DOI: 10.1111/j.1365-2958.2011.07964.x. Interaction of a bacterial flagellar chaperone FlgN with FlhA is required for efficient export of its cognate substrates

Interaction of a bacterial flagellar chaperone FlgN with FlhA is required for efficient export of its cognate substrates

10.1111/j.1365-2958.2011.07964.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryFlgN chaperone acts as a bodyguard to protect its cognate substrates, FlgK and FlgL, from proteolysis in the cytoplasm. Docking of the FlgN–FlgK complex with the FliI ATPase of the flagellar type III export apparatus is key to the protein export process. However, a ΔfliH‐fliI flhB(P28T) mutant forms some flagella even in the absence of FliH and FliI, raising the question of how FlgN promotes the export of its cognate substrates. Here, we report that the interaction of FlgN with an integral membrane export protein, FlhA, is directly involved in efficient protein export. A ΔfliH‐fliI flhB(P28T) ΔflgN mutant caused extragenic suppressor mutations in the C‐terminal domain of FlhA (FlhAC). Pull‐down assays using GST affinity chromatography showed an interaction between FlgN and FlhAC. The FlgN–FlgK complex bound to FlhAC and FliJ to form the FlgN–FlgK–FliJ–FlhAC complex. The FlgN–FlhAC interaction was enhanced by FlgK but not by FliJ. FlgN120 missing the last 20 residues still bound to FlgK and FliJ but not to FlhAC. A highly conserved Tyr‐122 residue was required for the interaction with FlhAC. These results suggest that FlgN efficiently transfers FlgK/L subunits to FlhAC to promote their export.

Bibliography

Minamino, T., Kinoshita, M., Hara, N., Takeuchi, S., Hida, A., Koya, S., Glenwright, H., Imada, K., Aldridge, P. D., & Namba, K. (2012). Interaction of a bacterial flagellar chaperone FlgN with FlhA is required for efficient export of its cognate substrates. Molecular Microbiology, 83(4), 775â788. Portico.

Authors 10

Tohru Minamino (first)
Miki Kinoshita (additional)
Noritaka Hara (additional)
Shiori Takeuchi (additional)
Akira Hida (additional)
Satomi Koya (additional)
Helen Glenwright (additional)
Katsumi Imada (additional)
Phillip D. Aldridge (additional)
Keiichi Namba (additional)

References 42 Referenced 78

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{'key': 'e_1_2_6_40_1', 'article-title': 'Functional defect and restoration of temperature‐sensitive mutants of FlhA, a subunit of the flagellar protein export apparatus', 'author': 'Shimada M.', 'year': '2011', 'journal-title': 'J Mol Biol'} / J Mol Biol / Functional defect and restoration of temperature‐sensitive mutants of FlhA, a subunit of the flagellar protein export apparatus by Shimada M. (2011)
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Dates

Type	When
Created	13 years, 7 months ago (Jan. 11, 2012, 4:38 a.m.)
Deposited	1 year, 10 months ago (Oct. 14, 2023, 3:32 p.m.)
Indexed	2 days, 20 hours ago (Aug. 21, 2025, 12:48 p.m.)
Issued	13 years, 7 months ago (Jan. 11, 2012)
Published	13 years, 7 months ago (Jan. 11, 2012)
Published Online	13 years, 7 months ago (Jan. 11, 2012)
Published Print	13 years, 6 months ago (Feb. 1, 2012)

Funders 0

None

BibTeX

@article{Minamino_2012, title={Interaction of a bacterial flagellar chaperone FlgN with FlhA is required for efficient export of its cognate substrates}, volume={83}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.2011.07964.x}, DOI={10.1111/j.1365-2958.2011.07964.x}, number={4}, journal={Molecular Microbiology}, publisher={Wiley}, author={Minamino, Tohru and Kinoshita, Miki and Hara, Noritaka and Takeuchi, Shiori and Hida, Akira and Koya, Satomi and Glenwright, Helen and Imada, Katsumi and Aldridge, Phillip D. and Namba, Keiichi}, year={2012}, month=jan, pages={775–788} }

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