DOI: 10.1111/j.1399-3011.1969.tb01636.x. A STUDY OF THE THERMAL DENATURATION OF RIBONUCLEASE BY DIFFERENTIAL SCANNING CALORIMETRY

A STUDY OF THE THERMAL DENATURATION OF RIBONUCLEASE BY DIFFERENTIAL SCANNING CALORIMETRY

10.1111/j.1399-3011.1969.tb01636.x

Crossref journal-article

Wiley

International Journal of Protein Research (311)

Abstract

A study by differential scanning calorimetry (DSC) of ribonuclease thermal denaturation in water, 1.5, 2.5, and 4M aqueous urea, and in 1M and 2M aqueous hexame‐thylenetetramine has been carried out. Application of the DSC technique to this type of investigation appears a useful one, in our experience. Our results indicate that whereas urea only slightly changes enthalpy variation accompanying denaturation but lowers the melting temperature of ribonuclease, hexamethylenetetramine does not effect either factor. According to these results, which are beyond experimental errors, the ability of hexamethylenetetramine in influencing hydrophobic‐forces‐driven phenomena (Barone, Crescenzi & Quadrifoglio 1967) has thus apparently no counterpart in any important action of this compound on the stability of a partially hydrophobic protein in aqueous solution.

Bibliography

Delben, F., Crescenzi, V., & Quadrifoglio, F. (1969). A STUDY OF THE THERMAL DENATURATION OF RIBONUCLEASE BY DIFFERENTIAL SCANNING CALORIMETRY. International Journal of Protein Research, 1(1â4), 145â149. Portico.

Authors 3

F. Delben (first)
V. Crescenzi (additional)
F. Quadrifoglio (additional)

References 12 Referenced 22

10.1021/j100863a034
10.1021/ja01082a036
10.1021/ja00995a002
10.1021/j100847a027
10.1021/j100866a054
10.1063/1.1720742
10.1021/ja01632a065
10.1021/ja00972a009
10.1016/0005-2736(68)90132-6
10.1021/ja00906a028
{'key': 'e_1_2_1_12_1', 'volume-title': 'Formaldehyde', 'author': 'Walker J. F.', 'year': '1944'} / Formaldehyde by Walker J. F. (1944)
10.1021/ac60213a019

Dates

Type	When
Created	15 years ago (Aug. 18, 2010, 4:20 a.m.)
Deposited	2 years ago (Aug. 30, 2023, 6:35 a.m.)
Indexed	1 year, 1 month ago (July 9, 2024, 6:28 p.m.)
Issued	55 years, 8 months ago (Dec. 1, 1969)
Published	55 years, 8 months ago (Dec. 1, 1969)
Published Online	16 years, 7 months ago (Jan. 9, 2009)
Published Print	55 years, 8 months ago (Dec. 1, 1969)

Funders 0

None

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