DOI: 10.1111/j.1365-2958.2011.07924.x. Interaction between FliI ATPase and a flagellar chaperone FliT during bacterial flagellar protein export

Interaction between FliI ATPase and a flagellar chaperone FliT during bacterial flagellar protein export

10.1111/j.1365-2958.2011.07924.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryFliT is a flagellar type III export chaperone specific for the filament‐capping protein FliD. The FliT/FliD complex binds to the FliI ATPase of the flagellar export apparatus. The C‐terminal α4 helix of FliT controls its interaction with FliI but it remains unknown how it does so. Here, we analysed the FliI–FliT interaction by pull‐down assays using GST affinity chromatography. FliT94, missing the C‐terminal α4 helix, bound to the extreme N‐terminal region of FliI (FliIEN) with high affinity and to the C‐terminal ATPase domain (FliICAT) with low affinity. The C‐terminal α4 helix of FliT suppressed the interaction with FliIEN. FliH and FliT94 bound to a common binding site on FliIEN and hence FliH induced the release of FliI from FliT94 in an ATP‐independent manner. FliD increased the binding affinity of FliICAT for FliT. These results raise a possible hypothesis that the FliH/FliI complex binds to the FliT/FliD complex through FliICAT to escort it from the cytoplasm to the export gate made up of six integral membrane proteins and that, upon dissociation of FliD from FliT, FliT94 may bind to FliIEN and then FliI may transfer from FliT94 to FliH by the direct competition of FliT94 and FliH for FliIEN.

Bibliography

Minamino, T., Kinoshita, M., Imada, K., & Namba, K. (2011). Interaction between FliI ATPase and a flagellar chaperone FliT during bacterial flagellar protein export. Molecular Microbiology, 83(1), 168â178. Portico.

Authors 4

Tohru Minamino (first)
Miki Kinoshita (additional)
Katsumi Imada (additional)
Keiichi Namba (additional)

References 45 Referenced 48

Dates

Type	When
Created	13 years, 9 months ago (Nov. 23, 2011, 10:43 p.m.)
Deposited	1 year, 9 months ago (Oct. 30, 2023, 2:16 p.m.)
Indexed	3 months, 3 weeks ago (May 3, 2025, 1:07 p.m.)
Issued	13 years, 8 months ago (Dec. 7, 2011)
Published	13 years, 8 months ago (Dec. 7, 2011)
Published Online	13 years, 8 months ago (Dec. 7, 2011)
Published Print	13 years, 7 months ago (Jan. 1, 2012)

Funders 0

None

BibTeX

@article{Minamino_2011, title={Interaction between FliI ATPase and a flagellar chaperone FliT during bacterial flagellar protein export}, volume={83}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.2011.07924.x}, DOI={10.1111/j.1365-2958.2011.07924.x}, number={1}, journal={Molecular Microbiology}, publisher={Wiley}, author={Minamino, Tohru and Kinoshita, Miki and Imada, Katsumi and Namba, Keiichi}, year={2011}, month=dec, pages={168–178} }

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