DOI: 10.1111/j.1365-2958.2006.05250.x. Determinants of chemoreceptor cluster formation in <i>Escherichia coli</i>

Determinants of chemoreceptor cluster formation in Escherichia coli

10.1111/j.1365-2958.2006.05250.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryChemotactic stimuli in bacteria are sensed by large sensory complexes, or receptor clusters, that consist of tens of thousands of proteins. Receptor clusters appear to play a key role in signal processing, but their structure remains poorly understood. Here we used fluorescent protein fusions to study in vivo formation of the cluster core, which consists of receptors, a kinase CheA and an assisting protein CheW. We show that receptors aggregate through their cytoplasmic domains even in the absence of other chemotaxis proteins. Clustering is further enhanced by the binding of CheW. Surprisingly, we observed that some fragments of CheA bind receptor clusters well in the absence of CheW, although the latter does assist the binding of full‐length CheA. The resulting mode of receptor cluster formation is consistent with an experimentally observed flexible stoichiometry of chemosensory complexes and with assumptions of recently proposed computer models of signal processing in chemotaxis.

Bibliography

Kentner, D., Thiem, S., Hildenbeutel, M., & Sourjik, V. (2006). Determinants of chemoreceptor cluster formation in Escherichia coli. Molecular Microbiology, 61(2), 407â417. Portico.

Authors 4

David Kentner (first)
Sebastian Thiem (additional)
Markus Hildenbeutel (additional)
Victor Sourjik (additional)

References 47 Referenced 83

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Dates

Type	When
Created	19 years, 2 months ago (June 15, 2006, 7:30 a.m.)
Deposited	1 year, 10 months ago (Sept. 28, 2023, 12:25 p.m.)
Indexed	2 weeks, 2 days ago (Aug. 6, 2025, 8 a.m.)
Issued	19 years, 2 months ago (June 15, 2006)
Published	19 years, 2 months ago (June 15, 2006)
Published Online	19 years, 2 months ago (June 15, 2006)
Published Print	19 years, 1 month ago (July 1, 2006)

Funders 0

None

BibTeX

@article{Kentner_2006, title={Determinants of chemoreceptor cluster formation in Escherichia coli}, volume={61}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.2006.05250.x}, DOI={10.1111/j.1365-2958.2006.05250.x}, number={2}, journal={Molecular Microbiology}, publisher={Wiley}, author={Kentner, David and Thiem, Sebastian and Hildenbeutel, Markus and Sourjik, Victor}, year={2006}, month=jun, pages={407–417} }

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