DOI: 10.1111/j.1432-1033.1969.tb00805.x. The Threonine‐Sensitive Homoserine Dehydrogenase and Aspartokinase Activities of <i>Escherichia coli</i> K 12

The Threonine‐Sensitive Homoserine Dehydrogenase and Aspartokinase Activities of Escherichia coli K 12

10.1111/j.1432-1033.1969.tb00805.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The stopped‐flow and temperature‐jump techniques are applied to the study of the relaxations of an allosteric protein, the aspartokinase I‐homoserine dehydrogenase I of E. coli K 12. A concerted conformation change is known to occur in this protein under the influence of aspartate, of the K+ ions and of threonine, all of which are effectors of the catalytic activities. The kinetics of the conformation change can be followed by making use of effects on the ultraviolet absorption and on the fluorescence of the protein itself. Stopped‐flow experiments demonstrate that the relaxation observed after addition of any of the three effectors described is an exponential process, which appears to correspond to the slower of two temperature relaxations. In the absence of added ligands, the rates of these relaxations are approximately 1000 sec–1 and 14 sec–1 at 28°. The effects of the ligands on the processes observed in the stopped‐flow and in the temperature‐jump experiments are interpreted and the mechanism of the conformation change is shown to include at least two isomerization steps.

Bibliography

Janin, J., & Iwatsubo, M. (1969). The ThreonineâSensitive Homoserine Dehydrogenase and Aspartokinase Activities of Escherichia coli K 12. European Journal of Biochemistry, 11(3), 530â540. Portico.

Authors 2

Joël Janin (first)
M. Iwatsubo (additional)

References 15 Referenced 28

{'key': 'e_1_2_2_2_2', 'first-page': '364', 'volume-title': 'Regulation of Nucleic Acid and Protein Biosynthesis', 'author': 'Cohen G. N.', 'year': '1957'} / Regulation of Nucleic Acid and Protein Biosynthesis by Cohen G. N. (1957)
10.1111/j.1432-1033.1969.tb00804.x
10.1016/S0022-2836(65)80285-6
{'key': 'e_1_2_2_5_2', 'first-page': '895', 'volume-title': 'Techniques of Organic Chemistry', 'author': 'Eigen M.', 'year': '1963'} / Techniques of Organic Chemistry by Eigen M. (1963)
10.1111/j.1432-1033.1969.tb00505.x
{'key': 'e_1_2_2_7_2', 'first-page': '333', 'volume-title': 'Nobel Symposium', 'author': 'Eigen M.', 'year': '1967'} / Nobel Symposium by Eigen M. (1967)
10.1016/S0065-3233(08)60399-X
10.1073/pnas.56.6.1661
10.1101/SQB.1963.028.01.068
10.1073/pnas.60.4.1363
Janin J. unpublished observations.
10.1016/S0065-3233(08)60401-5
{'key': 'e_1_2_2_14_2', 'first-page': '39', 'volume-title': 'Regulation of Enzyme Activity and Allosteric Interactions', 'author': 'Kirschner K.', 'year': '1968'} / Regulation of Enzyme Activity and Allosteric Interactions by Kirschner K. (1968)
10.1016/0022-2836(68)90294-5
10.1021/bi00865a047

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 11:47 a.m.)
Deposited	1 year, 11 months ago (Oct. 1, 2023, 2 p.m.)
Indexed	1 year, 7 months ago (Jan. 26, 2024, 6:01 p.m.)
Issued	55 years, 9 months ago (Dec. 1, 1969)
Published	55 years, 9 months ago (Dec. 1, 1969)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	55 years, 9 months ago (Dec. 1, 1969)

Funders 0

None

BibTeX

@article{Janin_1969, title={The Threonine‐Sensitive Homoserine Dehydrogenase and Aspartokinase Activities of Escherichia coli K 12: Relaxations of the Allosteric Equilibrium}, volume={11}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1969.tb00805.x}, DOI={10.1111/j.1432-1033.1969.tb00805.x}, number={3}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Janin, Joël and Iwatsubo, M.}, year={1969}, month=dec, pages={530–540} }

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