DOI: 10.1111/j.1399-3054.1981.tb02698.x. Release of membrane proteins in relation to heat injury of spinach chloroplasts

Release of membrane proteins in relation to heat injury of spinach chloroplasts

10.1111/j.1399-3054.1981.tb02698.x

Crossref journal-article

Wiley

Physiologia Plantarum (311)

Abstract

Thylakoids isolated from spinach leaves (Spinacia oleracea L. cvs. Monatol and Montako) were exposed to supraoptimal temperatures that inactivated their photochemical reactions. Membrane injury was accompanied by release of a small amount of membrane proteins. When sucrose was present during high‐temperature treatment, thylakoids were partially protected and release of membrane proteins was less pronounced than in the absence of sugar. From thylakoids, which were isolated from heat‐damaged spinach leaves, less protein was released when heated again after the isolation procedure, indicating that protein release also takes place during heat inactivation in vivo. Sodium dodecyl sulfate gel electropherograms of thylakoids demonstrated that heat inactivation of the lamellae was not accompanied by significant changes in the pattern of the proteins, which remained in the membranes. The same was found when thylakoids were solubilized with Triton X‐100 before and after heat damage. It is suggested that the protein release that occurs during heat treatment is a consequence of irreversible alterations in the membrane structure; these changes may be responsible for thermal damage of chloroplast membranes.

Bibliography

Volger, H., & Santarius, K. A. (1981). Release of membrane proteins in relation to heat injury of spinach chloroplasts. Physiologia Plantarum, 51(2), 195â200. Portico.

Authors 2

Henning Volger (first)
Kurt A. Santarius (additional)

References 28 Referenced 26

{'key': 'e_1_2_2_2_1', 'volume-title': 'Ecological Studies', 'author': 'Alexandrov V. Y.', 'year': '1977'} / Ecological Studies by Alexandrov V. Y. (1977)
10.1104/pp.24.1.1
{'key': 'e_1_2_2_4_1', 'first-page': '751', 'article-title': 'Mechanistic studies of thermal damage to leaves', 'volume': '74', 'author': 'Berry J. A.', 'year': '1975', 'journal-title': 'Carnegie Inst. Year Book'} / Carnegie Inst. Year Book / Mechanistic studies of thermal damage to leaves by Berry J. A. (1975)
{'key': 'e_1_2_2_5_1', 'first-page': '748', 'article-title': 'Thermal stability of the photosynthetic apparatus in intact leaves', 'volume': '71', 'author': 'Björkman O.', 'year': '1975', 'journal-title': 'Ibid.'} / Ibid. / Thermal stability of the photosynthetic apparatus in intact leaves by Björkman O. (1975)
10.1073/pnas.72.6.2175
10.1083/jcb.67.2.361
{'key': 'e_1_2_2_8_1', 'first-page': '427', 'article-title': 'The relationship between changes of the physical phase of membrane lipids and photosynthesis in the thermophilic alga Cyanidium caldarium', 'author': 'Fork D. C.', 'year': '1977', 'journal-title': 'Photosynthetic Organelles. Special Issue of Plant Cell Physiol.'} / Photosynthetic Organelles. Special Issue of Plant Cell Physiol. / The relationship between changes of the physical phase of membrane lipids and photosynthesis in the thermophilic alga Cyanidium caldarium by Fork D. C. (1977)
10.1104/pp.57.5.673
{'key': 'e_1_2_2_10_1', 'first-page': '232', 'volume-title': 'Temperature and Life', 'author': 'Heber U.', 'year': '1973'} / Temperature and Life by Heber U. (1973)
10.1021/ba-1979-0180.ch008
10.1007/BF00380726
10.1038/227680a0
{'key': 'e_1_2_2_14_1', 'first-page': '231', 'volume-title': 'Thermobiology', 'author': 'Langridge J.', 'year': '1967'} / Thermobiology by Langridge J. (1967)
{'key': 'e_1_2_2_15_1', 'volume-title': 'Responses of Plants to Environmental Stresses', 'author': 'Levitt J.', 'year': '1972'} / Responses of Plants to Environmental Stresses by Levitt J. (1972)
{'key': 'e_1_2_2_16_1', 'first-page': '961', 'article-title': 'Hydrolysis of phospholipids and formation of free fatty acids in isolated chloroplasts', 'volume': '33', 'author': 'Molotkovsky Y. G.', 'year': '1968', 'journal-title': 'Biochimija'} / Biochimija / Hydrolysis of phospholipids and formation of free fatty acids in isolated chloroplasts by Molotkovsky Y. G. (1968)
10.1104/pp.56.4.508
10.1007/BF00388196
10.1016/0306-4565(76)90028-0
10.1111/j.1399-3054.1980.tb08638.x
10.1007/BF00388828
10.1111/j.1438-8677.1979.tb03273.x
10.1016/S0021-9258(19)73780-6 / J. Biol. Chem. / The contribution of hydrophobic bonds to the thermal stability of protein conformations by Scheraga H. A. (1962)
10.1016/0005-2728(78)90138-X
10.1007/BF00385990
10.1016/0011-2240(77)90178-X
10.1111/j.1749-6632.1972.tb54779.x
10.1016/0005-2736(78)90281-X
10.1016/S0015-3796(17)30160-9 / Biochem. Physiol. Pflanzen / Influence of post‐action of high temperature on photosynthetic activity, composition of lamellar proteins and spectral characteristics of pigment systems I and II by Yordanov I. (1975)

Dates

Type	When
Created	19 years, 3 months ago (April 30, 2006, 5:35 p.m.)
Deposited	1 year, 9 months ago (Nov. 10, 2023, 7:15 p.m.)
Indexed	2 weeks, 6 days ago (Aug. 7, 2025, 5:02 p.m.)
Issued	44 years, 6 months ago (Feb. 1, 1981)
Published	44 years, 6 months ago (Feb. 1, 1981)
Published Online	19 years, 4 months ago (April 28, 2006)
Published Print	44 years, 6 months ago (Feb. 1, 1981)

Funders 0

None

BibTeX

@article{Volger_1981, title={Release of membrane proteins in relation to heat injury of spinach chloroplasts}, volume={51}, ISSN={1399-3054}, url={http://dx.doi.org/10.1111/j.1399-3054.1981.tb02698.x}, DOI={10.1111/j.1399-3054.1981.tb02698.x}, number={2}, journal={Physiologia Plantarum}, publisher={Wiley}, author={Volger, Henning and Santarius, Kurt A.}, year={1981}, month=feb, pages={195–200} }

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