The Etched1 gene of Zea mays (L.) encodes a zinc ribbon protein that belongs to the transcriptionally active chromosome (TAC) of plastids and is similar to the transcription factor TFIIS
10.1111/j.1365-313x.2004.02094.x
Crossref journal-article
Wiley
The Plant Journal (311)
Abstract

Summary Etched1 (et1) is a pleiotropic, recessive mutation of maize that causes fissured and cracked mature kernels and virescent seedlings. Microscopic examinations of the et1 phenotype revealed an aberrant plastid development in mutant kernels and mutant leaves. Here, we report on the cloning of the et1 gene by transposon tagging, the localization of the gene product in chloroplasts, and its putative function in the plastid transcriptional apparatus. Several alleles of Mutator (Mu)‐induced et1 mutants, the et1‐reference (et1‐R) mutant, and Et1 wild‐type were cloned and analyzed at the molecular level. Northern analyses with wild‐type plants revealed that Et1 transcripts are present in kernels, leaves, and other types of tissue, and no Et1 expression could be detected in the et1 mutants analyzed. The ET1 protein is imported by chloroplasts and has been immunologically detected in transcriptionally active chromosome (TAC) fractions derived from chloroplasts. Accordingly, the relative transcriptional activity of TAC fractions was significantly reduced in chloroplasts of et1‐R plants. ET1 is the first zinc ribbon (ZR) protein shown to be targeted to plastids. With regard to its localization and its striking structural similarity to the eukaryotic transcription elongation factor TFIIS, it is feasible that ET1 functions in plastid transcription elongation by reactivation of arrested RNA polymerases.

Bibliography

Da Costa e Silva, O., Lorbiecke, R., Garg, P., Müller, L., Waßmann, M., Lauert, P., Scanlon, M., Hsia, A., Schnable, P. S., Krupinska, K., & Wienand, U. (2004). The Etched1 gene of Zea mays (L.) encodes a zinc ribbon protein that belongs to the transcriptionally active chromosome (TAC) of plastids and is similar to the transcription factor TFIIS. The Plant Journal, 38(6), 923–939. Portico.

Authors 11
  1. Oswaldo Da Costa e Silva (first)
  2. René Lorbiecke (additional)
  3. Preeti Garg (additional)
  4. Lenard Müller (additional)
  5. Martina Waßmann (additional)
  6. Patricia Lauert (additional)
  7. Mike Scanlon (additional)
  8. An‐Ping Hsia (additional)
  9. Patrick S. Schnable (additional)
  10. Karin Krupinska (additional)
  11. Udo Wienand (additional)
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Dates
Type When
Created 21 years, 3 months ago (May 24, 2004, 10:47 a.m.)
Deposited 1 year, 11 months ago (Sept. 29, 2023, 12:05 a.m.)
Indexed 1 month, 1 week ago (July 28, 2025, 5:28 p.m.)
Issued 21 years, 4 months ago (April 29, 2004)
Published 21 years, 4 months ago (April 29, 2004)
Published Online 21 years, 4 months ago (April 29, 2004)
Published Print 21 years, 3 months ago (June 1, 2004)
Funders 0

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@article{Da_Costa_e_Silva_2004, title={The Etched1 gene of Zea mays (L.) encodes a zinc ribbon protein that belongs to the transcriptionally active chromosome (TAC) of plastids and is similar to the transcription factor TFIIS}, volume={38}, ISSN={1365-313X}, url={http://dx.doi.org/10.1111/j.1365-313x.2004.02094.x}, DOI={10.1111/j.1365-313x.2004.02094.x}, number={6}, journal={The Plant Journal}, publisher={Wiley}, author={Da Costa e Silva, Oswaldo and Lorbiecke, René and Garg, Preeti and Müller, Lenard and Waßmann, Martina and Lauert, Patricia and Scanlon, Mike and Hsia, An‐Ping and Schnable, Patrick S. and Krupinska, Karin and Wienand, Udo}, year={2004}, month=apr, pages={923–939} }