DOI: 10.1111/j.1399-302x.1993.tb00557.x. Effect of outer membrane of <i>Treponema denticola</i> on bone resorption

Effect of outer membrane of Treponema denticola on bone resorption

10.1111/j.1399-302x.1993.tb00557.x

Crossref journal-article

Wiley

Oral Microbiology and Immunology (311)

Abstract

The effect of the outer membrane (outer sheath) of Treponema denticola on bone resorption was studied. Bone resorption was measured by the release of previously incorporated 45Ca from the shafts of the radii and ulnae of 19‐day fetal rats. A treated‐over‐control ratio (T/C ratio) significantly greater than 1 indicated the stimulation of bone resorption by the test substance. The addition of outer membrane of T. denticola increased the release of 45Ca from the assay bones. The minimum concentrations required to yield significant 45Ca release from the assay bones were 15, 22 and 75 μg protein/ml for serovars a, b and c, respectively. These protein values corresponded to estimated lipopolysaccharide contents of 0.6, 0.8 and 2.8 μg/ml, based on 3‐deoxy‐2‐manno‐octulosonate analysis. Heat treatment of outer membrane (60° for 30 min) did not change the effect on 45Ca release. Parathyroid hormone or prostaglandin E2 known to act synergistically with lipopolysaccharides in bone resorption, was also added to the assay system. Neither prostaglandin E2 at 10‐7 M nor parathyroid hormone at 40 ng/ml, by itself, increased 45Ca release. However, in the presence of 10 μg protein/ml of outer membrane of serovar b at 120 h, the T/C ratio was increased to 1.31±0.07 and 1.58±0.118, respectively. These results suggest that a lipopolysaccharide‐like material is present in the outer membrane of T. denticola that may be responsible for bone resorption in the in vitro system.

Bibliography

Gopalsami, C., Yotis, W., Corrigan, K., Schade, S., Keene, J., & Simonson, L. (1993). Effect of outer membrane of Treponema denticola on bone resorption. Oral Microbiology and Immunology, 8(2), 121â124. Portico.

Authors 6

C. Gopalsami (first)
W. Yotis (additional)
K. Corrigan (additional)
S. Schade (additional)
J. Keene (additional)
L. Simonson (additional)

References 23 Referenced 17

10.1902/jop.1982.53.9.550
10.1128/JB.128.2.616-622.1976
10.1111/j.1600-0765.1978.tb00177.x
{'key': 'e_1_2_1_5_2', 'first-page': '126', 'volume-title': 'Osteoporosis', 'author': 'Hausmann E', 'year': '1982'} / Osteoporosis by Hausmann E (1982)
{'key': 'e_1_2_1_6_2', 'first-page': '151', 'volume-title': 'Host‐parasite interactions in periodontal diseases', 'author': 'Hausmann E', 'year': '1982'} / Host‐parasite interactions in periodontal diseases by Hausmann E (1982)
10.1126/science.168.3933.862
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10.1016/0003-2697(88)90109-1
{'key': 'e_1_2_1_10_2', 'first-page': '307', 'volume-title': 'Periodontal disease: pathogens and host immune responses', 'author': 'Hopps RM', 'year': '1991'} / Periodontal disease: pathogens and host immune responses by Hopps RM (1991)
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{'key': 'e_1_2_1_21_2', 'first-page': '318', 'article-title': 'Mitogenic activity in outer membrane from Treponema denticola', 'volume': '71', 'author': 'Schade S', 'year': '1992', 'journal-title': 'J Dent Res'} / J Dent Res / Mitogenic activity in outer membrane from Treponema denticola by Schade S (1992)
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10.1128/jcm.29.7.1397-1406.1991 / J Clin Microbiol / Biochemical properties of the outer membrane of Treponema denticola by Yotis WW (1991)

Dates

Type	When
Created	17 years, 8 months ago (Dec. 19, 2007, 3:12 a.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 12:42 a.m.)
Indexed	1 year, 10 months ago (Oct. 28, 2023, 5:18 a.m.)
Issued	32 years, 5 months ago (April 1, 1993)
Published	32 years, 5 months ago (April 1, 1993)
Published Online	17 years, 8 months ago (Dec. 19, 2007)
Published Print	32 years, 5 months ago (April 1, 1993)

Funders 0

None

BibTeX

@article{Gopalsami_1993, title={Effect of outer membrane of Treponema denticola on bone resorption}, volume={8}, ISSN={1399-302X}, url={http://dx.doi.org/10.1111/j.1399-302x.1993.tb00557.x}, DOI={10.1111/j.1399-302x.1993.tb00557.x}, number={2}, journal={Oral Microbiology and Immunology}, publisher={Wiley}, author={Gopalsami, C. and Yotis, W. and Corrigan, K. and Schade, S. and Keene, J. and Simonson, L.}, year={1993}, month=apr, pages={121–124} }

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