DOI: 10.1111/j.1365-2958.2009.06601.x. Regulation of cell wall morphogenesis in <i>Bacillus subtilis</i> by recruitment of PBP1 to the MreB helix

Regulation of cell wall morphogenesis in Bacillus subtilis by recruitment of PBP1 to the MreB helix

10.1111/j.1365-2958.2009.06601.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryThe bacterial actin homologue MreB plays a key role in cell morphogenesis. In Bacillus subtilis MreB is essential under normal growth conditions and mreB mutants are defective in the control of cell diameter. However, the precise role of MreB is still unclear. Analysis of the lethal phenotypic consequences of mreB disruption revealed an unusual bulging phenotype that precedes cell death. A similar phenotype was seen in wild‐type cells at very low Mg2+ concentrations. We found that inactivation of the major bi‐functional penicillin‐binding protein (PBP) PBP1 of B. subtilis restored the viability of an mreB null mutant as well as preventing bulging in both mutant and wild‐type backgrounds. Bulging was associated with delocalization of PBP1. We show that the normal pattern of localization of PBP1 is dependent on MreB and that the proteins can physically interact using in vivo pull‐down and bacterial two‐hybrid approaches. Interactions between MreB and several other PBPs were also detected. Our results suggest that MreB filaments associate directly with the peptidoglycan biosynthetic machinery in B. subtilis as part of the mechanism that brings about controlled cell elongation.

Bibliography

Kawai, Y., Daniel, R. A., & Errington, J. (2009). Regulation of cell wall morphogenesis in Bacillus subtilis by recruitment of PBP1 to the MreB helix. Molecular Microbiology, 71(5), 1131â1144. Portico.

Authors 3

Yoshikazu Kawai (first)
Richard A. Daniel (additional)
Jeffery Errington (additional)

References 56 Referenced 121

10.1128/jb.177.3.765-773.1995
10.1046/j.1365-2958.2001.02631.x
10.1016/S1534-5807(02)00403-3
10.1016/j.devcel.2006.07.017
10.1111/j.1365-2958.2008.06210.x
10.1016/S0092-8674(03)00421-5
10.1128/JB.01031-06
10.1038/sj.embor.7400209
10.1111/j.1365-2958.2006.05457.x / Mol Microbiol / Dynamic localization and interaction with other Bacillus subtilis actin‐like proteins are important for the function of MreB by Defeu Soufo H.J. (2006)
10.1073/pnas.0507937102
10.1111/j.1365-2958.2007.05910.x
10.1073/pnas.0507708102
10.1038/35092500
10.1111/j.1365-2958.2006.05485.x
10.1111/j.1365-2958.2003.03936.x
10.1111/j.1365-2958.2005.04506.x
10.1101/gad.11.9.1160
10.1128/MMBR.62.4.1079-1093.1998
10.1128/MMBR.62.1.181-203.1998
10.1111/j.1365-2958.2007.05618.x
10.1046/j.1365-2958.2000.01928.x
10.1128/JB.00551-08
10.1111/j.1365-2958.2006.05184.x
10.1016/S0092-8674(01)00287-2
10.1073/pnas.95.10.5752
10.1093/emboj/cdg504
10.1111/j.1365-2958.2004.04367.x
10.1099/00221287-148-12-3971
10.1128/AEM.72.1.327-333.2006
10.1111/j.1365-2958.2005.04736.x
10.1016/S0378-1119(98)00580-0
10.1128/JB.185.4.1423-1431.2003
10.1111/j.1365-2958.2007.05851.x
10.1128/JB.180.17.4555-4563.1998
10.1128/JB.181.10.3201-3211.1999
10.1128/jb.175.15.4870-4876.1993
10.1128/jb.176.23.7197-7205.1994
10.1128/jb.178.7.2079-2085.1996
10.1073/pnas.0808352105
{'key': 'e_1_2_6_41_1', 'volume-title': 'Molecular Cloning: A Laboratory Manual', 'author': 'Sambrook J.', 'year': '1989'} / Molecular Cloning: A Laboratory Manual by Sambrook J. (1989)
10.1128/JB.186.15.5153-5156.2004
10.1073/pnas.96.25.14553
10.1126/science.1066274
10.1073/pnas.1232225100
10.1128/JB.187.1.54-64.2005
10.1016/j.cub.2003.10.024
10.1073/pnas.44.10.1072
10.1042/bj1130029
10.1073/pnas.0600829103
10.1099/00221287-144-11-3097
10.1128/JB.00455-07
10.1016/j.bbamem.2007.06.007
10.1111/j.1574-6976.2007.00094.x
10.1126/science.8160014
10.1093/emboj/16.8.2161
10.1128/AAC.43.5.1124

Dates

Type	When
Created	16 years, 7 months ago (Jan. 29, 2009, 5 a.m.)
Deposited	1 year, 11 months ago (Sept. 28, 2023, 2:49 p.m.)
Indexed	1 month, 3 weeks ago (July 11, 2025, 6:23 a.m.)
Issued	16 years, 6 months ago (Feb. 18, 2009)
Published	16 years, 6 months ago (Feb. 18, 2009)
Published Online	16 years, 6 months ago (Feb. 18, 2009)
Published Print	16 years, 6 months ago (March 1, 2009)

Funders 0

None

BibTeX

@article{Kawai_2009, title={Regulation of cell wall morphogenesis in Bacillus subtilis by recruitment of PBP1 to the MreB helix}, volume={71}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.2009.06601.x}, DOI={10.1111/j.1365-2958.2009.06601.x}, number={5}, journal={Molecular Microbiology}, publisher={Wiley}, author={Kawai, Yoshikazu and Daniel, Richard A. and Errington, Jeffery}, year={2009}, month=feb, pages={1131–1144} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        7,
        11
      ]
    ],
    "date-time": "2025-07-11T10:23:43Z",
    "timestamp": 1752229423300
  },
  "reference-count": 56,
  "publisher": "Wiley",
  "issue": "5",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2009,
            1,
            29
          ]
        ],
        "date-time": "2009-01-29T00:00:00Z",
        "timestamp": 1233187200000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2009,
        3
      ]
    ]
  },
  "abstract": "<jats:title>Summary</jats:title><jats:p>The bacterial actin homologue MreB plays a key role in cell morphogenesis. In <jats:italic>Bacillus subtilis</jats:italic> MreB is essential under normal growth conditions and <jats:italic>mreB</jats:italic> mutants are defective in the control of cell diameter. However, the precise role of MreB is still unclear. Analysis of the lethal phenotypic consequences of <jats:italic>mreB</jats:italic> disruption revealed an unusual bulging phenotype that precedes cell death. A similar phenotype was seen in wild\u2010type cells at very low Mg<jats:sup>2+</jats:sup> concentrations. We found that inactivation of the major bi\u2010functional penicillin\u2010binding protein (PBP) PBP1 of <jats:italic>B.\u2003subtilis</jats:italic> restored the viability of an <jats:italic>mreB</jats:italic> null mutant as well as preventing bulging in both mutant and wild\u2010type backgrounds. Bulging was associated with delocalization of PBP1. We show that the normal pattern of localization of PBP1 is dependent on MreB and that the proteins can physically interact using <jats:italic>in vivo</jats:italic> pull\u2010down and bacterial two\u2010hybrid approaches. Interactions between MreB and several other PBPs were also detected. Our results suggest that MreB filaments associate directly with the peptidoglycan biosynthetic machinery in <jats:italic>B.\u2003subtilis</jats:italic> as part of the mechanism that brings about controlled cell elongation.</jats:p>",
  "DOI": "10.1111/j.1365-2958.2009.06601.x",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2009,
        1,
        29
      ]
    ],
    "date-time": "2009-01-29T10:00:51Z",
    "timestamp": 1233223251000
  },
  "page": "1131-1144",
  "source": "Crossref",
  "is-referenced-by-count": 121,
  "title": "Regulation of cell wall morphogenesis in <i>Bacillus subtilis</i> by recruitment of PBP1 to the MreB helix",
  "prefix": "10.1111",
  "volume": "71",
  "author": [
    {
      "given": "Yoshikazu",
      "family": "Kawai",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Richard A.",
      "family": "Daniel",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Jeffery",
      "family": "Errington",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2009,
        2,
        18
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_6_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.177.3.765-773.1995"
    },
    {
      "key": "e_1_2_6_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2958.2001.02631.x"
    },
    {
      "key": "e_1_2_6_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S1534-5807(02)00403-3"
    },
    {
      "key": "e_1_2_6_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.devcel.2006.07.017"
    },
    {
      "key": "e_1_2_6_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.2008.06210.x"
    },
    {
      "key": "e_1_2_6_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(03)00421-5"
    },
    {
      "key": "e_1_2_6_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.01031-06"
    },
    {
      "key": "e_1_2_6_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/sj.embor.7400209"
    },
    {
      "key": "e_1_2_6_10_1",
      "doi-asserted-by": "crossref",
      "first-page": "1340",
      "DOI": "10.1111/j.1365-2958.2006.05457.x",
      "article-title": "Dynamic localization and interaction with other Bacillus subtilis actin\u2010like proteins are important for the function of MreB",
      "volume": "621",
      "author": "Defeu Soufo H.J.",
      "year": "2006",
      "journal-title": "Mol Microbiol"
    },
    {
      "key": "e_1_2_6_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0507937102"
    },
    {
      "key": "e_1_2_6_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.2007.05910.x"
    },
    {
      "key": "e_1_2_6_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0507708102"
    },
    {
      "key": "e_1_2_6_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/35092500"
    },
    {
      "key": "e_1_2_6_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.2006.05485.x"
    },
    {
      "key": "e_1_2_6_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.2003.03936.x"
    },
    {
      "key": "e_1_2_6_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.2005.04506.x"
    },
    {
      "key": "e_1_2_6_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1101/gad.11.9.1160"
    },
    {
      "key": "e_1_2_6_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MMBR.62.4.1079-1093.1998"
    },
    {
      "key": "e_1_2_6_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MMBR.62.1.181-203.1998"
    },
    {
      "key": "e_1_2_6_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.2007.05618.x"
    },
    {
      "key": "e_1_2_6_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1365-2958.2000.01928.x"
    },
    {
      "key": "e_1_2_6_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.00551-08"
    },
    {
      "key": "e_1_2_6_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.2006.05184.x"
    },
    {
      "key": "e_1_2_6_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0092-8674(01)00287-2"
    },
    {
      "key": "e_1_2_6_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.95.10.5752"
    },
    {
      "key": "e_1_2_6_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/cdg504"
    },
    {
      "key": "e_1_2_6_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.2004.04367.x"
    },
    {
      "key": "e_1_2_6_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1099/00221287-148-12-3971"
    },
    {
      "key": "e_1_2_6_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/AEM.72.1.327-333.2006"
    },
    {
      "key": "e_1_2_6_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.2005.04736.x"
    },
    {
      "key": "e_1_2_6_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0378-1119(98)00580-0"
    },
    {
      "key": "e_1_2_6_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.185.4.1423-1431.2003"
    },
    {
      "key": "e_1_2_6_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2958.2007.05851.x"
    },
    {
      "key": "e_1_2_6_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.180.17.4555-4563.1998"
    },
    {
      "key": "e_1_2_6_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.181.10.3201-3211.1999"
    },
    {
      "key": "e_1_2_6_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.175.15.4870-4876.1993"
    },
    {
      "key": "e_1_2_6_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.176.23.7197-7205.1994"
    },
    {
      "key": "e_1_2_6_39_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/jb.178.7.2079-2085.1996"
    },
    {
      "key": "e_1_2_6_40_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0808352105"
    },
    {
      "key": "e_1_2_6_41_1",
      "volume-title": "Molecular Cloning: A Laboratory Manual",
      "author": "Sambrook J.",
      "year": "1989"
    },
    {
      "key": "e_1_2_6_42_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.186.15.5153-5156.2004"
    },
    {
      "key": "e_1_2_6_43_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.96.25.14553"
    },
    {
      "key": "e_1_2_6_44_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.1066274"
    },
    {
      "key": "e_1_2_6_45_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.1232225100"
    },
    {
      "key": "e_1_2_6_46_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.187.1.54-64.2005"
    },
    {
      "key": "e_1_2_6_47_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.cub.2003.10.024"
    },
    {
      "key": "e_1_2_6_48_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.44.10.1072"
    },
    {
      "key": "e_1_2_6_49_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj1130029"
    },
    {
      "key": "e_1_2_6_50_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0600829103"
    },
    {
      "key": "e_1_2_6_51_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1099/00221287-144-11-3097"
    },
    {
      "key": "e_1_2_6_52_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/JB.00455-07"
    },
    {
      "key": "e_1_2_6_53_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.bbamem.2007.06.007"
    },
    {
      "key": "e_1_2_6_54_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1574-6976.2007.00094.x"
    },
    {
      "key": "e_1_2_6_55_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.8160014"
    },
    {
      "key": "e_1_2_6_56_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/emboj/16.8.2161"
    },
    {
      "key": "e_1_2_6_57_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/AAC.43.5.1124"
    }
  ],
  "container-title": "Molecular Microbiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2958.2009.06601.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2958.2009.06601.x",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        9,
        28
      ]
    ],
    "date-time": "2023-09-28T18:49:19Z",
    "timestamp": 1695926959000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2958.2009.06601.x"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2009,
        2,
        18
      ]
    ]
  },
  "references-count": 56,
  "journal-issue": {
    "issue": "5",
    "published-print": {
      "date-parts": [
        [
          2009,
          3
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1111/j.1365-2958.2009.06601.x"
  ],
  "URL": "http://dx.doi.org/10.1111/j.1365-2958.2009.06601.x",
  "relation": {},
  "ISSN": [
    "0950-382X",
    "1365-2958"
  ],
  "subject": [],
  "container-title-short": "Molecular Microbiology",
  "published": {
    "date-parts": [
      [
        2009,
        2,
        18
      ]
    ]
  }
}