the Conformation of dâGlyceraldehydeâ3âphosphate Dehydrogenase by SmallâAngle XâRay Scattering. European Journal of Biochemistry, 30(1), 184â189. Portico.

Authors 1

István Simon (first)

References 22 Referenced 23

{'key': 'e_1_2_2_2_2', 'first-page': '199', 'volume': '13', 'author': 'Elődi P.', 'year': '1958', 'journal-title': 'Acta Physiol. Acad. Sci. Hung.'} / Acta Physiol. Acad. Sci. Hung. by Elődi P. (1958)
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10.1021/bi00830a020 / Biochemistry by Hoagland V.D. (1969)
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{'key': 'e_1_2_2_12_2', 'first-page': '215', 'volume': '5', 'author': 'Vas M.', 'year': '1970', 'journal-title': 'Acta Biochim. Biophys. Acad. Sci. Hung.'} / Acta Biochim. Biophys. Acad. Sci. Hung. by Vas M. (1970)
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{'key': 'e_1_2_2_14_2', 'first-page': '8779', 'volume': '10', 'author': 'Velick S. F.', 'year': '1971', 'journal-title': 'Biochemistry'} / Biochemistry by Velick S. F. (1971)
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{'key': 'e_1_2_2_21_2', 'first-page': '339', 'volume': '9', 'author': 'Elődi P.', 'year': '1956', 'journal-title': 'Acta Physiol. Acad. Sci. Hung.'} / Acta Physiol. Acad. Sci. Hung. by Elődi P. (1956)
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Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 9:57 a.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 4:47 p.m.)
Indexed	1 year, 6 months ago (Feb. 9, 2024, 3:48 p.m.)
Issued	52 years, 11 months ago (Oct. 1, 1972)
Published	52 years, 11 months ago (Oct. 1, 1972)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	52 years, 11 months ago (Oct. 1, 1972)

Funders 0

None

BibTeX

@article{Simon_1972, title={Study of the Position of NAD and Its Effect on the Conformation of <scp>d</scp>‐Glyceraldehyde‐3‐phosphate Dehydrogenase by Small‐Angle X‐Ray Scattering}, volume={30}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1972.tb02085.x}, DOI={10.1111/j.1432-1033.1972.tb02085.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Simon, István}, year={1972}, month=oct, pages={184–189} }

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