DOI: 10.1111/j.1432-1033.1970.tb01091.x. Oxidation of 5β‐Cholestane‐3α, 7α, 12α‐triol by Rat Liver Microsomes

Oxidation of 5β‐Cholestane‐3α, 7α, 12α‐triol by Rat Liver Microsomes

10.1111/j.1432-1033.1970.tb01091.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The oxidation of 5β‐cholestane‐3α, 7α, 12α‐triol by different subcellular fractions of rat liver homogenate was studied. The most efficient oxidation was observed with the microsomal fraction fortified with the 100000 ×g supernatant fluid. Efficient oxidation occurred also with the microsomal fraction fortified with NADPH. The main products were identified as a number of cholestanetetrols. 5β‐Cholestane‐3α, 7α, 12α,25‐tetrol and 5β‐cholestane‐3α, 7α, 12α, 26‐tetrol accounted for about 60% of the cholestanetetrols. The oxidation of 5β‐cholestane‐3α, 7α, 12α‐triol by the microsomal fraction fortified with NADPH was stimulated about five‐fold by administration of phenobarbital. Phenobarbital treatment stimulated hydroxylations at C‐23, C‐24 and C‐25 to a much greater extent than that at C‐26. Carbon monoxide inhibited all the side‐chain hydroxylations and the most marked inhibition was observed for the 26‐hydroxylation.5β‐Cholestane‐3α, 7α, 12α, 26‐tetrol was found to be a much more efficient precursor of cholic acid than either 5β‐cholestane‐3α, 7α, 12α, 25‐tetrol or the two C‐24 epimers of 5β‐cholestane‐3α, 7α, 12α, 24‐tetrol.

Bibliography

Cronholm, T., & Johansson, G. (1970). Oxidation of 5Î²âCholestaneâ3Î±, 7Î±, 12Î±âtriol by Rat Liver Microsomes. European Journal of Biochemistry, 16(2), 373â381. Portico.

Authors 2

Tomas Cronholm (first)
Gunnar Johansson (additional)

References 31 Referenced 81

{'key': 'e_1_2_1_2_2', 'first-page': '279', 'volume-title': 'The Biological Basis of Medicine', 'author': 'Danielsson H.', 'year': '1969'} / The Biological Basis of Medicine by Danielsson H. (1969)
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{'key': 'e_1_2_1_26_2', 'first-page': '375', 'volume': '15', 'author': 'Yamada T.', 'year': '1966', 'journal-title': 'Hiroshima J. Med. Sci.'} / Hiroshima J. Med. Sci. by Yamada T. (1966)
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{'key': 'e_1_2_1_32_2', 'first-page': '97', 'volume-title': 'Symposium on the Interaction of Drugs and Subcellular Components in Animal Cells', 'author': 'Orrenius S.', 'year': '1968'} / Symposium on the Interaction of Drugs and Subcellular Components in Animal Cells by Orrenius S. (1968)

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 7:44 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 3:16 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 3:30 p.m.)
Issued	54 years, 10 months ago (Oct. 1, 1970)
Published	54 years, 10 months ago (Oct. 1, 1970)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	54 years, 10 months ago (Oct. 1, 1970)

Funders 0

None

BibTeX

@article{Cronholm_1970, title={Oxidation of 5β‐Cholestane‐3α, 7α, 12α‐triol by Rat Liver Microsomes}, volume={16}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1970.tb01091.x}, DOI={10.1111/j.1432-1033.1970.tb01091.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Cronholm, Tomas and Johansson, Gunnar}, year={1970}, month=oct, pages={373–381} }

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