DOI: 10.1111/j.1432-1033.1969.tb00771.x. Effect of ATP on the Oxidation of Succinate in Rat Brain Mitochondria

Effect of ATP on the Oxidation of Succinate in Rat Brain Mitochondria

10.1111/j.1432-1033.1969.tb00771.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The effect of ATP on the aerobic oxidation of succinate and on the succinic dehydrogenase activity of isolated rat brain mitochondria has been studied. The addition of dinitrophenol to brain mitochondria with succinate as substrate produced a small activating effect of oxygen uptake which was followed by inhibition of respiration; the further addition of ATP produced a high rate of respiration. This effect of ATP was enhanced by added Mg++ and oligomycin and was diminished, but not abolished, by inorganic phosphate. Experiments were conducted which indirectly indicated that oxaloacetate may be involved in the inhibition of the aerobic oxidation of succinate in fresh brain mitochondria incubated with dinitrophenol; however, attempts to find out if ATP removed oxaloacetate through a particular ATP requiring metabolic pathway were unsuccessful. In aged, sonicated, and frozen and thawed brain mitochondria incubated with dinitrophenol, ATP still activated the aerobic oxidation of succinate. The assay of succinic dehydrogenase (EC 1.3.99.1) in aged brain mitochondria revealed that ATP diminished the Km for succinate and increased the Vmax of succinic dehydrogenase. ATP also produced a shift in the pH optimum of succinic dehydrogenase and a partial protection of the enzyme against heat denaturation.

Bibliography

Tuena, M., GÃ³mezâPuyou, A., PeÃ±a, A., ChÃ¡vez, E., & Sandoval, F. (1969). Effect of ATP on the Oxidation of Succinate in Rat Brain Mitochondria. European Journal of Biochemistry, 11(2), 283â290. Portico.

Authors 5

M. Tuena (first)
A. Gómez‐Puyou (additional)
A. Peña (additional)
E. Chávez (additional)
F. Sandoval (additional)

References 27 Referenced 9

10.1016/S0021-9258(18)57122-2
10.1016/S0021-9258(19)52316-X / J. Biol. Chem. by Tyler D. B. (1955)
10.1038/187065b0
10.1016/S0021-9258(18)64207-3
10.1016/0006-291X(61)90252-2 / Biochem. Biophys. Res. Commun. by Schollmeyer P. (1961)
{'key': 'e_1_2_2_7_2', 'first-page': '202', 'volume-title': 'Regulation of Metabolic Processes in Mitochondria', 'author': 'Tager J. M.', 'year': '1966'} / Regulation of Metabolic Processes in Mitochondria by Tager J. M. (1966)
10.1016/0006-3002(63)90497-9
{'key': 'e_1_2_2_9_2', 'first-page': '71', 'volume-title': 'Biological Structure and Function', 'author': 'Chappell J. B.', 'year': '1961'} / Biological Structure and Function by Chappell J. B. (1961)
{'key': 'e_1_2_2_10_2', 'first-page': '1', 'volume': '58', 'author': 'Slater E. C.', 'year': '1952', 'journal-title': 'Chem. Weekblad'} / Chem. Weekblad by Slater E. C. (1952)
{'key': 'e_1_2_2_11_2', 'first-page': '535', 'volume-title': 'Regulation of Metabolic Processes in Mitochondria', 'author': 'Klingenberg M.', 'year': '1966'} / Regulation of Metabolic Processes in Mitochondria by Klingenberg M. (1966)
Åkerblom A. Ernster L. Lindberg O. andWeinbach E. C. Intern. Congr. Biochem. Abstr. VI New York1964 p.773.
10.1016/0005-2728(69)90091-7
10.1016/S0021-9258(18)56449-8
{'key': 'e_1_2_2_15_2', 'first-page': '229', 'volume-title': 'Methods in Enzymatic Analysis', 'author': 'Bergmeyer H. U.', 'year': '1965'} / Methods in Enzymatic Analysis by Bergmeyer H. U. (1965)
10.1042/bj0580699
10.1016/S0021-9258(18)65755-2 / J. Biol. Chem. by Kearney E. B. (1956)
10.1016/0006-291X(61)90251-0
10.1042/bj0750523
10.1016/0006-3002(60)90391-7
10.1016/0014-4827(61)90033-7
10.1016/S0021-9258(18)81269-8
10.1016/S0021-9258(18)65700-X
10.1016/S0021-9258(18)65817-X
10.1002/9780470719145.ch6 / Ciba Found. Symp. Regulation Cell Metab. by Lehninger A. L. (1959)
10.1016/S0021-9258(18)70623-6
10.1016/S0021-9258(18)65059-8
{'key': 'e_1_2_2_28_2', 'first-page': '65P', 'volume': '84', 'author': 'Slater E. C.', 'year': '1962', 'journal-title': 'Biochem. J.'} / Biochem. J. by Slater E. C. (1962)

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 7:31 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 2:31 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 3:10 a.m.)
Issued	55 years, 9 months ago (Dec. 1, 1969)
Published	55 years, 9 months ago (Dec. 1, 1969)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	55 years, 9 months ago (Dec. 1, 1969)

Funders 0

None

BibTeX

@article{Tuena_1969, title={Effect of ATP on the Oxidation of Succinate in Rat Brain Mitochondria}, volume={11}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1969.tb00771.x}, DOI={10.1111/j.1432-1033.1969.tb00771.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Tuena, M. and Gómez‐Puyou, A. and Peña, A. and Chávez, E. and Sandoval, F.}, year={1969}, month=dec, pages={283–290} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T08:10:13Z",
    "timestamp": 1700727013293
  },
  "reference-count": 27,
  "publisher": "Wiley",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            3,
            3
          ]
        ],
        "date-time": "2005-03-03T00:00:00Z",
        "timestamp": 1109808000000
      },
      "content-version": "vor",
      "delay-in-days": 12876,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1969,
        12
      ]
    ]
  },
  "abstract": "<jats:p>The effect of ATP on the aerobic oxidation of succinate and on the succinic dehydrogenase activity of isolated rat brain mitochondria has been studied.</jats:p><jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>The addition of dinitrophenol to brain mitochondria with succinate as substrate produced a small activating effect of oxygen uptake which was followed by inhibition of respiration; the further addition of ATP produced a high rate of respiration.</jats:p></jats:list-item>\n<jats:list-item><jats:p>This effect of ATP was enhanced by added Mg<jats:sup>++</jats:sup> and oligomycin and was diminished, but not abolished, by inorganic phosphate.</jats:p></jats:list-item>\n<jats:list-item><jats:p>Experiments were conducted which indirectly indicated that oxaloacetate may be involved in the inhibition of the aerobic oxidation of succinate in fresh brain mitochondria incubated with dinitrophenol; however, attempts to find out if ATP removed oxaloacetate through a particular ATP requiring metabolic pathway were unsuccessful.</jats:p></jats:list-item>\n<jats:list-item><jats:p>In aged, sonicated, and frozen and thawed brain mitochondria incubated with dinitrophenol, ATP still activated the aerobic oxidation of succinate.</jats:p></jats:list-item>\n<jats:list-item><jats:p>The assay of succinic dehydrogenase (EC 1.3.99.1) in aged brain mitochondria revealed that ATP diminished the <jats:italic>K</jats:italic><jats:sub><jats:italic>m</jats:italic></jats:sub> for succinate and increased the <jats:italic>V</jats:italic><jats:sub>max</jats:sub> of succinic dehydrogenase. ATP also produced a shift in the pH optimum of succinic dehydrogenase and a partial protection of the enzyme against heat denaturation.</jats:p></jats:list-item>\n</jats:list>\n</jats:p>",
  "DOI": "10.1111/j.1432-1033.1969.tb00771.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ],
    "date-time": "2005-03-03T12:31:56Z",
    "timestamp": 1109853116000
  },
  "page": "283-290",
  "source": "Crossref",
  "is-referenced-by-count": 9,
  "title": "Effect of ATP on the Oxidation of Succinate in Rat Brain Mitochondria",
  "prefix": "10.1111",
  "volume": "11",
  "author": [
    {
      "given": "M.",
      "family": "Tuena",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "A.",
      "family": "G\u00f3mez\u2010Puyou",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "A.",
      "family": "Pe\u00f1a",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "E.",
      "family": "Ch\u00e1vez",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "F.",
      "family": "Sandoval",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_2_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)57122-2"
    },
    {
      "key": "e_1_2_2_3_2",
      "doi-asserted-by": "crossref",
      "first-page": "395",
      "DOI": "10.1016/S0021-9258(19)52316-X",
      "volume": "216",
      "author": "Tyler D. B.",
      "year": "1955",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_2_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/187065b0"
    },
    {
      "key": "e_1_2_2_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)64207-3"
    },
    {
      "key": "e_1_2_2_6_2",
      "doi-asserted-by": "crossref",
      "first-page": "43",
      "DOI": "10.1016/0006-291X(61)90252-2",
      "volume": "4",
      "author": "Schollmeyer P.",
      "year": "1961",
      "journal-title": "Biochem. Biophys. Res. Commun."
    },
    {
      "key": "e_1_2_2_7_2",
      "first-page": "202",
      "volume-title": "Regulation of Metabolic Processes in Mitochondria",
      "author": "Tager J. M.",
      "year": "1966"
    },
    {
      "key": "e_1_2_2_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-3002(63)90497-9"
    },
    {
      "key": "e_1_2_2_9_2",
      "first-page": "71",
      "volume-title": "Biological Structure and Function",
      "author": "Chappell J. B.",
      "year": "1961"
    },
    {
      "key": "e_1_2_2_10_2",
      "first-page": "1",
      "volume": "58",
      "author": "Slater E. C.",
      "year": "1952",
      "journal-title": "Chem. Weekblad"
    },
    {
      "key": "e_1_2_2_11_2",
      "first-page": "535",
      "volume-title": "Regulation of Metabolic Processes in Mitochondria",
      "author": "Klingenberg M.",
      "year": "1966"
    },
    {
      "key": "e_1_2_2_12_2",
      "unstructured": "\u00c5kerblom A. Ernster L. Lindberg O. andWeinbach E. C. Intern. Congr. Biochem. Abstr. VI New York1964 p.773."
    },
    {
      "key": "e_1_2_2_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2728(69)90091-7"
    },
    {
      "key": "e_1_2_2_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)56449-8"
    },
    {
      "key": "e_1_2_2_15_2",
      "first-page": "229",
      "volume-title": "Methods in Enzymatic Analysis",
      "author": "Bergmeyer H. U.",
      "year": "1965"
    },
    {
      "key": "e_1_2_2_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj0580699"
    },
    {
      "key": "e_1_2_2_17_2",
      "doi-asserted-by": "crossref",
      "first-page": "963",
      "DOI": "10.1016/S0021-9258(18)65755-2",
      "volume": "219",
      "author": "Kearney E. B.",
      "year": "1956",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_2_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(61)90251-0"
    },
    {
      "key": "e_1_2_2_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj0750523"
    },
    {
      "key": "e_1_2_2_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-3002(60)90391-7"
    },
    {
      "key": "e_1_2_2_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-4827(61)90033-7"
    },
    {
      "key": "e_1_2_2_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)81269-8"
    },
    {
      "key": "e_1_2_2_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)65700-X"
    },
    {
      "key": "e_1_2_2_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)65817-X"
    },
    {
      "key": "e_1_2_2_25_2",
      "doi-asserted-by": "crossref",
      "first-page": "130",
      "DOI": "10.1002/9780470719145.ch6",
      "volume-title": "Ciba Found. Symp. Regulation Cell Metab.",
      "author": "Lehninger A. L.",
      "year": "1959"
    },
    {
      "key": "e_1_2_2_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)70623-6"
    },
    {
      "key": "e_1_2_2_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)65059-8"
    },
    {
      "key": "e_1_2_2_28_2",
      "first-page": "65P",
      "volume": "84",
      "author": "Slater E. C.",
      "year": "1962",
      "journal-title": "Biochem. J."
    }
  ],
  "container-title": "European Journal of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1969.tb00771.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1969.tb00771.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T07:31:54Z",
    "timestamp": 1700724714000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1969.tb00771.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1969,
        12
      ]
    ]
  },
  "references-count": 27,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1969,
          12
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1432-1033.1969.tb00771.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1969.tb00771.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1969,
        12
      ]
    ]
  }
}