DOI: 10.1111/j.1399-3054.1990.tb02085.x. Are‐examination of NaCI effects on phosphoenolpyruvate carboxylase at high (physiological) enzyme concentrations

Are‐examination of NaCI effects on phosphoenolpyruvate carboxylase at high (physiological) enzyme concentrations

10.1111/j.1399-3054.1990.tb02085.x

Crossref journal-article

Wiley

Physiologia Plantarum (311)

Abstract

Inhibition of phosphoenolpyruvate carboxylase (EC 4.1.1 31) from the C4‐halophyte Salsola soda L. by NaCI is compeutive to phosphoenolpyruvate (PEP). Physiological (betaine, glycerol) and synthetic (polyethylene glycol) osmotica and the allosteric activator glucose‐6‐phosphate (G6P) increase the apparent affinity of the enzyme for PEP and also alleviate the inhibition by NaCl. Physiological osmotica that either increase the Km(PEP) (proline) or are neutral (sorbitol), do not protect the enzyme against NaCI attack. In the absence of cosolutes and, G6P, the enzyme is self‐protected when its concentration in the assay medium is increased to more physiological values. In addition, the amount of betaine needed for complete protection is inversely related to native protein concentration in the assay. Exogenous protein (bovine serum albumin or bovine skin gelatin) have no effect on either Km(PEP), or extent of NaCl inhibition. These results can be better explained with the exclusion volume theory and the inferred assumption that both cosolutes and high protein concentration strengthen intrinsic aggregation properties of enzymes. It is suggested that the extremely high phosphoenolpyruvate carboxylase concentration in the cytoplasm and the accumulation of compatible solutes in response to water stress fully protect the enzyme in vivo against the chaotropic effects of NaCI.

Bibliography

Manetas, Y. (1990). Areâexamination of NaCI effects on phosphoenolpyruvate carboxylase at high (physiological) enzyme concentrations. Physiologia Plantarum, 78(2), 225â229. Portico.

Authors 1

Yiannis Manetas (first)

References 34 Referenced 34

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{'key': 'e_1_2_2_7_1', 'first-page': '287', 'article-title': 'Osmoregulation in halophytic higher plants: a comparative study of soluble carbohydrates, polyols, betaines and free proline', 'volume': '5', 'author': 'Briens M.', 'year': '1982', 'journal-title': 'Plant Cell Environ.'} / Plant Cell Environ. / Osmoregulation in halophytic higher plants: a comparative study of soluble carbohydrates, polyols, betaines and free proline by Briens M. (1982)
10.1146/annurev.pp.28.060177.000513
10.1093/oxfordjournals.aob.a085546 / Ann. Bot. / Salt tolerance in the halophyte Sueaeda maritima (L.) Dum: properties of malic enzyme and PEP carboxylase by Flowers T. J. (1978)
{'key': 'e_1_2_2_10_1', 'first-page': '49', 'article-title': 'Factors affecting a fast and reversible inactivation of photosynthetic phosphoenolpyruvate earhoxylase', 'volume': '16', 'author': 'Gavalas N. A.', 'year': '1982', 'journal-title': 'Photosynthetica'} / Photosynthetica / Factors affecting a fast and reversible inactivation of photosynthetic phosphoenolpyruvate earhoxylase by Gavalas N. A. (1982)
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{'key': 'e_1_2_2_22_1', 'first-page': '107', 'article-title': 'Proline, betaine and other organic solutes protect enzymes against heat inactivation', 'volume': '8', 'author': 'Paleg L. G.', 'year': '1981', 'journal-title': 'Aust. J. Plant Physiol.'} / Aust. J. Plant Physiol. / Proline, betaine and other organic solutes protect enzymes against heat inactivation by Paleg L. G. (1981)
{'key': 'e_1_2_2_23_1', 'first-page': '190', 'article-title': 'Maize leaf phosphoenol pyruvate carboxylase quaternary structure is stabilized by glycerol', 'volume': '89', 'author': 'Podesta F. C.', 'year': '1989', 'journal-title': 'Plant. Physiol.'} / Plant. Physiol. / Maize leaf phosphoenol pyruvate carboxylase quaternary structure is stabilized by glycerol by Podesta F. C. (1989)
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Dates

Type	When
Created	19 years, 4 months ago (April 30, 2006, 10:10 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 4:30 p.m.)
Indexed	4 weeks, 1 day ago (Aug. 7, 2025, 4:55 a.m.)
Issued	35 years, 7 months ago (Feb. 1, 1990)
Published	35 years, 7 months ago (Feb. 1, 1990)
Published Online	19 years, 4 months ago (April 28, 2006)
Published Print	35 years, 7 months ago (Feb. 1, 1990)

Funders 0

None

BibTeX

@article{Manetas_1990, title={Are‐examination of NaCI effects on phosphoenolpyruvate carboxylase at high (physiological) enzyme concentrations}, volume={78}, ISSN={1399-3054}, url={http://dx.doi.org/10.1111/j.1399-3054.1990.tb02085.x}, DOI={10.1111/j.1399-3054.1990.tb02085.x}, number={2}, journal={Physiologia Plantarum}, publisher={Wiley}, author={Manetas, Yiannis}, year={1990}, month=feb, pages={225–229} }

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