DOI: 10.1111/j.1399-6576.1975.tb05407.x. Metabolic Changes in the Cerebral Cortex of the Rat Induced by Intravenous Pentothalsodium®

Metabolic Changes in the Cerebral Cortex of the Rat Induced by Intravenous Pentothalsodium®

10.1111/j.1399-6576.1975.tb05407.x

Crossref journal-article

Wiley

Acta Anaesthesiologica Scandinavica (311)

Abstract

The effects of thiopental on cerebral metabolism were evaluated by means of measurements of cerebral metabolic rate for oxygen (CMRo2) and of tissue levels of organic phosphates, glycolytic substrates, citric acid cycle intermediates and selected amino acids. Shortly after the beginning of thiopental administration, CMRo2 was reduced to half the normal value, and there were signs of retardation of glycolytic flux at the phosphofructokinase step. Inhibition at this regulatory enzymatic step could be related to an increase in phosphocreatine and a fall in inorganic phosphate concentration. The rise in phosphocreatine, and the unchanged levels of ATP, ADP and AMP demonstrate that induction of anaesthesia with thiopental is unrelated to energy failure.Changes in citric acid intermediates included a fall in malate, and probably also in citrate, α‐ketoglutarate and fumarate, with a tendency towards reduction in the pool of citric acid cycle intermediates; and the amino acid changes were dominated by a progressive rise in aspartate. It is suggested that these changes are secondary to a reduced rate of pyruvate delivery, and to a decrease in malate/oxaloacetate ratio.

Bibliography

Carlsson, G., Harp, J. R., & SiesjÃ¶, B. K. (1975). Metabolic Changes in the Cerebral Cortex of the Rat Induced by Intravenous PentothalsodiumÂ®. Acta Anaesthesiologica Scandinavica, 19(s57), 7â17. Portico.

Authors 3

Ghrister Carlsson (first)
James R. Harp (additional)
Bo K. Siesjö (additional)

References 35 Referenced 26

10.3109/00365517409049894
10.1038/1821190a0
10.1126/science.137.3529.499
10.1016/S0021-9258(19)84014-0 / J. biol. Chem. / Inhibition of electron and energy transfer in mitochondria. I. Effects of amytal, thiopental, rotenone, progesterone and methylene glycol. by Chance B. (1963)
10.1016/0304-4165(66)90114-0
10.1111/j.1471-4159.1972.tb01417.x
10.1111/j.1748-1716.1974.tb05651.x
{'issue': '3', 'key': 'e_1_2_1_9_1', 'first-page': '124', 'article-title': 'Alternative pathways of mitochondrial DPNH oxidation, studied with amytal', 'author': 'Ernster L.', 'year': '1955', 'journal-title': 'Exp. Cell. Res.'} / Exp. Cell. Res. / Alternative pathways of mitochondrial DPNH oxidation, studied with amytal by Ernster L. (1955)
10.1111/j.1471-4159.1972.tb01309.x
10.1111/j.1471-4159.1972.tb01310.x
10.1111/j.1471-4159.1974.tb04344.x
10.1016/0006-291X(64)90357-2
10.1016/S0021-9258(18)99802-9
{'key': 'e_1_2_1_15_1', 'volume-title': 'A Basis and Practice of Neuroanaesthesia', 'author': 'Harp J. R.', 'year': '1975'} / A Basis and Practice of Neuroanaesthesia by Harp J. R. (1975)
10.1042/bj0540082
{'key': 'e_1_2_1_17_1', 'first-page': '125', 'article-title': 'Control of metabolic processes.', 'volume': '16', 'author': 'Krebs H. A.', 'year': '1957', 'journal-title': 'Endeavour'} / Endeavour / Control of metabolic processes. by Krebs H. A. (1957)
10.1016/0006-291X(69)90253-8
10.1016/S0021-9258(18)51740-3
10.1016/S0021-9258(18)96616-0 / J. biol. Chem. / Kinetic evidence for multiple binding sites on phosphofructokinase. by Lowry O. H. (1966)
{'key': 'e_1_2_1_21_1', 'volume-title': 'A Flexible System of Enzymatic Analysis', 'author': 'Lowry O. H.', 'year': '1972'} / A Flexible System of Enzymatic Analysis by Lowry O. H. (1972)
10.1111/j.1471-4159.1973.tb00283.x
{'key': 'e_1_2_1_23_1', 'volume-title': 'Current Topics in Cellular Regulation', 'author': 'Mansour T. E.', 'year': '1972'} / Current Topics in Cellular Regulation by Mansour T. E. (1972)
{'key': 'e_1_2_1_24_1', 'first-page': '237', 'article-title': 'Regulation of enzyme activity.', 'volume': '53', 'author': 'Newsholme E. A.', 'year': '1965', 'journal-title': 'Sci. Progr.'} / Sci. Progr. / Regulation of enzyme activity. by Newsholme E. A. (1965)
10.1111/j.1748-1716.1970.tb04787.x
10.1111/j.1471-4159.1974.tb06912.x
10.1111/j.1748-1716.1974.tb05671.x
10.1016/0006-8993(75)90635-6
10.1016/0006-8993(75)90636-8
10.1111/j.1471-4159.1973.tb07567.x
10.1098/rspb.1932.0078
10.1042/bj1040524
10.1016/B978-0-12-152805-8.50008-3 / Current Topics in Cellular Regulation / A theoretical background to the use of measured concentrations of intermediates in study of the control of intermediary metabolism by Rolleston F. S. (1972)
10.1152/ajplegacy.1967.213.5.1143 / Amer. J. Physiol. / Effect of alkalosis on glycolysis in the isolated rat heart. by Scheuer J. (1967)
10.1111/j.1471-4159.1972.tb01308.x
10.1097/00000542-197204000-00015

Dates

Type	When
Created	16 years, 7 months ago (Jan. 1, 2009, 11:24 p.m.)
Deposited	1 year, 11 months ago (Sept. 26, 2023, 12:46 a.m.)
Indexed	12 hours, 9 minutes ago (Aug. 28, 2025, 8:38 a.m.)
Issued	50 years, 3 months ago (May 1, 1975)
Published	50 years, 3 months ago (May 1, 1975)
Published Online	16 years, 7 months ago (Dec. 31, 2008)
Published Print	50 years, 3 months ago (May 1, 1975)

Funders 0

None

BibTeX

@article{Carlsson_1975, title={Metabolic Changes in the Cerebral Cortex of the Rat Induced by Intravenous Pentothalsodium®}, volume={19}, ISSN={1399-6576}, url={http://dx.doi.org/10.1111/j.1399-6576.1975.tb05407.x}, DOI={10.1111/j.1399-6576.1975.tb05407.x}, number={s57}, journal={Acta Anaesthesiologica Scandinavica}, publisher={Wiley}, author={Carlsson, Ghrister and Harp, James R. and Siesjö, Bo K.}, year={1975}, month=may, pages={7–17} }

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