DOI: 10.1111/j.1365-2958.2009.06613.x. Comparative cryo‐electron tomography of pathogenic Lyme disease spirochetes

Comparative cryo‐electron tomography of pathogenic Lyme disease spirochetes

10.1111/j.1365-2958.2009.06613.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummarySpirochetes of the Borrelia burgdorferi sensu lato group, the causative agents of Lyme borreliosis, exhibit a complex biology evolved in its zoonotic cycle. Cryo‐electron tomography was used to investigate structural features of three species, B. burgdorferi, B. garinii and B. afzelii, known to cause different clinical manifestations in humans. All three organisms revealed an overall similar architecture and showed different numbers of periplasmic flagellar filaments, polar periplasmic void regions, vesicles budding from the outer membrane sheath, which was covered by an amorphous slime layer. The latter was shown to be distinct in its density when comparing the three human‐pathogenic Lyme disease spirochetes and Borrelia hermsii, a species causing relapsing fever. Tomograms of dividing bacteria revealed vesicles near the site of division and new basal bodies that were attached at each end of newly establishing cytoplasmic cylinder poles, while periplasmic flagellar filaments still passed the impending site of division. Two different kinds of cytoplasmic filaments showed similarities to MreB or FtsZ filaments of other bacteria. The similar and distinct structural features of Borrelia and the previously investigated pathogenic and non‐pathogenic Treponema species emphasize the importance of further studying phylogenetically distant spirochetes.

Bibliography

Kudryashev, M., Cyrklaff, M., Baumeister, W., Simon, M. M., Wallich, R., & Frischknecht, F. (2009). Comparative cryoâelectron tomography of pathogenic Lyme disease spirochetes. Molecular Microbiology, 71(6), 1415â1434. Portico.

Authors 6

Mikhail Kudryashev (first)
Marek Cyrklaff (additional)
Wolfgang Baumeister (additional)
Markus M. Simon (additional)
Reinhard Wallich (additional)
Friedrich Frischknecht (additional)

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Dates

Type	When
Created	16 years, 6 months ago (Feb. 4, 2009, 4:59 a.m.)
Deposited	1 year, 10 months ago (Sept. 28, 2023, 3:16 p.m.)
Indexed	1 month, 1 week ago (July 14, 2025, 11:36 p.m.)
Issued	16 years, 5 months ago (March 1, 2009)
Published	16 years, 5 months ago (March 1, 2009)
Published Online	16 years, 5 months ago (March 13, 2009)
Published Print	16 years, 5 months ago (March 1, 2009)

Funders 0

None

BibTeX

@article{Kudryashev_2009, title={Comparative cryo‐electron tomography of pathogenic Lyme disease spirochetes}, volume={71}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.2009.06613.x}, DOI={10.1111/j.1365-2958.2009.06613.x}, number={6}, journal={Molecular Microbiology}, publisher={Wiley}, author={Kudryashev, Mikhail and Cyrklaff, Marek and Baumeister, Wolfgang and Simon, Markus M. and Wallich, Reinhard and Frischknecht, Friedrich}, year={2009}, month=mar, pages={1415–1434} }

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