DOI: 10.1111/j.1399-3089.1996.tb00133.x. Human anti‐pig cell‐mediated cytotoxicity in vitro involves non‐T as well as T cell components

Human anti‐pig cell‐mediated cytotoxicity in vitro involves non‐T as well as T cell components

10.1111/j.1399-3089.1996.tb00133.x

Crossref journal-article

Wiley

Xenotransplantation (311)

Abstract

Abstract:Human anti‐pig cell‐mediated cytotoxicity was studied in vitro. Unprimed human peripheral blood lymphocytes (PBL) were able to lyse pig targets but not human targets when the assay was performed in human serum (HS). Much less, but still detectable, lysis was obtained using fetal calf serum (FCS). Human PBLs cultured for 6 days in FCS without stimulating cells showed substantial lysis of pig targets. This lysis was increased by the addition of human IL‐2. Unseparated human PBLs stimulated for 6 days with pig cells in serum‐free media lysed pig targets expressing the same or different SLA antigens as the stimulating cells. This lysis could not be significantly inhibited by anti‐CD3 or anti‐CD8 blocking antibodies during the effector phase of the assay. T cell‐enriched human PBLs treated with anti‐CD 16 and anti‐CD56 antibodies plus complement also lysed pig targets. These effector cells were significantly inhibited by anti‐CD3 and anti‐CD8 antibodies but not by anti‐CD4 antibodies. Furthermore, these primed T cell effectors could only lyse pig targets that shared the same MHC class I antigens as the sensitizing stimulators.These results suggest that human anti‐pig cell‐mediated cytotoxicity in vitro has at least three different components in bulk culture: 1) an ADCC component depending on natural antibodies from human serum, 2) an NK and/or a LAK cell component that is enriched by in vitro culture and interleukin‐2 (IL‐2), and (3) an allospecific T cell component, involving CD3+, CD8+, class I‐specific effector cells.

Bibliography

Chan, D. V., & Auchincloss, H. (1996). Human antiâpig cellâmediated cytotoxicity in vitro involves nonâT as well as T cell components. Xenotransplantation, 3(1), 158â165. Portico.

Authors 2

Derek V. Chan (first)
Hugh Auchincloss (additional)

References 20 Referenced 25

10.1097/00007890-199201000-00039
{'key': 'e_1_2_1_3_2', 'first-page': '2881', 'article-title': 'Xenogeneic cellular response of human lymphocytes to porcine lymphocytes and isolated porcine pancreatic islets', 'volume': '24', 'author': 'Heiser A', 'year': '1992', 'journal-title': 'Transplant Proc'} / Transplant Proc / Xenogeneic cellular response of human lymphocytes to porcine lymphocytes and isolated porcine pancreatic islets by Heiser A (1992)
10.1097/00007890-199304000-00043
10.1016/1074-7613(94)90009-4
10.1097/00007890-199457120-00004
10.1111/j.1399-0012.1993.tb00917.x / Clin Transplantation / Characterization of cellular human anti‐porcine xenoreactivity by Kumagai‐Braesch M (1993)
10.1097/00007890-199503270-00017
10.4049/jimmunol.155.11.5249 / J Immunol / Human anti‐porcine xenogeneic T cell response: Evidence for allelic specificity of mixed leukocyte reaction and for both direct and indirect pathways of recognition by Yamada K (1995)
10.1097/00007890-199511150-00025
{'key': 'e_1_2_1_11_2', 'first-page': '447', 'article-title': 'Role of activated natural killer and CD4+, CD8+ cells in the cellular rejection of a discordant xenograft', 'volume': '25', 'author': 'Elsen M', 'year': '1993', 'journal-title': 'Transplant Proc'} / Transplant Proc / Role of activated natural killer and CD4+, CD8+ cells in the cellular rejection of a discordant xenograft by Elsen M (1993)
10.1111/j.1600-065X.1994.tb00878.x
{'key': 'e_1_2_1_13_2', 'first-page': '1034', 'article-title': 'Humoral and cellular recognition of discordant xenogeneic Langerhans islets', 'volume': '26', 'author': 'Inverardi L', 'year': '1994', 'journal-title': 'Transplant Proc'} / Transplant Proc / Humoral and cellular recognition of discordant xenogeneic Langerhans islets by Inverardi L (1994)
10.1097/00007890-197612000-00004
10.1097/00007890-199405150-00016
10.1111/j.1399-0012.1993.tb00918.x / Clinical Transplantation / Characterization of humoral human anti‐porcine xenoreactivity by Satake M (1993)
10.1016/1074-7613(95)90079-9
10.1016/1074-7613(95)90025-X
10.1038/372150a0
10.1084/jem.181.5.1911
10.1016/1074-7613(95)90069-1

Dates

Type	When
Created	16 years, 9 months ago (Nov. 12, 2008, 11:29 a.m.)
Deposited	6 months, 3 weeks ago (Feb. 2, 2025, 1:17 p.m.)
Indexed	4 months, 3 weeks ago (March 31, 2025, 6:42 a.m.)
Issued	29 years, 6 months ago (Feb. 1, 1996)
Published	29 years, 6 months ago (Feb. 1, 1996)
Published Online	16 years, 9 months ago (Nov. 12, 2008)
Published Print	29 years, 6 months ago (Feb. 1, 1996)

Funders 0

None

BibTeX

@article{Chan_1996, title={Human anti‐pig cell‐mediated cytotoxicity in vitro involves non‐T as well as T cell components}, volume={3}, ISSN={1399-3089}, url={http://dx.doi.org/10.1111/j.1399-3089.1996.tb00133.x}, DOI={10.1111/j.1399-3089.1996.tb00133.x}, number={1}, journal={Xenotransplantation}, publisher={Wiley}, author={Chan, Derek V. and Auchincloss, Hugh}, year={1996}, month=feb, pages={158–165} }

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