DOI: 10.1111/j.1399-0039.1995.tb02482.x. A comprehensive approach for typing the alleles of the HLA‐B locus by automated sequencing

A comprehensive approach for typing the alleles of the HLA‐B locus by automated sequencing

10.1111/j.1399-0039.1995.tb02482.x

Crossref journal-article

Wiley

Tissue Antigens (311)

Abstract

Abstract: We describe an approach for typing alleles of the HLA‐B locus by using automated sequencing technology. The exon 2 and exon 3 nucleotide sequence of each allele is determined directly from genomic DNA in two steps. In the first step, HLA‐B exon 2, intron 2 and exon 3 sequences are amplified with one or two primer pairs out of a panel of 5 primer pairs that describe all known HLA‐B alleles. In the second step, templates are sequenced in 5′ and 3′ orientations in a PCR assay that utilizes Taq polymerase to incorporate fluorescent dye‐labeled nucleotides into each new strand synthesized. Gel electrophoresis of the labeled products is performed in an automated DNA sequencer. The derived sequences are aligned against reference sequences and each nucleotide position is evaluated for homology to consensus sequence. Using this strategy, the HLA‐B allele sequence is directly ascertained with precision and efficiency. The automated sequencing strategy can be readily applied in the clinical laboratory as a practical tool for high resolution typing of HLA‐B alleles.

Bibliography

Petersdorf, E. W., & Hansen, J. A. (1995). A comprehensive approach for typing the alleles of the HLAâB locus by automated sequencing. Tissue Antigens, 46(2), 73â85. Portico.

Authors 2

E. W. Petersdorf (first)
J. A. Hansen (additional)

References 34 Referenced 79

10.1016/0198-8859(92)90068-X
10.1073/pnas.90.7.2842
10.1111/j.1399-0039.1993.tb01992.x
10.1111/j.1399-0039.1994.tb02371.x
10.1016/0198-8859(92)90025-I
10.1111/j.1399-0039.1994.tb02372.x
10.1111/j.1399-0039.1994.tb02290.x
10.1111/j.1399-0039.1994.tb02376.x
10.1111/j.1399-0039.1992.tb01940.x
10.1016/0198-8859(91)90014-Z
10.1007/BF00187094
10.1016/0198-8859(93)90555-F
10.1073/pnas.85.11.4005
10.4049/jimmunol.148.1.249 / J Immunol / Polymorphism, recombination, and linkage disequilibrium within the HLA class II region by Begovich AB (1992)
{'key': 'e_1_2_1_16_2', 'first-page': '1065', 'volume-title': 'HLA 1991', 'author': 'Imanishi T', 'year': '1992'} / HLA 1991 by Imanishi T (1992)
10.1073/pnas.85.16.6012
10.1056/NEJM199012273232607
10.1182/blood.V81.7.1923.1923
10.1016/0198-8859(93)90548-F
{'key': 'e_1_2_1_21_2', 'first-page': '137', 'article-title': 'HLA‐B44‐di‐rected cytotoxic T cells associated with acute graft‐versus‐host disease following unrelated bone marrow transplantation', 'volume': '14', 'author': 'Keever CA', 'year': '1994', 'journal-title': 'Bone Marrow Transplant'} / Bone Marrow Transplant / HLA‐B44‐di‐rected cytotoxic T cells associated with acute graft‐versus‐host disease following unrelated bone marrow transplantation by Keever CA (1994)
10.1111/j.1399-0039.1994.tb02351.x
10.1111/j.1399-0039.1994.tb02364.x
10.1111/j.1399-0039.1994.tb02385.x
10.1016/0198-8859(93)90141-M
{'key': 'e_1_2_1_26_2', 'article-title': 'Identification of two major HLA‐B44 subtypes and a novel B44 sequence: oligotyping and solid phase sequencing of PCR products', 'author': 'Yao Z', 'journal-title': 'Hum Immunol'} / Hum Immunol / Identification of two major HLA‐B44 subtypes and a novel B44 sequence: oligotyping and solid phase sequencing of PCR products by Yao Z
10.1111/j.1399-0039.1994.tb02386.x
10.1016/0198-8859(93)90503-S
10.1111/j.1744-313X.1993.tb00111.x
10.1006/geno.1994.1469
{'key': 'e_1_2_1_31_2', 'first-page': '618', 'article-title': 'Screening for point mutations by semi‐automated DNA sequencing using Sequenaser̀ and magnetic beads', 'volume': '14', 'author': 'Leren TP', 'year': '1993', 'journal-title': 'Bio Techniques'} / Bio Techniques / Screening for point mutations by semi‐automated DNA sequencing using Sequenaser̀ and magnetic beads by Leren TP (1993)
10.1111/j.1399-0039.1994.tb02327.x
10.1016/0198-8859(93)90514-2
10.1111/j.1399-0039.1994.tb02402.x
10.1111/j.1399-0039.1995.tb02469.x

Dates

Type	When
Created	16 years, 8 months ago (Dec. 11, 2008, 11:05 a.m.)
Deposited	1 year, 10 months ago (Oct. 26, 2023, 7:22 p.m.)
Indexed	1 year, 8 months ago (Dec. 24, 2023, 6:29 p.m.)
Issued	30 years, 1 month ago (Aug. 1, 1995)
Published	30 years, 1 month ago (Aug. 1, 1995)
Published Online	16 years, 8 months ago (Dec. 11, 2008)
Published Print	30 years, 1 month ago (Aug. 1, 1995)

Funders 0

None

BibTeX

@article{Petersdorf_1995, title={A comprehensive approach for typing the alleles of the HLA‐B locus by automated sequencing}, volume={46}, ISSN={1399-0039}, url={http://dx.doi.org/10.1111/j.1399-0039.1995.tb02482.x}, DOI={10.1111/j.1399-0039.1995.tb02482.x}, number={2}, journal={Tissue Antigens}, publisher={Wiley}, author={Petersdorf, E. W. and Hansen, J. A.}, year={1995}, month=aug, pages={73–85} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        12,
        24
      ]
    ],
    "date-time": "2023-12-24T23:29:46Z",
    "timestamp": 1703460586219
  },
  "reference-count": 34,
  "publisher": "Wiley",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2008,
            12,
            11
          ]
        ],
        "date-time": "2008-12-11T00:00:00Z",
        "timestamp": 1228953600000
      },
      "content-version": "vor",
      "delay-in-days": 4881,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        8
      ]
    ]
  },
  "abstract": "<jats:p><jats:bold>Abstract: </jats:bold> We describe an approach for typing alleles of the HLA\u2010B locus by using automated sequencing technology. The exon 2 and exon 3 nucleotide sequence of each allele is determined directly from genomic DNA in two steps. In the first step, HLA\u2010B exon 2, intron 2 and exon 3 sequences are amplified with one or two primer pairs out of a panel of 5 primer pairs that describe all known HLA\u2010B alleles. In the second step, templates are sequenced in 5\u2032 and 3\u2032 orientations in a PCR assay that utilizes Taq polymerase to incorporate fluorescent dye\u2010labeled nucleotides into each new strand synthesized. Gel electrophoresis of the labeled products is performed in an automated DNA sequencer. The derived sequences are aligned against reference sequences and each nucleotide position is evaluated for homology to consensus sequence. Using this strategy, the HLA\u2010B allele sequence is directly ascertained with precision and efficiency. The automated sequencing strategy can be readily applied in the clinical laboratory as a practical tool for high resolution typing of HLA\u2010B alleles.</jats:p>",
  "DOI": "10.1111/j.1399-0039.1995.tb02482.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2008,
        12,
        11
      ]
    ],
    "date-time": "2008-12-11T16:05:52Z",
    "timestamp": 1229011552000
  },
  "page": "73-85",
  "source": "Crossref",
  "is-referenced-by-count": 79,
  "title": "A comprehensive approach for typing the alleles of the HLA\u2010B locus by automated sequencing",
  "prefix": "10.1111",
  "volume": "46",
  "author": [
    {
      "given": "E. W.",
      "family": "Petersdorf",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "J. A.",
      "family": "Hansen",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2008,
        12,
        11
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0198-8859(92)90068-X"
    },
    {
      "key": "e_1_2_1_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.90.7.2842"
    },
    {
      "key": "e_1_2_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-0039.1993.tb01992.x"
    },
    {
      "key": "e_1_2_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-0039.1994.tb02371.x"
    },
    {
      "key": "e_1_2_1_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0198-8859(92)90025-I"
    },
    {
      "key": "e_1_2_1_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-0039.1994.tb02372.x"
    },
    {
      "key": "e_1_2_1_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-0039.1994.tb02290.x"
    },
    {
      "key": "e_1_2_1_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-0039.1994.tb02376.x"
    },
    {
      "key": "e_1_2_1_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-0039.1992.tb01940.x"
    },
    {
      "key": "e_1_2_1_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0198-8859(91)90014-Z"
    },
    {
      "key": "e_1_2_1_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00187094"
    },
    {
      "key": "e_1_2_1_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0198-8859(93)90555-F"
    },
    {
      "key": "e_1_2_1_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.85.11.4005"
    },
    {
      "key": "e_1_2_1_15_2",
      "doi-asserted-by": "crossref",
      "first-page": "249",
      "DOI": "10.4049/jimmunol.148.1.249",
      "article-title": "Polymorphism, recombination, and linkage disequilibrium within the HLA class II region",
      "volume": "148",
      "author": "Begovich AB",
      "year": "1992",
      "journal-title": "J Immunol"
    },
    {
      "key": "e_1_2_1_16_2",
      "first-page": "1065",
      "volume-title": "HLA 1991",
      "author": "Imanishi T",
      "year": "1992"
    },
    {
      "key": "e_1_2_1_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.85.16.6012"
    },
    {
      "key": "e_1_2_1_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1056/NEJM199012273232607"
    },
    {
      "key": "e_1_2_1_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1182/blood.V81.7.1923.1923"
    },
    {
      "key": "e_1_2_1_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0198-8859(93)90548-F"
    },
    {
      "key": "e_1_2_1_21_2",
      "first-page": "137",
      "article-title": "HLA\u2010B44\u2010di\u2010rected cytotoxic T cells associated with acute graft\u2010versus\u2010host disease following unrelated bone marrow transplantation",
      "volume": "14",
      "author": "Keever CA",
      "year": "1994",
      "journal-title": "Bone Marrow Transplant"
    },
    {
      "key": "e_1_2_1_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-0039.1994.tb02351.x"
    },
    {
      "key": "e_1_2_1_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-0039.1994.tb02364.x"
    },
    {
      "key": "e_1_2_1_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-0039.1994.tb02385.x"
    },
    {
      "key": "e_1_2_1_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0198-8859(93)90141-M"
    },
    {
      "key": "e_1_2_1_26_2",
      "article-title": "Identification of two major HLA\u2010B44 subtypes and a novel B44 sequence: oligotyping and solid phase sequencing of PCR products",
      "author": "Yao Z",
      "journal-title": "Hum Immunol"
    },
    {
      "key": "e_1_2_1_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-0039.1994.tb02386.x"
    },
    {
      "key": "e_1_2_1_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0198-8859(93)90503-S"
    },
    {
      "key": "e_1_2_1_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1744-313X.1993.tb00111.x"
    },
    {
      "key": "e_1_2_1_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/geno.1994.1469"
    },
    {
      "key": "e_1_2_1_31_2",
      "first-page": "618",
      "article-title": "Screening for point mutations by semi\u2010automated DNA sequencing using Sequenaser\u0300 and magnetic beads",
      "volume": "14",
      "author": "Leren TP",
      "year": "1993",
      "journal-title": "Bio Techniques"
    },
    {
      "key": "e_1_2_1_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-0039.1994.tb02327.x"
    },
    {
      "key": "e_1_2_1_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0198-8859(93)90514-2"
    },
    {
      "key": "e_1_2_1_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-0039.1994.tb02402.x"
    },
    {
      "key": "e_1_2_1_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1399-0039.1995.tb02469.x"
    }
  ],
  "container-title": "Tissue Antigens",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1399-0039.1995.tb02482.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1399-0039.1995.tb02482.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        26
      ]
    ],
    "date-time": "2023-10-26T23:22:07Z",
    "timestamp": 1698362527000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1399-0039.1995.tb02482.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        8
      ]
    ]
  },
  "references-count": 34,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1995,
          8
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1399-0039.1995.tb02482.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1399-0039.1995.tb02482.x",
  "relation": {},
  "ISSN": [
    "0001-2815",
    "1399-0039"
  ],
  "subject": [],
  "container-title-short": "Tissue Antigens",
  "published": {
    "date-parts": [
      [
        1995,
        8
      ]
    ]
  }
}