DOI: 10.1111/j.1432-1033.1972.tb01789.x. Affinity Chromatography of DNA‐Binding Enzymes on Single‐Stranded DNA‐Agarose Columns

Affinity Chromatography of DNA‐Binding Enzymes on Single‐Stranded DNA‐Agarose Columns

10.1111/j.1432-1033.1972.tb01789.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Agarose gels containing immobilized single‐stranded circular DNA from phage fd or denatured calf thymus DNA were investigated for their use in the affinity chromatography of DNA‐binding enzymes. The DNA content of gel fragments is stable under the conventional conditions of enzyme purification. Single‐stranded DNA‐agarose columns have a high capacity to bind DNA‐specific proteins. They were used to differentiate between similar enzymatic activities in DNA‐free extracts from Escherichia coli. Preparative purification is described for the following enzymes: E. coli DNA polymerase I, DNA polymerase II, RNA polymerase, exonuclease III and T4 polynucleotide kinase. Enzyme purification was as high as 200‐fold, recovery of enzymatic activity was 75–100%.

Bibliography

Schaller, H., NÃ¼sslein, C., Bonhoeffer, F. J., Kurz, C., & Nietzschmann, I. (1972). Affinity Chromatography of DNAâBinding Enzymes on SingleâStranded DNAâAgarose Columns. European Journal of Biochemistry, 26(4), 474â481. Portico.

Authors 5

Heinz Schaller (first)
Christiane Nüsslein (additional)
Friedrich J. Bonhoeffer (additional)
Christina Kurz (additional)
Irmtraud Nietzschmann (additional)

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Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 9:42 a.m.)
Deposited	1 year, 10 months ago (Oct. 7, 2023, 3:56 a.m.)
Indexed	1 day, 5 hours ago (Sept. 4, 2025, 9:23 a.m.)
Issued	53 years, 5 months ago (April 1, 1972)
Published	53 years, 5 months ago (April 1, 1972)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	53 years, 5 months ago (April 1, 1972)

Funders 0

None

BibTeX

@article{Schaller_1972, title={Affinity Chromatography of DNA‐Binding Enzymes on Single‐Stranded DNA‐Agarose Columns}, volume={26}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1972.tb01789.x}, DOI={10.1111/j.1432-1033.1972.tb01789.x}, number={4}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Schaller, Heinz and Nüsslein, Christiane and Bonhoeffer, Friedrich J. and Kurz, Christina and Nietzschmann, Irmtraud}, year={1972}, month=apr, pages={474–481} }

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