DOI: 10.1111/j.1399-3054.1983.tb04220.x. Synchrony induced by double phosphate starvation in a suspension culture of <i>Catharanthus roseus</i>

Synchrony induced by double phosphate starvation in a suspension culture of Catharanthus roseus

10.1111/j.1399-3054.1983.tb04220.x

Crossref journal-article

Wiley

Physiologia Plantarum (311)

Abstract

A synchronous cell division system was established using the double phosphate starvation method, based on the observation that one of the limiting factors in the growth of a suspension culture of Catharanthus roseus (L.) G. Don cells in the medium of Murashige and Skoog was phosphate. In the system, an increase in cell number took place in a short period of only 4 h, while the cell number remained almost constant during other periods of the cell cycle. The synchrony of the culture was confirmed by changes in mitotic index, which increased sharply prior to the increase in cell number. The S phase was determined by measuring incorporation of [3H]‐thymidine into the DNA fraction during the cell cycle and synchrony of DNA synthesis was verified likewise. Synchronization by phosphate starvation is discussed in relation to the function of phosphate as a nutrient. The synchronous system thus established will be useful in biochemical studies of the cell cycle in higher plants.

Bibliography

Amino, S., Fujimura, T., & Komamine, A. (1983). Synchrony induced by double phosphate starvation in a suspension culture of Catharanthus roseus. Physiologia Plantarum, 59(3), 393â396. Portico.

Authors 3

Shin‐ichi Amino (first)
Tatsuhito Fujimura (additional)
Atsushi Komamine (additional)

References 16 Referenced 85

Amino S.&Komamine A.1982.Dynamic aspects of cell walls during the cell cycle in a synchronous culture ofVinca rosea. – InPlanta Tissue Culture(A.Fujiwara ed.) pp.65–66. Japanese Association for Plant Tissue Culture.
10.1016/0014-4827(77)90124-0
10.1016/0304-4211(80)90135-2
10.1111/j.1399-3054.1966.tb07080.x
10.1016/0003-2697(75)90379-6
10.1016/0014-4827(68)90403-5
10.1242/jcs.17.3.337 / J. Cell Sci. / Kinetic aspects of synchrony in suspension cultures of Acer psettdoplatanus L by Gould A. R. (1975)
10.1007/BF02115957
10.1016/0014-4827(73)90565-X
{'key': 'e_1_2_2_11_1', 'first-page': '1', 'article-title': 'Plant tissue culture and the cell cycle', 'volume': '18', 'author': 'King P. J.', 'year': '1980', 'journal-title': 'Adv Biol. Eng.'} / Adv Biol. Eng. / Plant tissue culture and the cell cycle by King P. J. (1980)
{'key': 'e_1_2_2_12_1', 'first-page': '307', 'volume-title': 'Plant Tissue Culture', 'author': 'King P. J.', 'year': '1977'} / Plant Tissue Culture by King P. J. (1977)
10.1007/BF00390793
10.1111/j.1399-3054.1962.tb08052.x
10.1016/0014-4827(73)90094-3
10.1007/BF02506252
10.1093/oxfordjournals.aob.a084142

Dates

Type	When
Created	19 years, 4 months ago (April 30, 2006, 6:04 p.m.)
Deposited	1 year, 10 months ago (Oct. 19, 2023, 3:18 p.m.)
Indexed	1 month ago (Aug. 5, 2025, 8:10 a.m.)
Issued	41 years, 10 months ago (Nov. 1, 1983)
Published	41 years, 10 months ago (Nov. 1, 1983)
Published Online	19 years, 4 months ago (April 28, 2006)
Published Print	41 years, 10 months ago (Nov. 1, 1983)

Funders 0

None

BibTeX

@article{Amino_1983, title={Synchrony induced by double phosphate starvation in a suspension culture of Catharanthus roseus}, volume={59}, ISSN={1399-3054}, url={http://dx.doi.org/10.1111/j.1399-3054.1983.tb04220.x}, DOI={10.1111/j.1399-3054.1983.tb04220.x}, number={3}, journal={Physiologia Plantarum}, publisher={Wiley}, author={Amino, Shin‐ichi and Fujimura, Tatsuhito and Komamine, Atsushi}, year={1983}, month=nov, pages={393–396} }

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