DOI: 10.1111/j.1399-3054.1985.tb02352.x. Glycosylation is not needed for the intracellular transport of phytohemagglutinin in developing <i>Phaseolus vulgaris</i> cotyledons and for the maintenance of its biological activities

Glycosylation is not needed for the intracellular transport of phytohemagglutinin in developing Phaseolus vulgaris cotyledons and for the maintenance of its biological activities

10.1111/j.1399-3054.1985.tb02352.x

Crossref journal-article

Wiley

Physiologia Plantarum (311)

Abstract

Approximately 10% of the total protein contained in Phaseolus vulgaris L. cv. Greensleeves seeds is composed of the glycoprotein lectin, phytohemagglutinin. We have investigated whether the presence of N‐linked oligosaccharide side chains is a prerequisite for the correct intracellular transport of this protein and whether unglycosylated phytohemagglutinin maintains its biological activities. Excised developing cotyledons were incubated in the presence of tunicamycin to prevent glycosylation “in vivo”, and the fate of the unglycosylated protein synthesized in such cotyledons determined. It was found that unglycosylated phytohemagglutinin reaches its normal site of accumulation, the protein bodies, and maintains erythro‐agglutinating and mitogenic activities.

Bibliography

Bollini, R., Ceriotti, A., Daminati, M. G., & Vitale, A. (1985). Glycosylation is not needed for the intracellular transport of phytohemagglutinin in developing Phaseolus vulgaris cotyledons and for the maintenance of its biological activities. Physiologia Plantarum, 65(1), 15â22. Portico.

Authors 4

Roberto Bollini (first)
Aldo Ceriotti (additional)
M. Gloria Daminati (additional)
Alessandro Vitale (additional)

References 31 Referenced 47

{'key': 'e_1_2_2_2_1', 'first-page': '313', 'volume-title': 'Methods in Enzymology', 'author': 'Agrawal B. B. L.', 'year': '1972'} / Methods in Enzymology by Agrawal B. B. L. (1972)
10.1111/j.1432-1033.1983.tb07465.x
10.1016/0304-4165(78)90212-X
10.1007/BF00385080
10.1007/BF00380865
10.1111/j.1399-3054.1981.tb06040.x
10.1111/j.1399-3054.1982.tb02268.x
10.1083/jcb.96.4.999
10.1111/j.1432-1033.1974.tb03599.x
{'issue': '97', 'key': 'e_1_2_2_11_1', 'first-page': '77', 'article-title': 'Separation of lekocytes from blood and bone marrow', 'volume': '21', 'author': 'Boyum A.', 'year': '1968', 'journal-title': 'Scand. J. Clin. Lab. Invest.'} / Scand. J. Clin. Lab. Invest. / Separation of lekocytes from blood and bone marrow by Boyum A. (1968)
{'key': 'e_1_2_2_12_1', 'first-page': '517', 'article-title': 'Differenza nel comportamento delle popolazioni T‐linfocitarie nei dipendenti da eroina e da metadone', 'volume': '62', 'author': 'Brugo M. A.', 'year': '1983', 'journal-title': 'Boll. Ist. Sieroter. Milan.'} / Boll. Ist. Sieroter. Milan. / Differenza nel comportamento delle popolazioni T‐linfocitarie nei dipendenti da eroina e da metadone by Brugo M. A. (1983)
10.1042/bj1770509
10.1007/BF00397707
10.1104/pp.70.5.1425
10.1016/0005-2795(75)90316-5
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10.1007/BF00033381
10.1016/S0006-291X(73)80042-7
10.1016/S0021-9258(19)41790-0 / J. Biol. Chem. / Labeling of soybean agglutinin by oxidation with sodium periodate followed by reduction with sodium [3H]‐borohydride by Lotan R. (1975)
10.1016/S0021-9258(19)52451-6
{'key': 'e_1_2_2_22_1', 'first-page': '255', 'volume-title': 'Encyclopedia of Plant Physiology, New Series', 'author': 'Matile, Ph', 'year': '1976'} / Encyclopedia of Plant Physiology, New Series by Matile, Ph (1976)
10.1071/PP9750051
10.1073/pnas.76.7.3343
10.1016/0304-4157(82)90017-X
10.1016/S0021-9258(19)45337-4 / J. Biol. Chem. / Glycosylation and intracellular transport of membrane glycoproteins encoded by murine leukemia viruses by Polonoff E. (1982)
10.1002/jcb.1982.240180107
10.1016/S0006-291X(75)80086-6
10.1083/jcb.99.1.133
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10.1007/BF00402863
10.1016/S0021-9258(17)40794-0 / J. Biol. Chem. / Binding and functional properties of concanavalin A and its derivatives by Wang J. L. (1978)

Dates

Type	When
Created	19 years, 3 months ago (April 30, 2006, 7:18 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 9:24 a.m.)
Indexed	2 weeks, 3 days ago (Aug. 7, 2025, 4:55 a.m.)
Issued	39 years, 11 months ago (Sept. 1, 1985)
Published	39 years, 11 months ago (Sept. 1, 1985)
Published Online	19 years, 3 months ago (April 28, 2006)
Published Print	39 years, 11 months ago (Sept. 1, 1985)

Funders 0

None

BibTeX

@article{Bollini_1985, title={Glycosylation is not needed for the intracellular transport of phytohemagglutinin in developing Phaseolus vulgaris cotyledons and for the maintenance of its biological activities}, volume={65}, ISSN={1399-3054}, url={http://dx.doi.org/10.1111/j.1399-3054.1985.tb02352.x}, DOI={10.1111/j.1399-3054.1985.tb02352.x}, number={1}, journal={Physiologia Plantarum}, publisher={Wiley}, author={Bollini, Roberto and Ceriotti, Aldo and Daminati, M. Gloria and Vitale, Alessandro}, year={1985}, month=sep, pages={15–22} }

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