DOI: 10.1111/j.1432-1033.1967.tb19515.x. Initiation of DNA‐Dependent RNA Synthesis and the Effect of Heparin on RNA Polymerase

Initiation of DNA‐Dependent RNA Synthesis and the Effect of Heparin on RNA Polymerase

10.1111/j.1432-1033.1967.tb19515.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

The kinetics of RNA synthesis, catalyzed by DNA‐dependent RNA polymerase from Escherichia coli, show a lag phase at high ionic strength or at low temperature when using a double‐stranded DNA as template. This lag phase increases with decreasing temperature at constant ionic strength or with increasing ionic strength at constant temperature. With single‐stranded DNA as template, no lag phase is observed. The length of the lag phase is greatly shortened by pre‐incubation of the enzyme with DNA at low Mg++ concentration, independent of the presence of substrate.Heparin is a strong inhibitor of free and DNA‐bound polymerase. Two heparin molecules are required for the inactivation of one enzyme molecule. Enzyme engaged in RNA synthesis is instantly inhibited on single‐stranded DNA but not on double‐stranded DNA. With the aid of this inhibitor it is shown that 10 minutes after the start of incubation, at 16°, all enzyme molecules have initiated RNA chains on double‐stranded DNA.

Bibliography

Walter, G., Zillig, W., Palm, P., & Fuchs, E. (1967). Initiation of DNAâDependent RNA Synthesis and the Effect of Heparin on RNA Polymerase. European Journal of Biochemistry, 3(2), 194â201. Portico.

Authors 4

G. Walter (first)
W. Zillig (additional)
P. Palm (additional)
E. Fuchs (additional)

References 18 Referenced 182

10.1016/0022-2836(66)90081-7
10.1016/0022-2836(66)90126-4
{'key': 'e_1_2_2_4_2', 'first-page': '36', 'volume': '91', 'author': 'Kadoya M.', 'year': '1964', 'journal-title': 'Biochim. Biophys. Acta.'} / Biochim. Biophys. Acta. by Kadoya M. (1964)
10.1016/S0022-2836(64)80219-9
10.1016/S0021-9258(18)97432-6
10.1073/pnas.54.6.1736
Palm P. Doctoral Dissertation Universität München1966.
{'key': 'e_1_2_2_9_2', 'first-page': '323', 'volume-title': 'Procedures in Nucleic Acid Research', 'author': 'Zillig W.', 'year': '1966'} / Procedures in Nucleic Acid Research by Zillig W. (1966)
10.1111/j.1432-1033.1967.tb19514.x
Fuchs E. Doctoral Dissertation Universität München1965.
10.1515/bchm2.1962.330.1.96
10.1016/S0022-2836(63)80024-8
10.1016/S0021-9258(18)51766-X
10.1007/BF00599644
10.1016/0005-2795(67)90531-4
10.1016/S0022-2836(64)80076-0
10.1073/pnas.56.3.1026
Millette R. L. unpublished observations 1967.

Dates

Type	When
Created	20 years, 6 months ago (March 4, 2005, 3:44 a.m.)
Deposited	1 year, 9 months ago (Nov. 22, 2023, 10:59 p.m.)
Indexed	9 hours, 10 minutes ago (Sept. 4, 2025, 9:57 a.m.)
Issued	57 years, 9 months ago (Dec. 1, 1967)
Published	57 years, 9 months ago (Dec. 1, 1967)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	57 years, 9 months ago (Dec. 1, 1967)

Funders 0

None

BibTeX

@article{Walter_1967, title={Initiation of DNA‐Dependent RNA Synthesis and the Effect of Heparin on RNA Polymerase}, volume={3}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1967.tb19515.x}, DOI={10.1111/j.1432-1033.1967.tb19515.x}, number={2}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Walter, G. and Zillig, W. and Palm, P. and Fuchs, E.}, year={1967}, month=dec, pages={194–201} }

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