DOI: 10.1111/j.1399-3054.1989.tb05660.x. Hydrophobicity of protochlorophyllide oxidoreductase, characterized by means of Triton X‐114 partitioning of isolated etioplast membrane fractions

Hydrophobicity of protochlorophyllide oxidoreductase, characterized by means of Triton X‐114 partitioning of isolated etioplast membrane fractions

10.1111/j.1399-3054.1989.tb05660.x

Crossref journal-article

Wiley

Physiologia Plantarum (311)

Abstract

The inner etioplast membrane possesses a pronounced lateral heterogeneity with respect to protein and lipid composition as well as ultrastructural appearance. Little is known about the reason for formation of the regular branched structure shown by the prolamellar body part of the membrane. A specific interaction between the membrane lipids and the dominating protein NADPH‐protochlorophyllide oxidoreductase (PCR, EC 1.6.99.1) might be of major importance. In this study isolated prolamellar bodies and prothylakoids from the leaves of dark‐grown wheat (Triticum aestivum L. cv. Starke II, Weibull) were exposed to Triton X‐114 partitioning in media with 150 mM NaCl and without. By comparing the partitioning of PCR, the ATP synthase (EC 3.6.1.3) polypeptides, and ribulosebisphosphate carboxylaseoxygenase (EC 4.1.1.39) in the different systems, it was concluded that PCR is an integral membrane protein with substantial hydrophilic domains.

Bibliography

Selstam, E., & WidellâWigge, A. (1989). Hydrophobicity of protochlorophyllide oxidoreductase, characterized by means of Triton Xâ114 partitioning of isolated etioplast membrane fractions. Physiologia Plantarum, 77(3), 401â406. Portico.

Authors 2

Eva Selstam (first)
Anna Widell‐Wigge (additional)

References 26 Referenced 24

10.1016/S0021-9258(19)69848-0
10.1016/0005-2736(85)90277-9
10.1016/0014-5793(82)81100-9
10.1016/0014-5793(87)80309-5
10.1042/bj1740681
{'key': 'e_1_2_2_7_1', 'first-page': '245', 'volume-title': 'Chloroplast Biogenesis', 'author': 'Griffiths W. T.', 'year': '1984'} / Chloroplast Biogenesis by Griffiths W. T. (1984)
10.1093/oxfordjournals.pcp.a076515
10.1111/j.1399-3054.1988.tb06639.x
10.1016/0003-9861(81)90138-7
10.1146/annurev.bi.52.070183.002451
10.1016/B978-0-12-152511-8.50008-6
10.1016/0968-0004(86)90132-5
10.1042/bj2330525
10.1016/0168-9452(87)90099-9
10.1111/j.1399-3054.1986.tb05589.x
10.1111/j.1399-3054.1982.tb00313.x
10.1111/j.1399-3054.1983.tb02785.x
10.1104/pp.76.4.1041
10.1104/pp.76.4.1036
10.1111/j.1399-3054.1986.tb05746.x
{'key': 'e_1_2_2_22_1', 'first-page': '50', 'article-title': 'Immunogold localisation of protochlorophyllide oxidoreductase in barley etioplasts', 'volume': '39', 'author': 'Shaw P.', 'year': '1985', 'journal-title': 'Eur. J. Cell Biol.'} / Eur. J. Cell Biol. / Immunogold localisation of protochlorophyllide oxidoreductase in barley etioplasts by Shaw P. (1985)
10.1016/0005-2728(73)90135-7
10.1016/0005-2728(80)90164-4
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10.1016/S0074-7696(08)60369-1
{'key': 'e_1_2_2_27_1', 'first-page': '19A', 'article-title': 'Ionic effects on the prolamellar body structure and protochlorophyllide oxidoreductase', 'volume': '73', 'author': 'Widell A.', 'year': '1988', 'journal-title': 'Physiol. Plant.'} / Physiol. Plant. / Ionic effects on the prolamellar body structure and protochlorophyllide oxidoreductase by Widell A. (1988)

Dates

Type	When
Created	19 years, 4 months ago (April 30, 2006, 10:04 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 7:45 a.m.)
Indexed	3 weeks, 6 days ago (Aug. 5, 2025, 8:10 a.m.)
Issued	35 years, 10 months ago (Nov. 1, 1989)
Published	35 years, 10 months ago (Nov. 1, 1989)
Published Online	19 years, 4 months ago (April 28, 2006)
Published Print	35 years, 10 months ago (Nov. 1, 1989)

Funders 0

None

BibTeX

@article{Selstam_1989, title={Hydrophobicity of protochlorophyllide oxidoreductase, characterized by means of Triton X‐114 partitioning of isolated etioplast membrane fractions}, volume={77}, ISSN={1399-3054}, url={http://dx.doi.org/10.1111/j.1399-3054.1989.tb05660.x}, DOI={10.1111/j.1399-3054.1989.tb05660.x}, number={3}, journal={Physiologia Plantarum}, publisher={Wiley}, author={Selstam, Eva and Widell‐Wigge, Anna}, year={1989}, month=nov, pages={401–406} }

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