DOI: 10.1111/j.1399-3011.1996.tb00813.x. Dynorphin‐phospholipid membrane interactions: Role of phospholipid head‐group and cholesterol

Dynorphin‐phospholipid membrane interactions: Role of phospholipid head‐group and cholesterol

10.1111/j.1399-3011.1996.tb00813.x

Crossref journal-article

Wiley

International Journal of Peptide and Protein Research (311)

Abstract

The interaction of the κ‐opioid receptor‐selective heptadecapeptide dynorphin A(1‐17) (Tyr1‐Gly‐Gly‐Phe‐Leu5‐Arg‐Arg‐Ile‐Arg‐Pro10‐Lys‐Leu‐Lys‐Trp‐Asp15‐Asn‐Glu) with phospholipid membranes has been investigated by monitoring the leakage of the internal aqueous contents of liposomes, the changes in the tryptophan emission spectrum, and the collisional quenching of tryptophan fluorescence by brominated lipids. The peptide induces more extensive leakage of contents from phosphatidylserine than from phosphatidyl‐choline vesicles, and experiences a blue shift of the Trp fluorescence emission maximum in the presence of phosphatidylserine vesicles. In the presence of phosphatidylcholine vesicles, however, the Trp fluorescence intensity is reduced without a blue shift. In phosphatidylserine membranes containing 10 mol% phosphatidylcholine, the intensity of the blue‐shifted fluorescence is enhanced. This avid interaction of dynorphin A(1‐17) with phosphatidylserine membranes is likely to be mediated by the positively charged Arg and Lys groups. It is proposed that. while the N‐terminus of the peptide may be embedded in the bilayer in analogy with dynorphin (1–13), the C‐terminal region of dynorphin A (1‐17) bends back onto the bilayer/water interphase, and that the Trp14, residue is stabilized in a hydrophobic pocked near the interphase by the interaction of the neighboring charged amino acids with the phosphate, carboxyl and amino groups on phosphatidylserine. ©Munksgaard 1996.

Bibliography

ALFORD, D. R., RENUGOPALAKRISHNAN, V., & DÃZGÃNES, N. (1996). Dynorphinâphospholipid membrane interactions: Role of phospholipid headâgroup and cholesterol. International Journal of Peptide and Protein Research, 47(1â2), 84â90. Portico.

Authors 3

DENNIS R. ALFORD (first)
V. RENUGOPALAKRISHNAN (additional)
NEJAT DÜZGÜNES (additional)

References 30 Referenced 8

10.1073/pnas.76.12.6666
10.1073/pnas.78.11.7219
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{'key': 'e_1_2_1_4_2', 'first-page': '363', 'volume-title': 'Pharmacology', 'author': 'Theoharides T.C.', 'year': '1992'} / Pharmacology by Theoharides T.C. (1992)
{'key': 'e_1_2_1_5_2', 'first-page': '284', 'volume': '30', 'author': 'Rapaka R.S.', 'year': '1987', 'journal-title': 'Int. J. Peptide Protein Res.'} / Int. J. Peptide Protein Res. by Rapaka R.S. (1987)
10.1016/S0006-291X(88)80496-0
Renugopalakrishnan V. Huang S.‐G.&Prabhakaran M.(1995)Int. J. Peptide Protein Res.(submitted).
10.1111/j.1399-3011.1983.tb03109.x
{'key': 'e_1_2_1_9_2', 'first-page': '61', 'volume-title': 'New Methods in Drug Research', 'author': 'Renugopalakrishnan V.', 'year': '1989'} / New Methods in Drug Research by Renugopalakrishnan V. (1989)
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Dates

Type	When
Created	15 years ago (Aug. 9, 2010, 5:31 p.m.)
Deposited	1 year, 11 months ago (Sept. 1, 2023, 2:10 p.m.)
Indexed	4 weeks ago (Aug. 2, 2025, 12:31 a.m.)
Issued	29 years, 7 months ago (Jan. 1, 1996)
Published	29 years, 7 months ago (Jan. 1, 1996)
Published Online	16 years, 7 months ago (Jan. 22, 2009)
Published Print	29 years, 7 months ago (Jan. 1, 1996)

Funders 0

None

BibTeX

@article{ALFORD_1996, title={Dynorphin‐phospholipid membrane interactions: Role of phospholipid head‐group and cholesterol}, volume={47}, ISSN={0367-8377}, url={http://dx.doi.org/10.1111/j.1399-3011.1996.tb00813.x}, DOI={10.1111/j.1399-3011.1996.tb00813.x}, number={1–2}, journal={International Journal of Peptide and Protein Research}, publisher={Wiley}, author={ALFORD, DENNIS R. and RENUGOPALAKRISHNAN, V. and DÜZGÜNES, NEJAT}, year={1996}, month=jan, pages={84–90} }

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