DOI: 10.1111/j.1365-2958.2007.06092.x. The <i>Streptomyces coelicolor</i> GlnR regulon: identification of new GlnR targets and evidence for a central role of GlnR in nitrogen metabolism in actinomycetes

The Streptomyces coelicolor GlnR regulon: identification of new GlnR targets and evidence for a central role of GlnR in nitrogen metabolism in actinomycetes

10.1111/j.1365-2958.2007.06092.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

Summary Streptomyces coelicolor GlnR is a global regulator that controls genes involved in nitrogen metabolism. By genomic screening 10 new GlnR targets were identified, including enzymes for ammonium assimilation (glnII, gdhA), nitrite reduction (nirB), urea cleavage (ureA) and a number of biochemically uncharacterized proteins (SCO0255, SCO0888, SCO2195, SCO2400, SCO2404, SCO7155). For the GlnR regulon, a GlnR binding site which comprises the sequence gTnAc‐n6‐GaAAc‐n6‐GtnAC‐n6‐GAAAc‐n6 has been found. Reverse transcription analysis of S. coelicolor and the S. coelicolor glnR mutant revealed that GlnR activates or represses the expression of its target genes. Furthermore, glnR expression itself was shown to be nitrogen‐dependent. Physiological studies of S. coelicolor and the S. coelicolor glnR mutant with ammonium and nitrate as the sole nitrogen source revealed that GlnR is not only involved in ammonium assimilation but also in ammonium supply. blast analysis demonstrated that GlnR‐homologous proteins are present in different actinomycetes containing the glnA gene with the conserved GlnR binding site. By DNA binding studies, it was furthermore demonstrated that S. coelicolor GlnR is able to interact with these glnA upstream regions. We therefore suggest that GlnR‐mediated regulation is not restricted to Streptomyces but constitutes a regulon conserved in many actinomycetes.

Bibliography

Tiffert, Y., Supra, P., Wurm, R., Wohlleben, W., Wagner, R., & Reuther, J. (2008). The Streptomyces coelicolor GlnR regulon: identification of new GlnR targets and evidence for a central role of GlnR in nitrogen metabolism in actinomycetes. Molecular Microbiology, 67(4), 861â880. Portico.

Authors 6

Yvonne Tiffert (first)
Petra Supra (additional)
Reinhild Wurm (additional)
Wolfgang Wohlleben (additional)
Rolf Wagner (additional)
Jens Reuther (additional)

References 57 Referenced 171

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Dates

Type	When
Created	15 years ago (Aug. 18, 2010, 7:14 a.m.)
Deposited	1 year, 11 months ago (Sept. 28, 2023, 7:42 p.m.)
Indexed	1 month ago (Aug. 6, 2025, 9:55 a.m.)
Issued	17 years, 8 months ago (Jan. 7, 2008)
Published	17 years, 8 months ago (Jan. 7, 2008)
Published Online	17 years, 8 months ago (Jan. 7, 2008)
Published Print	17 years, 7 months ago (Feb. 1, 2008)

Funders 0

None

BibTeX

@article{Tiffert_2008, title={The Streptomyces coelicolor GlnR regulon: identification of new GlnR targets and evidence for a central role of GlnR in nitrogen metabolism in actinomycetes}, volume={67}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.2007.06092.x}, DOI={10.1111/j.1365-2958.2007.06092.x}, number={4}, journal={Molecular Microbiology}, publisher={Wiley}, author={Tiffert, Yvonne and Supra, Petra and Wurm, Reinhild and Wohlleben, Wolfgang and Wagner, Rolf and Reuther, Jens}, year={2008}, month=jan, pages={861–880} }

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