DOI: 10.1111/j.1365-2958.2008.06219.x. Location and architecture of the <i>Caulobacter crescentus</i> chemoreceptor array

Location and architecture of the Caulobacter crescentus chemoreceptor array

10.1111/j.1365-2958.2008.06219.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryA new method for recording both fluorescence and cryo‐EM images of small bacterial cells was developed and used to identify chemoreceptor arrays in cryotomograms of intact Caulobacter crescentus cells. We show that in wild‐type cells preserved in a near‐native state, the chemoreceptors are hexagonally packed with a lattice spacing of 12 nm, just a few tens of nanometers away from the flagellar motor that they control. The arrays were always found on the convex side of the cell, further demonstrating that Caulobacter cells maintain dorsal/ventral as well as anterior/posterior asymmetry. Placing the known crystal structure of a trimer of receptor dimers at each vertex of the lattice accounts well for the density and agrees with other constraints. Based on this model for the arrangement of receptors, there are between one and two thousand receptors per array.

Bibliography

Briegel, A., Ding, H. J., Li, Z., Werner, J., Gitai, Z., Dias, D. P., Jensen, R. B., & Jensen, G. J. (2008). Location and architecture of the Caulobacter crescentus chemoreceptor array. Molecular Microbiology, 69(1), 30â41. Portico.

Authors 8

Ariane Briegel (first)
H. Jane Ding (additional)
Zhuo Li (additional)
John Werner (additional)
Zemer Gitai (additional)
D. Prabha Dias (additional)
Rasmus B. Jensen (additional)
Grant J. Jensen (additional)

References 60 Referenced 107

Dates

Type	When
Created	17 years, 5 months ago (March 20, 2008, 5:25 a.m.)
Deposited	1 year, 10 months ago (Sept. 28, 2023, 5:17 p.m.)
Indexed	5 months ago (March 19, 2025, 6:01 a.m.)
Issued	17 years, 1 month ago (June 28, 2008)
Published	17 years, 1 month ago (June 28, 2008)
Published Online	17 years, 1 month ago (June 28, 2008)
Published Print	17 years, 1 month ago (July 1, 2008)

Funders 0

None

BibTeX

@article{Briegel_2008, title={Location and architecture of the Caulobacter crescentus chemoreceptor array}, volume={69}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.2008.06219.x}, DOI={10.1111/j.1365-2958.2008.06219.x}, number={1}, journal={Molecular Microbiology}, publisher={Wiley}, author={Briegel, Ariane and Ding, H. Jane and Li, Zhuo and Werner, John and Gitai, Zemer and Dias, D. Prabha and Jensen, Rasmus B. and Jensen, Grant J.}, year={2008}, month=jun, pages={30–41} }

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