DOI: 10.1111/j.1432-1033.1972.tb02070.x. Synthesis of Lens Protein in <i>vitro</i> Role of Methionyl‐tRNAs in the Synthesis of Calf‐Lens α‐Crystallin

Synthesis of Lens Protein in vitro Role of Methionyl‐tRNAs in the Synthesis of Calf‐Lens α‐Crystallin

10.1111/j.1432-1033.1972.tb02070.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The main polypeptide chain of the lens protein α‐crystallin (αA2) is N‐terminally acetylated and contains two methionine residues. The chain is initiated by the incorporation of a methionine residue exclusively donated by methionyl‐tRNArMet.This N‐terminal methionine is not removed during polypeptide chain synthesis. The internal methionine residue is donated exclusively by one of the methionyl‐tRNAMet species. When lens messenger RNA coding for the αrA2 chain is translated in a reticulocyte cell‐free system, the newly synthesized polypeptide chain carries an acetylated methionine in the N‐terminal position.

Bibliography

Strous, G. J. A. M., Berns, T. J. M., van Westernen, H., & Bloemendal, H. (1972). Synthesis of Lens Protein in vitro Role of MethionylâtRNAs in the Synthesis of CalfâLens Î±âCrystallin. European Journal of Biochemistry, 30(1), 48â52. Portico.

Authors 4

Ger J. A. M. Strous (first)
Ton J. M. Berns (additional)
Hanske van Westernen (additional)
Hans Bloemendal (additional)

References 23 Referenced 18

10.1038/226607a0
10.1073/pnas.66.4.1282
10.1038/227676a0
10.1038/227672a0
10.1038/227913a0
10.1016/0006-291X(70)91023-5
10.1016/0014-5793(71)80599-9
10.1016/S0022-2836(64)80164-9
10.1111/j.1432-1033.1971.tb01321.x
10.1038/newbio236007a0
10.1038/2211118a0
10.1016/0006-291X(69)90738-4
10.1038/226414a0
10.1073/pnas.53.5.1147
10.1111/j.1399-3011.1969.tb01623.x
10.1016/S0021-9258(18)99854-6
10.1016/0005-2795(68)90106-2
10.1016/S0014-4835(68)80080-6
10.1016/0304-4165(65)90147-9
10.1042/bj1150789
10.1042/bj1240061 / Biochem. J. by Corran P. H. (1971)
Berns A. J. M. vanKraaikamp M. Bloemendal H.&Lane C.D.(1972)Proc. Nat. Acad. Sci. U.S.A. in the press.
10.1038/newbio235099a0

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 9:57 a.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 4:47 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 11:19 a.m.)
Issued	52 years, 10 months ago (Oct. 1, 1972)
Published	52 years, 10 months ago (Oct. 1, 1972)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	52 years, 10 months ago (Oct. 1, 1972)

Funders 0

None

BibTeX

@article{Strous_1972, title={Synthesis of Lens Protein in vitro Role of Methionyl‐tRNAs in the Synthesis of Calf‐Lens α‐Crystallin}, volume={30}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1972.tb02070.x}, DOI={10.1111/j.1432-1033.1972.tb02070.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Strous, Ger J. A. M. and Berns, Ton J. M. and van Westernen, Hanske and Bloemendal, Hans}, year={1972}, month=oct, pages={48–52} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T16:19:19Z",
    "timestamp": 1700756359187
  },
  "reference-count": 23,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            3,
            3
          ]
        ],
        "date-time": "2005-03-03T00:00:00Z",
        "timestamp": 1109808000000
      },
      "content-version": "vor",
      "delay-in-days": 11841,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1972,
        10
      ]
    ]
  },
  "abstract": "<jats:p>The main polypeptide chain of the lens protein \u03b1\u2010crystallin (\u03b1A<jats:sub>2</jats:sub>) is N\u2010terminally acetylated and contains two methionine residues. The chain is initiated by the incorporation of a methionine residue exclusively donated by methionyl\u2010tRNA<jats:sup>rMet</jats:sup>.</jats:p><jats:p>This N\u2010terminal methionine is not removed during polypeptide chain synthesis. The internal methionine residue is donated exclusively by one of the methionyl\u2010tRNA<jats:sup>Met</jats:sup> species. When lens messenger RNA coding for the \u03b1rA<jats:sub>2</jats:sub> chain is translated in a reticulocyte cell\u2010free system, the newly synthesized polypeptide chain carries an acetylated methionine in the N\u2010terminal position.</jats:p>",
  "DOI": "10.1111/j.1432-1033.1972.tb02070.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ],
    "date-time": "2005-03-03T14:57:52Z",
    "timestamp": 1109861872000
  },
  "page": "48-52",
  "source": "Crossref",
  "is-referenced-by-count": 18,
  "title": "Synthesis of Lens Protein in <i>vitro</i> Role of Methionyl\u2010tRNAs in the Synthesis of Calf\u2010Lens \u03b1\u2010Crystallin",
  "prefix": "10.1111",
  "volume": "30",
  "author": [
    {
      "given": "Ger J. A. M.",
      "family": "Strous",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Ton J. M.",
      "family": "Berns",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Hanske",
      "family": "van Westernen",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Hans",
      "family": "Bloemendal",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/226607a0"
    },
    {
      "key": "e_1_2_2_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.66.4.1282"
    },
    {
      "key": "e_1_2_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/227676a0"
    },
    {
      "key": "e_1_2_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/227672a0"
    },
    {
      "key": "e_1_2_2_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/227913a0"
    },
    {
      "key": "e_1_2_2_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(70)91023-5"
    },
    {
      "key": "e_1_2_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(71)80599-9"
    },
    {
      "key": "e_1_2_2_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-2836(64)80164-9"
    },
    {
      "key": "e_1_2_2_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1971.tb01321.x"
    },
    {
      "key": "e_1_2_2_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/newbio236007a0"
    },
    {
      "key": "e_1_2_2_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/2211118a0"
    },
    {
      "key": "e_1_2_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(69)90738-4"
    },
    {
      "key": "e_1_2_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/226414a0"
    },
    {
      "key": "e_1_2_2_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.53.5.1147"
    },
    {
      "key": "e_1_2_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-3011.1969.tb01623.x"
    },
    {
      "key": "e_1_2_2_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)99854-6"
    },
    {
      "key": "e_1_2_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2795(68)90106-2"
    },
    {
      "key": "e_1_2_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0014-4835(68)80080-6"
    },
    {
      "key": "e_1_2_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-4165(65)90147-9"
    },
    {
      "key": "e_1_2_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj1150789"
    },
    {
      "key": "e_1_2_2_22_2",
      "doi-asserted-by": "crossref",
      "first-page": "61",
      "DOI": "10.1042/bj1240061",
      "volume": "124",
      "author": "Corran P. H.",
      "year": "1971",
      "journal-title": "Biochem. J."
    },
    {
      "key": "e_1_2_2_23_2",
      "unstructured": "Berns A. J. M. vanKraaikamp M. Bloemendal H.&Lane C.D.(1972)Proc. Nat. Acad. Sci. U.S.A. in the press."
    },
    {
      "key": "e_1_2_2_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/newbio235099a0"
    }
  ],
  "container-title": "European Journal of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1972.tb02070.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1972.tb02070.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        22
      ]
    ],
    "date-time": "2023-11-22T21:47:13Z",
    "timestamp": 1700689633000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1972.tb02070.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1972,
        10
      ]
    ]
  },
  "references-count": 23,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1972,
          10
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1432-1033.1972.tb02070.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1972.tb02070.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1972,
        10
      ]
    ]
  }
}