DOI: 10.1111/j.1432-1033.1970.tb00827.x. Separation of Large Quantities of Ribosomal Subunits by Zonal Ultracentrifugation

Separation of Large Quantities of Ribosomal Subunits by Zonal Ultracentrifugation

10.1111/j.1432-1033.1970.tb00827.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The maximum capacity of the B‐XV zonal rotor for the separation of Escherichia coli ribosomal subunits has been investigated. These experiments were performed using a class of sucrose density gradients (hyperbolic gradients) which present certain theoretical advantages. 2 g of subunits could be utilised in single centrifugations with a final yield of 0.5 g of 30 S and 1.0 g of 50 S subunits of greater than 99° purity.

Bibliography

Eikenberry, E. F., Bickle, T. A., Traut, R. R., & Price, C. A. (1970). Separation of Large Quantities of Ribosomal Subunits by Zonal Ultracentrifugation. European Journal of Biochemistry, 12(1), 113â116. Portico.

Authors 4

E. F. Eikenberry (first)
T. A. Bickle (additional)
R. R. Traut (additional)
C. A. Price (additional)

References 10 Referenced 144

10.1073/pnas.57.5.1294
10.1021/j100816a039
10.1126/science.152.3720.378
10.1016/S0022-2836(59)80029-2
10.1016/0304-4165(67)90152-3
10.1126/science.131.3392.32
{'key': 'e_1_2_1_8_2', 'first-page': '193', 'volume-title': 'A Laboratory Manual of Analytical Methods of Protein Chemistry', 'author': 'Svensson H.', 'year': '1960'} / A Laboratory Manual of Analytical Methods of Protein Chemistry by Svensson H. (1960)
{'key': 'e_1_2_1_9_2', 'first-page': '41', 'article-title': 'The Developement of Zonal Centrifuges', 'volume': '21', 'author': 'Berman A. S.', 'year': '1966', 'journal-title': 'Nat. Cancer Inst. Monograph'} / Nat. Cancer Inst. Monograph / The Developement of Zonal Centrifuges by Berman A. S. (1966)
{'key': 'e_1_2_1_10_2', 'first-page': '219', 'volume': '21', 'author': 'Barber E. J.', 'year': '1966', 'journal-title': 'Nat. Cancer Inst. Monograph'} / Nat. Cancer Inst. Monograph by Barber E. J. (1966)
10.1016/0304-4165(67)90255-3

Dates

Type	When
Created	20 years, 5 months ago (March 4, 2005, 5:21 a.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 11:45 p.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 10:58 a.m.)
Issued	55 years, 7 months ago (Jan. 1, 1970)
Published	55 years, 7 months ago (Jan. 1, 1970)
Published Online	17 years, 2 months ago (June 28, 2008)
Published Print	55 years, 7 months ago (Jan. 1, 1970)

Funders 0

None

BibTeX

@article{Eikenberry_1970, title={Separation of Large Quantities of Ribosomal Subunits by Zonal Ultracentrifugation}, volume={12}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1970.tb00827.x}, DOI={10.1111/j.1432-1033.1970.tb00827.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Eikenberry, E. F. and Bickle, T. A. and Traut, R. R. and Price, C. A.}, year={1970}, month=jan, pages={113–116} }

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