DOI: 10.1111/j.1399-3011.1985.tb03179.x. Single chain des‐(B30) insulin

10.1111/j.1399-3011.1985.tb03179.x

Crossref journal-article

Wiley

International Journal of Peptide and Protein Research (311)

Abstract

Single chain des‐(B30) insulin (SCI) has been synthesized from porcine insulin by trypsin in a medium with a low content of water. Trypsin catalyzes an intramolecular transpeptidation reaction in which the glycineA1 residue substitutes the alanineB30 residue, rendering a LysB29‐GlyA1 peptide link between the A‐and B‐chains of insulin. The insulin derivative has been purified by column chromatography and appears to be homogeneous in HPLC and disc electrophoresis. The structure was proven to be B(1–29)–A(1–21) insulin by proteolysis with Armilliaria mellea protease followed by a few steps of Edman degradation. The electrophoretic mobility indicates that SCI has a more condensed structure than that of insulin. Perfect rhombohedral crystals were obtained under conditions resembling those under which insulin crystallizes in the same form. SCI was devoid of effect in the blood sugar lowering assay in mice, the estimated potency being less than 0.1% of that of insulin.

Bibliography

MARKUSSEN, J., JÃRGENSEN, K. H., SÃRENSEN, A. R., & THIM, L. (1985). Single chain desâ(B30) insulin. International Journal of Peptide and Protein Research, 26(1), 70â77. Portico.

Authors 4

JAN MARKUSSEN (first)
KLAVS H. JØRGENSEN (additional)
ANDERS R. SØRENSEN (additional)
LARS THIM (additional)

References 21 Referenced 64

{'key': 'e_1_2_1_2_2', 'first-page': '50', 'volume-title': 'Proceedings of the Symposium on Pro‐insulin, Insulin and C‐Peptide', 'author': 'Markussen J.', 'year': '1978'} / Proceedings of the Symposium on Pro‐insulin, Insulin and C‐Peptide by Markussen J. (1978)
Markussen J.(1982)US patent 4 343.8984.
{'key': 'e_1_2_1_4_2', 'first-page': '387', 'volume-title': 'Proceedings of the 17th European Peptide Symposium', 'author': 'Markussen J.', 'year': '1983'} / Proceedings of the 17th European Peptide Symposium by Markussen J. (1983)
10.1021/bi00602a015
{'key': 'e_1_2_1_6_2', 'first-page': '834', 'volume-title': 'National Formulary NF XV, U.S. Pharmacopaeia USP XX', 'year': '1980'} / National Formulary NF XV, U.S. Pharmacopaeia USP XX (1980)
10.1016/0005-2744(78)90087-6
10.1016/0014-5793(78)81105-3
10.3891/acta.chem.scand.10-1459
10.3891/acta.chem.scand.10-1455
10.1093/clinchem/26.1.123 / Clin. Chem. by Leon L.P. (1980)
10.1155/S1463924683000462
10.1111/j.1749-6632.1964.tb14213.x
{'key': 'e_1_2_1_14_2', 'first-page': '111', 'volume-title': 'Handbuch der Inneren Medizin', 'author': 'Schlichtkrull J.', 'year': '1975'} / Handbuch der Inneren Medizin by Schlichtkrull J. (1975)
10.1021/bi00901a005
10.1515/bchm2.1972.353.1.599
10.1515/bchm2.1971.352.1.719
{'key': 'e_1_2_1_18_2', 'first-page': '270', 'volume-title': 'Proceedings of the 12th European Peptide Symposium', 'author': 'Brandenburg D.', 'year': '1973'} / Proceedings of the 12th European Peptide Symposium by Brandenburg D. (1973)
10.1038/259369a0
10.1038/231506a0
10.1515/bchm2.1981.362.1.647
{'key': 'e_1_2_1_22_2', 'first-page': '207', 'volume-title': 'Proceedings of the 8th American Peptide Symposium', 'author': 'Markussen J.', 'year': '1983'} / Proceedings of the 8th American Peptide Symposium by Markussen J. (1983)

Dates

Type	When
Created	15 years ago (Aug. 9, 2010, 2:11 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 5:25 p.m.)
Indexed	1 year, 5 months ago (April 3, 2024, 2:30 p.m.)
Issued	40 years, 2 months ago (July 1, 1985)
Published	40 years, 2 months ago (July 1, 1985)
Published Online	16 years, 7 months ago (Jan. 12, 2009)
Published Print	40 years, 2 months ago (July 1, 1985)

Funders 0

None

BibTeX

@article{MARKUSSEN_1985, title={Single chain des‐(B30) insulin: Intramolecular crosslinking of insulin by trypsin catalyzed transpeptidation}, volume={26}, ISSN={0367-8377}, url={http://dx.doi.org/10.1111/j.1399-3011.1985.tb03179.x}, DOI={10.1111/j.1399-3011.1985.tb03179.x}, number={1}, journal={International Journal of Peptide and Protein Research}, publisher={Wiley}, author={MARKUSSEN, JAN and JØRGENSEN, KLAVS H. and SØRENSEN, ANDERS R. and THIM, LARS}, year={1985}, month=jul, pages={70–77} }

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