DOI: 10.1111/j.1432-1033.1967.tb00110.x. The Determination of the Order of Lysine‐containing

The Determination of the Order of Lysine‐containing

10.1111/j.1432-1033.1967.tb00110.x

Crossref journal-article

Wiley

European Journal of Biochemistry (311)

Abstract

A new diagonal electrophoretic technique for determining the order of the lysine‐containing tryptic peptides of a protein is described. The protein is converted into its trifluoracetyl derivative, digested enzymatically (or chemically), and the resulting peptides separated by paper electrophoresis. The paper is then treated with ammonia vapour, which re‐exposes the ɛ‐amino groups of the lysine residues, and submitted to a second electrophoresis at right angles to the first direction. Peptides containing lysine residues, together with the N‐terminal peptide of the protein, are found to lie off a diagonal formed by all other peptides, whence they may be readily purified. A study of these peptides enables the order of the lysine‐containing tryptic peptides in the protein to be deduced. The technique has been successfully tested with insulin. When the method was applied to porcine pepsin, the four tryptic peptides isolated were easily ordered and the carboxyl‐terminal sequence of the protein shown to be Arg‐Gln‐Tyr‐Tyr‐Thr‐Val‐Phe‐Asp‐Arg‐Ala‐Asn‐Asn‐Lys‐Val‐Gly‐Leu‐Ala‐Pro‐Val‐Ala. The three basic amino acid residues in the molecule are thus found clustering towards the C‐terminus of the polypeptide chain. A common ancestral gene for porcine pepsin and bovine (calf) rennin is suggested by the close homology between the C‐terminal sequences of the two proteins.

Bibliography

Perham, R. N., & Jones, G. M. T. (1967). The Determination of the Order of Lysineâcontaining. European Journal of Biochemistry, 2(1), 84â89. Portico.

Authors 2

R. N. Perham (first)
G. M. T. Jones (additional)

References 23 Referenced 39

10.1021/bi00909a005
10.1042/bj1010214
10.1021/ja01136a010
10.1042/bj0600541
10.1007/BF00900846
10.1042/bj0550328
{'key': 'e_1_2_3_8_2', 'first-page': '244', 'volume': '22', 'author': 'Spackman D. H.', 'year': '1963', 'journal-title': 'Federation Proc.'} / Federation Proc. by Spackman D. H. (1963)
10.1042/bj0890379
10.1042/bj0890379
10.1042/bj0890349
10.1016/S0021-9258(18)65721-7
10.1515/bchm2.1958.309.1.219
10.1042/bj0390507
10.1126/science.129.3359.1340
10.1002/cber.19540870807
10.1085/jgp.42.3.553
10.1016/S0021-9258(18)99655-9
10.1016/0006-3002(57)90178-6
10.1016/0006-3002(57)90382-7
10.1016/S0926-6593(66)80165-0 / Biochim. Biophys. Acta by Kuznetzov Y. S. (1966)
{'key': 'e_1_2_3_22_2', 'first-page': '1070', 'volume': '29', 'author': 'Vaganova T. I.', 'year': '1964', 'journal-title': 'Biokhimiya'} / Biokhimiya by Vaganova T. I. (1964)
Dopheide T. A. A. Moore S. andStein W. H. J. Biol. Chem.(1967) (in press).
{'key': 'e_1_2_3_24_2', 'first-page': '143', 'volume': '35', 'author': 'Foltmann B.', 'year': '1966', 'journal-title': 'Compt. Rend. Trav. Lab. Carlsberg'} / Compt. Rend. Trav. Lab. Carlsberg by Foltmann B. (1966)

Dates

Type	When
Created	20 years, 6 months ago (March 3, 2005, 6:16 a.m.)
Deposited	1 year, 9 months ago (Nov. 23, 2023, 7:41 a.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 6:28 p.m.)
Issued	58 years, 2 months ago (July 1, 1967)
Published	58 years, 2 months ago (July 1, 1967)
Published Online	20 years, 6 months ago (March 3, 2005)
Published Print	58 years, 2 months ago (July 1, 1967)

Funders 0

None

BibTeX

@article{Perham_1967, title={The Determination of the Order of Lysine‐containing: Tryptic Peptides of Proteins by Diagonal Paper Electrophoresis A Carboxyl‐terminal Sequence for Pepsin}, volume={2}, ISSN={1432-1033}, url={http://dx.doi.org/10.1111/j.1432-1033.1967.tb00110.x}, DOI={10.1111/j.1432-1033.1967.tb00110.x}, number={1}, journal={European Journal of Biochemistry}, publisher={Wiley}, author={Perham, R. N. and Jones, G. M. T.}, year={1967}, month=jul, pages={84–89} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T23:28:37Z",
    "timestamp": 1700782117197
  },
  "reference-count": 23,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            3,
            3
          ]
        ],
        "date-time": "2005-03-03T00:00:00Z",
        "timestamp": 1109808000000
      },
      "content-version": "vor",
      "delay-in-days": 13760,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1967,
        7
      ]
    ]
  },
  "abstract": "<jats:p>\n<jats:list list-type=\"explicit-label\">\n<jats:list-item><jats:p>A new diagonal electrophoretic technique for determining the order of the lysine\u2010containing tryptic peptides of a protein is described. The protein is converted into its trifluoracetyl derivative, digested enzymatically (or chemically), and the resulting peptides separated by paper electrophoresis. The paper is then treated with ammonia vapour, which re\u2010exposes the \u025b\u2010amino groups of the lysine residues, and submitted to a second electrophoresis at right angles to the first direction. Peptides containing lysine residues, together with the N\u2010terminal peptide of the protein, are found to lie off a diagonal formed by all other peptides, whence they may be readily purified. A study of these peptides enables the order of the lysine\u2010containing tryptic peptides in the protein to be deduced.</jats:p></jats:list-item>\n<jats:list-item><jats:p>The technique has been successfully tested with insulin.</jats:p></jats:list-item>\n<jats:list-item><jats:p>When the method was applied to porcine pepsin, the four tryptic peptides isolated were easily ordered and the carboxyl\u2010terminal sequence of the protein shown to be Arg\u2010Gln\u2010Tyr\u2010Tyr\u2010Thr\u2010Val\u2010Phe\u2010Asp\u2010Arg\u2010Ala\u2010Asn\u2010Asn\u2010Lys\u2010Val\u2010Gly\u2010Leu\u2010Ala\u2010Pro\u2010Val\u2010Ala. The three basic amino acid residues in the molecule are thus found clustering towards the C\u2010terminus of the polypeptide chain.</jats:p></jats:list-item>\n<jats:list-item><jats:p>A common ancestral gene for porcine pepsin and bovine (calf) rennin is suggested by the close homology between the C\u2010terminal sequences of the two proteins.</jats:p></jats:list-item>\n</jats:list>\n</jats:p>",
  "DOI": "10.1111/j.1432-1033.1967.tb00110.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ],
    "date-time": "2005-03-03T11:16:39Z",
    "timestamp": 1109848599000
  },
  "page": "84-89",
  "source": "Crossref",
  "is-referenced-by-count": 39,
  "title": "The Determination of the Order of Lysine\u2010containing",
  "prefix": "10.1111",
  "volume": "2",
  "author": [
    {
      "given": "R. N.",
      "family": "Perham",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "G. M. T.",
      "family": "Jones",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        3,
        3
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_3_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00909a005"
    },
    {
      "key": "e_1_2_3_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj1010214"
    },
    {
      "key": "e_1_2_3_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/ja01136a010"
    },
    {
      "key": "e_1_2_3_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj0600541"
    },
    {
      "key": "e_1_2_3_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00900846"
    },
    {
      "key": "e_1_2_3_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj0550328"
    },
    {
      "key": "e_1_2_3_8_2",
      "first-page": "244",
      "volume": "22",
      "author": "Spackman D. H.",
      "year": "1963",
      "journal-title": "Federation Proc."
    },
    {
      "key": "e_1_2_3_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj0890379"
    },
    {
      "key": "e_1_2_3_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj0890379"
    },
    {
      "key": "e_1_2_3_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj0890349"
    },
    {
      "key": "e_1_2_3_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)65721-7"
    },
    {
      "key": "e_1_2_3_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1515/bchm2.1958.309.1.219"
    },
    {
      "key": "e_1_2_3_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj0390507"
    },
    {
      "key": "e_1_2_3_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.129.3359.1340"
    },
    {
      "key": "e_1_2_3_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/cber.19540870807"
    },
    {
      "key": "e_1_2_3_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1085/jgp.42.3.553"
    },
    {
      "key": "e_1_2_3_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)99655-9"
    },
    {
      "key": "e_1_2_3_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-3002(57)90178-6"
    },
    {
      "key": "e_1_2_3_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-3002(57)90382-7"
    },
    {
      "key": "e_1_2_3_21_2",
      "doi-asserted-by": "crossref",
      "first-page": "219",
      "DOI": "10.1016/S0926-6593(66)80165-0",
      "volume": "118",
      "author": "Kuznetzov Y. S.",
      "year": "1966",
      "journal-title": "Biochim. Biophys. Acta"
    },
    {
      "key": "e_1_2_3_22_2",
      "first-page": "1070",
      "volume": "29",
      "author": "Vaganova T. I.",
      "year": "1964",
      "journal-title": "Biokhimiya"
    },
    {
      "key": "e_1_2_3_23_2",
      "unstructured": "Dopheide T. A. A. Moore S. andStein W. H. J. Biol. Chem.(1967) (in press)."
    },
    {
      "key": "e_1_2_3_24_2",
      "first-page": "143",
      "volume": "35",
      "author": "Foltmann B.",
      "year": "1966",
      "journal-title": "Compt. Rend. Trav. Lab. Carlsberg"
    }
  ],
  "container-title": "European Journal of Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1432-1033.1967.tb00110.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/pdf/10.1111/j.1432-1033.1967.tb00110.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        23
      ]
    ],
    "date-time": "2023-11-23T12:41:29Z",
    "timestamp": 1700743289000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://febs.onlinelibrary.wiley.com/doi/10.1111/j.1432-1033.1967.tb00110.x"
    }
  },
  "subtitle": [
    "Tryptic Peptides of Proteins by Diagonal Paper Electrophoresis A Carboxyl\u2010terminal Sequence for Pepsin"
  ],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1967,
        7
      ]
    ]
  },
  "references-count": 23,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1967,
          7
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1432-1033.1967.tb00110.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1432-1033.1967.tb00110.x",
  "relation": {},
  "ISSN": [
    "0014-2956",
    "1432-1033"
  ],
  "subject": [],
  "container-title-short": "European Journal of Biochemistry",
  "published": {
    "date-parts": [
      [
        1967,
        7
      ]
    ]
  }
}