DOI: 10.1111/j.1365-313x.2007.03108.x. Members of the <i>PHO1</i> gene family show limited functional redundancy in phosphate transfer to the shoot, and are regulated by phosphate deficiency via distinct pathways

Members of the PHO1 gene family show limited functional redundancy in phosphate transfer to the shoot, and are regulated by phosphate deficiency via distinct pathways

10.1111/j.1365-313x.2007.03108.x

Crossref journal-article

Wiley

The Plant Journal (311)

Abstract

SummaryThe PHO1 family comprises 11 members in Arabidopsis thaliana. In order to decipher the role of these genes in inorganic phosphate (Pi) transport and homeostasis, complementation of the pho1 mutant, deficient in loading Pi to the root xylem, was determined by the expression of the PHO1 homologous genes under the control of the PHO1 promoter. Only PHO1 and the homologue PHO1;H1 could complement pho1. The PHO1;H1 promoter was active in the vascular cylinder of roots and shoots. Expression of PHO1;H1 was very low in Pi‐sufficient plants, but was strongly induced under Pi‐deficient conditions. T‐DNA knock‐out mutants of PHO1;H1 neither showed growth defects nor alteration in Pi transport dynamics, or Pi content, compared with wild type. However, the double mutant pho1/pho1;h1 showed a strong reduction in growth and in the capacity to transfer Pi from the root to the shoot compared with pho1. Grafting experiments revealed that phenotypes associated with the pho1 and pho1/pho1;h1 mutants were linked to the lack of gene expression in the root. The increased expression of PHO1;H1 under Pi deficiency was largely controlled by the transcription factor PHR1 and was suppressed by the phosphate analogue phosphite, whereas the increase of PHO1 expression was independent of PHR1 and was not influenced by phosphite. Together, these data reveal that although transfer of Pi to the root xylem vessel is primarily mediated by PHO1, the homologue PHO1;H1 also contributes to Pi loading to the xylem, and that the two corresponding genes are regulated by Pi deficiency by distinct signal transduction pathways.

Bibliography

Stefanovic, A., Ribot, C., Rouached, H., Wang, Y., Chong, J., Belbahri, L., Delessert, S., & Poirier, Y. (2007). Members of the PHO1 gene family show limited functional redundancy in phosphate transfer to the shoot, and are regulated by phosphate deficiency via distinct pathways. The Plant Journal, 50(6), 982â994. Portico.

Authors 8

Aleksandra Stefanovic (first)
Cécile Ribot (additional)
Hatem Rouached (additional)
Yong Wang (additional)
Julie Chong (additional)
Lassaad Belbahri (additional)
Syndie Delessert (additional)
Yves Poirier (additional)

References 44 Referenced 183

10.1126/science.1086391
10.1016/0076-6879(66)08014-5
10.1104/pp.106.078063
10.1104/pp.106.079707
10.1111/j.1365-3040.1996.tb00386.x
10.1104/pp.010397
10.1016/S0168-9452(00)00460-X
10.1104/pp.110.1.105
10.1007/s00050166
10.1007/s004250000271
10.1105/tpc.105.038943
10.1016/0003-2697(87)90021-2
10.1046/j.1365-313x.1998.00343.x / Plant J. / Floral dip: a simplified method for Agrobacterium‐mediated transformation of Arabidopsis thaliana by Clough S.J. (1998)
10.1104/pp.103.027979
10.1104/pp.107.1.207
10.1146/annurev.arplant.50.1.27
10.1093/jxb/erh009
10.1046/j.1365-313x.2001.01016.x
10.1105/tpc.000745
10.1105/tpc.105.037879
10.1111/j.1365-313X.2006.02778.x
10.1105/tpc.11.12.2283
10.1046/j.1365-313X.1996.09020195.x
10.1046/j.1365-313X.1999.00480.x
10.1046/j.1365-313X.2002.01251.x
10.1046/j.1365-313x.2000.00893.x
10.1016/S0074-7696(08)60159-X
10.1073/pnas.0500778102
10.1046/j.1365-313X.2002.01356.x
10.1093/nar/29.9.e45
10.1199/tab.0024
10.1104/pp.97.3.1087
10.1634/stemcells.21-4-459
10.1007/s00425-002-0921-3
10.1105/tpc.12.5.707 / Plant Cell / Differential gene expression in response to mechanical wounding and insect feeding in Arabidopsis by Reymond P. (2000)
10.1101/gad.204401
{'key': 'e_1_2_6_38_1', 'volume-title': 'Molecular cloning, a laboratory manual', 'author': 'Sambrook J.', 'year': '1989'} / Molecular cloning, a laboratory manual by Sambrook J. (1989)
10.1111/j.1365-313X.2005.02629.x
10.1104/pp.010396
10.1111/j.1365-313X.2004.02005.x
10.1046/j.1365-313X.2002.01419.x
10.1104/pp.010835
10.1105/tpc.002220
10.1104/pp.103.037945

Dates

Type	When
Created	18 years, 3 months ago (May 6, 2007, 12:53 p.m.)
Deposited	1 year, 10 months ago (Oct. 9, 2023, 9:42 p.m.)
Indexed	39 minutes ago (Aug. 26, 2025, 7:21 p.m.)
Issued	18 years, 3 months ago (May 23, 2007)
Published	18 years, 3 months ago (May 23, 2007)
Published Online	18 years, 3 months ago (May 23, 2007)
Published Print	18 years, 2 months ago (June 1, 2007)

Funders 0

None

BibTeX

@article{Stefanovic_2007, title={Members of the PHO1 gene family show limited functional redundancy in phosphate transfer to the shoot, and are regulated by phosphate deficiency via distinct pathways}, volume={50}, ISSN={1365-313X}, url={http://dx.doi.org/10.1111/j.1365-313x.2007.03108.x}, DOI={10.1111/j.1365-313x.2007.03108.x}, number={6}, journal={The Plant Journal}, publisher={Wiley}, author={Stefanovic, Aleksandra and Ribot, Cécile and Rouached, Hatem and Wang, Yong and Chong, Julie and Belbahri, Lassaad and Delessert, Syndie and Poirier, Yves}, year={2007}, month=may, pages={982–994} }

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