DOI: 10.1111/j.1432-1033.1970.tb01113.x. Cooperative Binding of Manganese (II) to Transfer RNA

Cooperative Binding of Manganese (II) to Transfer RNA

10.1111/j.1432-1033.1970.tb01113.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

We have measured the binding of manganse ions to tRNA in the concentration range of 1 to 28 bound Mn2+ per tRNA at 23°. The free Mn2+ concentration was monitored by electron spin resonance. The Scatchard plot shows that the first four Mn2+ bind cooperatively, at a free Mn2+ concentration of 3 μM. The cooperativity presumably reflects a conformational change of tRNA. A resulting structure with 6–10 strong binding sites (ks= 1.8 × 105M−1), and 21–25 weak binding sites (kw= 5 × 103 M−1) is suggested.From the bound Mn2+ electron spin resonance we estimate a minimal distance between the strong sites of about 1.5 nm; this shows that the binding sites are spread on the molecule and not clustered together.

Bibliography

Danchin, A., & GuÃ¨ron, M. (1970). Cooperative Binding of Manganese (II) to Transfer RNA. European Journal of Biochemistry, 16(3), 532â536. Portico.

Authors 2

Antoine Danchin (first)
Maurice Guèron (additional)

References 11 Referenced 76

10.1002/anie.196907111
{'key': 'e_1_2_1_3_2', 'first-page': '298', 'volume-title': 'Polynucleotides', 'author': 'Steiner R. F.', 'year': '1961'} / Polynucleotides by Steiner R. F. (1961)
10.1101/SQB.1966.031.01.068
10.1101/SQB.1966.031.01.074
10.1063/1.1695717
10.1016/0022-2836(69)90342-8
10.1038/1731090b0
Danchin A. andGuéron M. J.Chem. Phys.(in the press) (1970).
10.1021/j150466a007
Abragam A. The Principles of Nuclear Magnetism Oxford1961 p.112.
10.1073/pnas.65.3.638

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 7:49 a.m.)
Deposited	1 year, 10 months ago (Oct. 1, 2023, 3:45 p.m.)
Indexed	1 year, 7 months ago (Jan. 19, 2024, 10:40 a.m.)
Issued	54 years, 9 months ago (Nov. 1, 1970)
Published	54 years, 9 months ago (Nov. 1, 1970)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	54 years, 9 months ago (Nov. 1, 1970)

Funders 0

None

BibTeX

@article{Danchin_1970, title={Cooperative Binding of Manganese (II) to Transfer RNA}, volume={16}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1970.tb01113.x}, DOI={10.1111/j.1432-1033.1970.tb01113.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Danchin, Antoine and Guèron, Maurice}, year={1970}, month=nov, pages={532–536} }

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  "abstract": "<jats:p>We have measured the binding of manganse ions to tRNA in the concentration range of 1 to 28 bound Mn<jats:sup>2+</jats:sup> per tRNA at 23\u00b0. The free Mn<jats:sup>2+</jats:sup> concentration was monitored by electron spin resonance. The Scatchard plot shows that the first four Mn<jats:sup>2+</jats:sup> bind cooperatively, at a free Mn<jats:sup>2+</jats:sup> concentration of 3 \u03bcM. The cooperativity presumably reflects a conformational change of tRNA. A resulting structure with 6\u201310 strong binding sites (<jats:italic>k<jats:sub>s</jats:sub></jats:italic>= 1.8 \u00d7 10<jats:sup>5</jats:sup>M<jats:sup>\u22121</jats:sup>), and 21\u201325 weak binding sites (<jats:italic>k<jats:sub>w</jats:sub></jats:italic>= 5 \u00d7 10<jats:sup>3</jats:sup> M<jats:sup>\u22121</jats:sup>) is suggested.</jats:p><jats:p>From the bound Mn<jats:sup>2+</jats:sup> electron spin resonance we estimate a minimal distance between the strong sites of about 1.5 nm; this shows that the binding sites are spread on the molecule and not clustered together.</jats:p>",
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