DOI: 10.1111/j.1399-3011.1993.tb00121.x. Comparative study of the stability of the folding intermediates of the calcium‐binding lysozymes

Comparative study of the stability of the folding intermediates of the calcium‐binding lysozymes

10.1111/j.1399-3011.1993.tb00121.x

Crossref journal-article

Wiley

International Journal of Peptide and Protein Research (311)

Abstract

Unfolding profiles of two calcium‐binding lysozymes, equine milk lysozyme and pigeon egg‐white lysozyme, were obtained by circular dichroism and proton NMR measurements. Equine lysozyme unfolds through a stable molten globule intermediate. The molten globule of equine lysozyme was characterized as more ordered than that of bovine α‐lactalbumin. On the other hand, pigeon lysozyme unfolds by a two‐state mechanism and the intermediate could not be observed in guanidine or thermal unfolding, the same as with conventional non‐calcium‐binding lysozymes. Thus, from the point of view of the unfolding profile, equine lysozyme belongs to the group of α‐lactalbumin, but pigeon lysozyme belongs to the conventional lysozyme group.

Bibliography

NITTA, K., TSUGE, H., & IWAMOTO, H. (1993). Comparative study of the stability of the folding intermediates of the calciumâbinding lysozymes. International Journal of Peptide and Protein Research, 41(2), 118â123. Portico.

Authors 3

KATSUTOSHI NITTA (first)
HIDEAKI TSUGE (additional)
HIROSHI IWAMOTO (additional)

References 16 Referenced 21

10.1016/0006-291X(80)91585-5
10.1111/j.1432-1033.1989.tb14806.x
10.1016/S0065-3233(08)60198-9
10.1016/0022-2836(76)90091-7
10.1016/0014-5793(87)80328-9
10.1515/bchm3.1988.369.2.671
{'key': 'e_1_2_2_8_2', 'first-page': '185', 'volume': '37', 'author': 'Desmet J.', 'year': '1989', 'journal-title': 'J. Inorg. Chem.'} / J. Inorg. Chem. by Desmet J. (1989)
{'key': 'e_1_2_2_9_2', 'first-page': '101', 'volume-title': 'Buffers for pH and Metal Ion Control', 'author': 'Perrin D.D.', 'year': '1974'} / Buffers for pH and Metal Ion Control by Perrin D.D. (1974)
10.1021/bi00370a034
10.1021/bi00427a002
10.1016/0305-0491(87)90245-8
10.1002/bip.1973.360121107
10.1016/0022-2836(77)90208-X
10.1016/0005-2795(77)90120-9
10.1016/S0065-3233(08)60241-7
10.1021/bi00224a018

Dates

Type	When
Created	15 years ago (Aug. 9, 2010, 5:48 p.m.)
Deposited	1 year, 9 months ago (Oct. 25, 2023, 8:44 p.m.)
Indexed	1 month, 2 weeks ago (July 4, 2025, 7:48 a.m.)
Issued	32 years, 6 months ago (Feb. 1, 1993)
Published	32 years, 6 months ago (Feb. 1, 1993)
Published Online	16 years, 7 months ago (Jan. 12, 2009)
Published Print	32 years, 6 months ago (Feb. 1, 1993)

Funders 0

None

BibTeX

@article{NITTA_1993, title={Comparative study of the stability of the folding intermediates of the calcium‐binding lysozymes}, volume={41}, ISSN={0367-8377}, url={http://dx.doi.org/10.1111/j.1399-3011.1993.tb00121.x}, DOI={10.1111/j.1399-3011.1993.tb00121.x}, number={2}, journal={International Journal of Peptide and Protein Research}, publisher={Wiley}, author={NITTA, KATSUTOSHI and TSUGE, HIDEAKI and IWAMOTO, HIROSHI}, year={1993}, month=feb, pages={118–123} }

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