DOI: 10.1111/j.1432-1033.1970.tb01008.x. Immunochemical Studies on Structural Proteins of the Red Cell Membrane

Immunochemical Studies on Structural Proteins of the Red Cell Membrane

10.1111/j.1432-1033.1970.tb01008.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

Up to one third of the membrane proteins from bovine, ovine and human red cells were solubilized similarily by water pH 9.5, aqueous pyridine, ATP‐mercaptoethanol solutions or urea pH 11. The proteins are heterogeneous but show some common features independent of the extraction method applied. The remaining insoluble proteins are rather different and contain most of the lipids and of the surface antigens. In the extracts mainly a common membrane‐specific antigen of high anodic mobility was demonstrated which contains neither sialic acids nor lipids. This antigen is species‐specific and is not located on the membrane outside. It is not exposed to the surface after proteolytic treatment of intact red cells. The extracts are contaminated by traces of plasma proteins and surface antigens. The membrane antigen can be correlated to a characteristic double band demonstrable in the stroma by disc electrophoresis in the presence of sodium dodecylsulfate. The molecular weights were estimated as 190 000 and 210 000, respectively. Evidence is provided that these components represent aggregates.

Bibliography

Furthmayr, H., & Timpl, R. (1970). Immunochemical Studies on Structural Proteins of the Red Cell Membrane. European Journal of Biochemistry, 15(2), 301â310. Portico.

Authors 2

Heinz Furthmayr (first)
Rupert Timpl (additional)

References 32 Referenced 32

10.1021/bi00856a020
10.1073/pnas.61.3.1005
10.1126/science.159.3811.203
10.1007/BF01900755
10.1016/S0021-9258(18)93538-6
10.1016/0304-4165(66)90166-8
10.1016/0006-291X(68)90471-3
10.4049/jimmunol.91.5.683 / J. Immunol. by Howe C. (1963)
10.1016/0014-4827(68)90412-6
10.4049/jimmunol.102.3.573 / J. Immunol. by Howe C. (1969)
{'key': 'e_1_2_1_12_2', 'first-page': '165', 'volume': '4', 'author': 'Poulik M. D.', 'year': '1969', 'journal-title': 'Clin. Exp. Immunol.'} / Clin. Exp. Immunol. by Poulik M. D. (1969)
10.1016/0005-2736(69)90130-8
{'key': 'e_1_2_1_14_2', 'first-page': '151', 'volume-title': 'Laboratoriumsdiagnostik', 'author': 'Leipert T.', 'year': '1953'} / Laboratoriumsdiagnostik by Leipert T. (1953)
10.1016/S0021-9258(18)69851-5
10.1007/BF02045455
10.1016/0006-291X(67)90391-9
10.4049/jimmunol.91.3.323 / J. Immunol. by Bauer D. C. (1963)
{'key': 'e_1_2_1_19_2', 'volume-title': 'Immunodiffusion', 'author': 'Crowle A. J.', 'year': '1961'} / Immunodiffusion by Crowle A. J. (1961)
10.1159/000228215
{'key': 'e_1_2_1_21_2', 'first-page': '75', 'volume-title': 'Immuno‐elektrophoretische Analyse', 'author': 'Uriel J.', 'year': '1964'} / Immuno‐elektrophoretische Analyse by Uriel J. (1964)
10.1016/0019-2791(69)90178-5
10.1002/path.1700590112
{'key': 'e_1_2_1_24_2', 'first-page': '79', 'volume': '136', 'author': 'Uhlenbruck G.', 'year': '1968', 'journal-title': 'Z. Immunforsch.'} / Z. Immunforsch. by Uhlenbruck G. (1968)
10.1042/bj0850282
10.4049/jimmunol.72.5.360 / J. Immunol. by Stavitsky A. B. (1954)
10.1016/S0021-9258(18)94333-4
10.1016/S0021-9258(18)63635-X
10.1016/S0021-9258(19)73972-6
10.4049/jimmunol.84.1.93
10.1085/jgp.54.1.65 / J. Gen. Physiol. by Steers E. (1969)
10.1016/0005-2795(70)90050-4
10.1016/0003-9861(68)90035-0

Dates

Type	When
Created	20 years, 5 months ago (March 3, 2005, 7:41 a.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 10:43 p.m.)
Indexed	1 year, 9 months ago (Nov. 22, 2023, 11:10 p.m.)
Issued	55 years ago (Aug. 1, 1970)
Published	55 years ago (Aug. 1, 1970)
Published Online	20 years, 5 months ago (March 3, 2005)
Published Print	55 years ago (Aug. 1, 1970)

Funders 0

None

BibTeX

@article{Furthmayr_1970, title={Immunochemical Studies on Structural Proteins of the Red Cell Membrane}, volume={15}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1970.tb01008.x}, DOI={10.1111/j.1432-1033.1970.tb01008.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Furthmayr, Heinz and Timpl, Rupert}, year={1970}, month=aug, pages={301–310} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T04:10:27Z",
    "timestamp": 1700712627206
  },
  "reference-count": 32,
  "publisher": "Wiley",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            3,
            3
          ]
        ],
        "date-time": "2005-03-03T00:00:00Z",
        "timestamp": 1109808000000
      },
      "content-version": "vor",
      "delay-in-days": 12633,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1970,
        8
      ]
    ]
  },
  "abstract": "<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>Up to one third of the membrane proteins from bovine, ovine and human red cells were solubilized similarily by water pH 9.5, aqueous pyridine, ATP\u2010mercaptoethanol solutions or urea pH 11. The proteins are heterogeneous but show some common features independent of the extraction method applied. The remaining insoluble proteins are rather different and contain most of the lipids and of the surface antigens.</jats:p></jats:list-item>\n<jats:list-item><jats:p>In the extracts mainly a common membrane\u2010specific antigen of high anodic mobility was demonstrated which contains neither sialic acids nor lipids. This antigen is species\u2010specific and is not located on the membrane outside. It is not exposed to the surface after proteolytic treatment of intact red cells. The extracts are contaminated by traces of plasma proteins and surface antigens.</jats:p></jats:list-item>\n<jats:list-item><jats:p>The membrane antigen can be correlated to a characteristic double band demonstrable in the stroma by disc electrophoresis in the presence of sodium dodecylsulfate. The molecular weights were estimated as 190 000 and 210 000, respectively. Evidence is provided that these components represent aggregates.</jats:p></jats:list-item>\n</jats:list>\n</jats:p>",
  "DOI": "10.1111/j.1432-1033.1970.tb01008.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ],
    "date-time": "2005-03-03T12:41:31Z",
    "timestamp": 1109853691000
  },
  "page": "301-310",
  "source": "Crossref",
  "is-referenced-by-count": 32,
  "title": "Immunochemical Studies on Structural Proteins of the Red Cell Membrane",
  "prefix": "10.1111",
  "volume": "15",
  "author": [
    {
      "given": "Heinz",
      "family": "Furthmayr",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Rupert",
      "family": "Timpl",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00856a020"
    },
    {
      "key": "e_1_2_1_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.61.3.1005"
    },
    {
      "key": "e_1_2_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.159.3811.203"
    },
    {
      "key": "e_1_2_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01900755"
    },
    {
      "key": "e_1_2_1_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)93538-6"
    },
    {
      "key": "e_1_2_1_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-4165(66)90166-8"
    },
    {
      "key": "e_1_2_1_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(68)90471-3"
    },
    {
      "key": "e_1_2_1_9_2",
      "doi-asserted-by": "crossref",
      "first-page": "683",
      "DOI": "10.4049/jimmunol.91.5.683",
      "volume": "91",
      "author": "Howe C.",
      "year": "1963",
      "journal-title": "J. Immunol."
    },
    {
      "key": "e_1_2_1_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-4827(68)90412-6"
    },
    {
      "key": "e_1_2_1_11_2",
      "doi-asserted-by": "crossref",
      "first-page": "573",
      "DOI": "10.4049/jimmunol.102.3.573",
      "volume": "102",
      "author": "Howe C.",
      "year": "1969",
      "journal-title": "J. Immunol."
    },
    {
      "key": "e_1_2_1_12_2",
      "first-page": "165",
      "volume": "4",
      "author": "Poulik M. D.",
      "year": "1969",
      "journal-title": "Clin. Exp. Immunol."
    },
    {
      "key": "e_1_2_1_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(69)90130-8"
    },
    {
      "key": "e_1_2_1_14_2",
      "first-page": "151",
      "volume-title": "Laboratoriumsdiagnostik",
      "author": "Leipert T.",
      "year": "1953"
    },
    {
      "key": "e_1_2_1_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)69851-5"
    },
    {
      "key": "e_1_2_1_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02045455"
    },
    {
      "key": "e_1_2_1_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(67)90391-9"
    },
    {
      "key": "e_1_2_1_18_2",
      "doi-asserted-by": "crossref",
      "first-page": "323",
      "DOI": "10.4049/jimmunol.91.3.323",
      "volume": "91",
      "author": "Bauer D. C.",
      "year": "1963",
      "journal-title": "J. Immunol."
    },
    {
      "key": "e_1_2_1_19_2",
      "volume-title": "Immunodiffusion",
      "author": "Crowle A. J.",
      "year": "1961"
    },
    {
      "key": "e_1_2_1_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1159/000228215"
    },
    {
      "key": "e_1_2_1_21_2",
      "first-page": "75",
      "volume-title": "Immuno\u2010elektrophoretische Analyse",
      "author": "Uriel J.",
      "year": "1964"
    },
    {
      "key": "e_1_2_1_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0019-2791(69)90178-5"
    },
    {
      "key": "e_1_2_1_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/path.1700590112"
    },
    {
      "key": "e_1_2_1_24_2",
      "first-page": "79",
      "volume": "136",
      "author": "Uhlenbruck G.",
      "year": "1968",
      "journal-title": "Z. Immunforsch."
    },
    {
      "key": "e_1_2_1_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj0850282"
    },
    {
      "key": "e_1_2_1_26_2",
      "doi-asserted-by": "crossref",
      "first-page": "360",
      "DOI": "10.4049/jimmunol.72.5.360",
      "volume": "72",
      "author": "Stavitsky A. B.",
      "year": "1954",
      "journal-title": "J. Immunol."
    },
    {
      "key": "e_1_2_1_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)94333-4"
    },
    {
      "key": "e_1_2_1_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)63635-X"
    },
    {
      "key": "e_1_2_1_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)73972-6"
    },
    {
      "key": "e_1_2_1_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.4049/jimmunol.84.1.93"
    },
    {
      "key": "e_1_2_1_31_2",
      "doi-asserted-by": "crossref",
      "first-page": "65",
      "DOI": "10.1085/jgp.54.1.65",
      "volume": "54",
      "author": "Steers E.",
      "year": "1969",
      "journal-title": "J. Gen. Physiol."
    },
    {
      "key": "e_1_2_1_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2795(70)90050-4"
    },
    {
      "key": "e_1_2_1_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-9861(68)90035-0"
    }
  ],
  "container-title": "European Journal of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1970.tb01008.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1970.tb01008.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T03:43:26Z",
    "timestamp": 1700711006000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1970.tb01008.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1970,
        8
      ]
    ]
  },
  "references-count": 32,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1970,
          8
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1432-1033.1970.tb01008.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1970.tb01008.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1970,
        8
      ]
    ]
  }
}