DOI: 10.1111/j.1432-1033.1969.tb19626.x. Ion Transport in Liver Mitochondria

10.1111/j.1432-1033.1969.tb19626.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The relationship between translocation of K+ and respiration has been studied under conditions where large variations of the transmembrane K+ concentration gradient were obtained. It is found that the energy expenditure is a function of the number of ions translocated and not of the transmembrane concentration gradient. The K+/∼ ratio is slightly below 3 when measured on the total oxygen uptake and between 3 and 4 when measured on the Δ oxygen uptake. The maximal [K+]i/[K+]o ratio maintained by respiring liver mitochondria is about 1,600 corresponding to an equilibrium potential of 192 mV. When the [K+]i/[K+]o ratio exceeds 2,000 times addition of valinomycin under aerobic conditions causes an efflux instead of an uptake of K+. Mitochondria behave as perfect osmometers in a wide range of osmolarities. It can be calculated that the concentration of osmotically active internal K+ corresponds to 45% of the total osmolarity of the medium.

Bibliography

Rossi, E., & Azzone, G. F. (1969). Ion Transport in Liver Mitochondria. European Journal of Biochemistry, 7(3), 418â426. Portico.

Authors 2

E. Rossi (first)
G. F. Azzone (additional)

References 21 Referenced 68

10.1021/bi00871a022
10.1016/S0021-9258(18)91230-5
10.1016/S0021-9258(18)97387-4
10.1016/S0021-9258(18)93573-8 / J. Biol. Chem. by Rossi E. (1968)
10.1016/S0021-9258(18)99724-3
{'key': 'e_1_2_2_7_2', 'first-page': '1370', 'volume': '26', 'author': 'Mitchell P.', 'year': '1967', 'journal-title': 'Federation Proc.'} / Federation Proc. by Mitchell P. (1967)
Azzone G. F. Massari S. Rossi E. andScarpa A. FEBS Meet. Abstr. 5th Prague1968 in press.
Piemonte G. andAzzone G. F. in press (Bari 1968).
10.1016/S0021-9258(19)57189-7
10.1016/0005-2728(67)90135-1
10.1042/bj0990200
10.1085/jgp.50.6.1547
10.1016/S0926-6593(66)80003-6
10.1083/jcb.6.2.241
Rossi E. andAzzone G. F. unpublished observations.
10.1113/jphysiol.1961.sp006626
10.1152/physrev.1968.48.1.1
10.1016/S0021-9258(19)73805-8
10.1073/pnas.53.5.1069
10.1113/jphysiol.1967.sp008297
10.1111/j.1469-185X.1966.tb01501.x

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 10:16 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:54 a.m.)
Indexed	4 hours, 43 minutes ago (Sept. 4, 2025, 9:52 a.m.)
Issued	56 years, 8 months ago (Jan. 1, 1969)
Published	56 years, 8 months ago (Jan. 1, 1969)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	56 years, 8 months ago (Jan. 1, 1969)

Funders 0

None

BibTeX

@article{Rossi_1969, title={Ion Transport in Liver Mitochondria: Energy Barrier and Stoicheometry of Aerobic K+ Translocation}, volume={7}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1969.tb19626.x}, DOI={10.1111/j.1432-1033.1969.tb19626.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Rossi, E. and Azzone, G. F.}, year={1969}, month=jan, pages={418–426} }

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