DOI: 10.1111/j.1365-3083.1989.tb02476.x. Impaired Generation of Anti‐Viral Cytotoxicity against Lymphocytic Choriomeningitis and Vaccinia Virus in Mice Treated with CD4‐Specific Monoclonal Antibody

Impaired Generation of Anti‐Viral Cytotoxicity against Lymphocytic Choriomeningitis and Vaccinia Virus in Mice Treated with CD4‐Specific Monoclonal Antibody

10.1111/j.1365-3083.1989.tb02476.x

Crossref journal-article

Wiley

Scandinavian Journal of Immunology (311)

Abstract

The role of CD4+ helper T cells in induction of anti‐viral cytotoxic T‐cell response was investigated by treating normal and thymectomized C57B1/6 mice with CD4‐speciflc monoclonal antibodies (MoAb). In CD4‐specific MoAb‐treated mice infected with Vaccinia or lymphocytic choriomeningitis virus (LCMV), cytotoxic T‐cell activity was 5–15 times lower than in normal controls when measured in a 51Cr release assay and computed as lytic units 6 and 8 days respectively after virus inoculation. This difference in the levels of effector T‐cell activities did not reflect slower kinetics of cytotoxic T‐cell induction in antibody‐treated versus control mice, since it was also obvious at 8 days after infection for Vaccinia virus and 10 and 12 days after inoculation with LCMV, CD4‐specific MoAb‐induced inhibition of cytotoxic T‐cell responses in vivo was seen up to 150 days after treatment in thymectomized mice. However, no significant suppressive effect of the same antibody treatment on T‐cell cytotoxicity could be observed in animals treated on day 3 or later after infection with Vaccinia virus. Injection of CD4‐depleted mice with recombinant interleukin 2 (rIL‐2) partially corrected the impaired virus‐specific cytotoxic T‐cell response, suggesting that IL‐2 supply may be limiting in mice licking T helper cells.

Bibliography

LEIST, T. P., KOHLER, M., & ZINKERNAGEL, R. M. (1989). Impaired Generation of AntiâViral Cytotoxicity against Lymphocytic Choriomeningitis and Vaccinia Virus in Mice Treated with CD4âSpecific Monoclonal Antibody. Scandinavian Journal of Immunology, 30(6), 679â686. Portico.

Authors 3

T. P. LEIST (first)
M. KOHLER (additional)
R. M. ZINKERNAGEL (additional)

References 27 Referenced 39

10.1128/jvi.62.6.2102-2106.1988 / J. Virol. / T4+ T helper cell function in vivo: differential requirement for induction of antiviral cytotoxic T‐cell and antibody responses by Ahmed R. (1988)
10.1084/jem.150.5.1277
10.1111/j.1600-065X.1977.tb00236.x
10.1038/276829a0
10.1016/S0065-2776(08)60197-2
10.1038/328077a0
10.1084/jem.141.6.1376
10.1016/S0065-2776(08)60308-9
10.4049/jimmunol.115.3.844 / J. Immunol. / Activated macrophages in congenically athymic ‘nude’ mice and in lethally irradiated mice by Cheers C. (1975)
10.1038/312548a0
10.1111/j.1600-065X.1983.tb01083.x
10.4049/jimmunol.123.3.1205 / J. Immunol. / The cytolytic T lymphocyte response to trinitrophenyl‐modified syngeneic cells by Finberg R. (1979)
10.4049/jimmunol.135.1.39 / J. Immunol / A role for helper cell subpopulations in genetically controlled cytolytic T lymphocyte response to the H‐2Db antigen by French D.L. (1985)
10.4049/jimmunol.127.6.2291 / J. Immunol. / Accessory cells in the in vitro generation of type c virus‐specific T killer lymphocytes by Gomard E. (1981)
10.1084/jem.164.3.723
10.4049/jimmunol.138.7.2278 / J. Immunol. / Functional analysis of T lymphocyte subsets in antiviral host defense by Leist T.P. (1987)
10.1111/j.1749-6632.1966.tb12851.x
10.1016/0008-8749(76)90359-2
10.1084/jem.154.2.541
10.1093/jnci/54.6.1419 / J. Natl. Cancer. Inst / Response of congenitally athymic (nude) mice to infection with Mycobacterium bovis (strain BCG) by Sher N.A. (1975)
10.1111/j.1600-065X.1977.tb00235.x
10.1084/jem.150.5.1134
10.1111/j.1600-065X.1984.tb01117.x
10.1007/BF01932477
10.1084/jem.147.3.897
10.1084/jem.162.6.2125
10.1084/jem.168.3.1187

Dates

Type	When
Created	19 years, 1 month ago (June 30, 2006, 5:40 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 2:18 a.m.)
Indexed	4 months, 3 weeks ago (March 31, 2025, 6:04 a.m.)
Issued	35 years, 8 months ago (Dec. 1, 1989)
Published	35 years, 8 months ago (Dec. 1, 1989)
Published Online	19 years, 1 month ago (June 29, 2006)
Published Print	35 years, 8 months ago (Dec. 1, 1989)

Funders 0

None

BibTeX

@article{LEIST_1989, title={Impaired Generation of Anti‐Viral Cytotoxicity against Lymphocytic Choriomeningitis and Vaccinia Virus in Mice Treated with CD4‐Specific Monoclonal Antibody}, volume={30}, ISSN={1365-3083}, url={http://dx.doi.org/10.1111/j.1365-3083.1989.tb02476.x}, DOI={10.1111/j.1365-3083.1989.tb02476.x}, number={6}, journal={Scandinavian Journal of Immunology}, publisher={Wiley}, author={LEIST, T. P. and KOHLER, M. and ZINKERNAGEL, R. M.}, year={1989}, month=dec, pages={679–686} }

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