Abstract
Phosphoglycerate kinase, isolated from rabbit muscle in highly purified crystalline form, was compared in terms of chemical, physical and kinetic properties to yeast phosphoglycerate kinase. A summary of the data is presented.Both proteins are composed of a single polypeptide chain, and in electrophoresis only one band was found. The N‐terminus is masked. The C‐terminal amino acid of the muscle enzyme is valine.Muscle and yeast enzyme have nearly identical molecular weights, Michaelis‐Menten constants, optimal pH values and Vmax. On the other hand, they differ significantly in their amino acid composition, especially in the content of sulfur and aromatic amino acids, in electrophoretic mobility and in heat stability. The muscle enzyme has an essential SH group. The single SH group of the yeast enzyme is apparently without importance for enzyme activity.Differences in the same direction as those observed with yeast and muscle phosphoglycerate kinase are discussed in connection with other glycolytic enzymes from the same organisms.
References
46
Referenced
193
{'key': 'e_1_2_3_2_2', 'first-page': '46', 'volume': '303', 'author': 'Warburg O.', 'year': '1939', 'journal-title': 'Biochem. Z.'}
/ Biochem. Z. by Warburg O. (1939)10.1007/BF01488658
10.1016/0006-3002(47)90143-1
10.1093/oxfordjournals.jbchem.a127990
/ J. Biochem. (Tokyo) by Hashimoto T. (1964)10.1016/S0021-9258(18)66097-1
/ J. Biol. Chem. by Axelrod B. (1953)10.1016/0006-3002(63)91802-X
10.1515/znb-1953-1005
10.1016/S0065-3233(08)60311-3
10.1042/bj0810405
{'key': 'e_1_2_3_11_2', 'first-page': '1187', 'volume': '30', 'author': 'Awramow I. A.', 'year': '1965', 'journal-title': 'Biokhimiya'}
/ Biokhimiya by Awramow I. A. (1965){'key': 'e_1_2_3_12_2', 'first-page': '1236', 'volume': '348', 'author': 'Krietsch W. K. G.', 'year': '1967', 'journal-title': "Hoppe-Seyler's Z. Physiol. Chem."}
/ Hoppe-Seyler's Z. Physiol. Chem. by Krietsch W. K. G. (1967)- Krietsch W. K. G. Dissertation Faculty of Science University of Munich1970.
10.1042/bj1130551
10.1021/bi00859a010
10.1111/j.1432-1033.1970.tb00289.x
{'key': 'e_1_2_3_17_2', 'volume-title': 'Disc Electrophoresis, Distillation Prod. Div.', 'author': 'Davis B. J.', 'year': '1962'}
/ Disc Electrophoresis, Distillation Prod. Div. by Davis B. J. (1962)10.1111/j.1749-6632.1964.tb14229.x
10.1007/BF00592760
{'key': 'e_1_2_3_20_2', 'first-page': '292', 'volume-title': 'Hoppe‐Seyler/Thierfelder', 'author': 'Bücher Th.', 'year': '1964'}
/ Hoppe‐Seyler/Thierfelder by Bücher Th. (1964)10.1016/S0021-9258(18)51706-3
10.1021/bi00899a030
/ Biochemistry by Eys J. (1964)10.1021/bi00886a032
10.1016/S0021-9258(19)63409-5
10.1021/bi00869a018
10.1021/bi00868a038
10.1042/bj0960595
10.1111/j.1749-6632.1960.tb20055.x
10.1021/ja01646a025
10.1016/0003-9861(59)90090-6
10.1016/0076-6879(63)06257-1
{'key': 'e_1_2_3_32_2', 'first-page': '65', 'volume-title': 'Methods in Enzymology', 'author': 'Schachmann H. K.', 'year': '1957'}
/ Methods in Enzymology by Schachmann H. K. (1957)10.1016/0006-291X(68)90622-0
{'key': 'e_1_2_3_34_2', 'first-page': '532', 'volume-title': 'Methods in Enzymology', 'author': 'Gurd F. R. N.', 'year': '1967'}
/ Methods in Enzymology by Gurd F. R. N. (1967)- Sebald W. andKrietsch W. K. G. unpublished results.
10.1016/0005-2795(67)90005-0
10.1016/0003-2697(65)90026-6
10.1042/bj0890379
10.1159/000457210
- Ling N. S. Ph. D. Thesis University of Wisconsin1957.
{'key': 'e_1_2_3_41_2', 'first-page': '60', 'volume': '85', 'author': 'Larsson‐Raznikiewicz M.', 'year': '1964', 'journal-title': 'Biochim. Biophys. Acta'}
/ Biochim. Biophys. Acta by Larsson‐Raznikiewicz M. (1964)10.1016/0005-2744(67)90189-1
10.1016/0003-9861(61)90223-5
10.1016/S0021-9258(18)93502-7
/ J. Biol. Chem. by Alpers J. B. (1968)10.1042/bj1070139
10.1007/BF00520353
10.1111/j.1432-1033.1970.tb01043.x
Dates
Type | When |
---|---|
Created | 20 years, 6 months ago (March 3, 2005, 9:01 a.m.) |
Deposited | 1 year, 11 months ago (Oct. 1, 2023, 11:20 a.m.) |
Indexed | 43 minutes ago (Sept. 4, 2025, 2:24 a.m.) |
Issued | 54 years, 9 months ago (Dec. 1, 1970) |
Published | 54 years, 9 months ago (Dec. 1, 1970) |
Published Online | 20 years, 6 months ago (March 3, 2005) |
Published Print | 54 years, 9 months ago (Dec. 1, 1970) |
@article{Krietsch_1970, title={3‐Phosphoglycerate Kinase from Rabbit Sceletal Muscle and Yeast}, volume={17}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1970.tb01202.x}, DOI={10.1111/j.1432-1033.1970.tb01202.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Krietsch, Wolfgang K. G. and Bücher, Theodor}, year={1970}, month=dec, pages={568–580} }