Crossref journal-article
Wiley
Molecular Microbiology (311)
Abstract

SummaryType III secretion systems (TTSS) are used by many Gram‐negative pathogens for transporting effector proteins into eukaryotic host cells. Two modes of type III effector protein transport can be distinguished: transport into the surrounding medium (secretion) and cell‐contact induced injection of effector proteins directly into the host cell cytosol (translocation). Two domains within the N‐terminal regions of effector proteins determine the mode of transport. The amino terminal ∼20 amino acids (N‐terminal secretion signal, NSS) mediate secretion. In contrast, translocation generally requires the NSS, the adjacent ∼100 amino acids (chaperone binding domain, CBD) and binding of the cognate chaperone to this CBD. TTSS are phylogenetically related to flagellar systems. Because both systems are expressed in Salmonella Typhimurium, correct effector protein transport involves at least two decisions: transport via the Salmonella pathogenicity island 1 (SPI‐1) but not the flagellar TTSS (= specificity) and translocation into the host cell instead of secretion into the surrounding media (= transport mode). The mechanisms guiding these decisions are poorly understood. We have studied the S. Typhimurium effector protein SopE, which is specifically transported via the SPI‐1 TTSS. Secretion and translocation strictly require the cognate chaperone InvB. Alanine replacement of amino acids 30–42 (and to some extent 44–54) abolished tight InvB binding, abolished translocation into the host cell and led to secretion of SopE via both, the flagellar and the SPI‐1 TTSS. In clear contrast to wild‐type SopE, secretion of SopEAla30‐42 and SopEAla44‐54 via the SPI‐1 and the flagellar export system did not require InvB. These data reveal a novel function of the CBD: the CBD inhibits secretion of wild‐type SopE via the flagellar and the SPI‐1 TTSS in the absence of the chaperone InvB. Our data provide new insights into mechanisms ensuring specific effector protein transport by TTSS.

Bibliography

Ehrbar, K., Winnen, B., & Hardt, W. (2005). The chaperone binding domain of SopE inhibits transport via flagellar and SPI‐1 TTSS in the absence of InvB. Molecular Microbiology, 59(1), 248–264. Portico.

Authors 3
  1. Kristin Ehrbar (first)
  2. Brit Winnen (additional)
  3. Wolf‐Dietrich Hardt (additional)
References 48 Referenced 23
  1. 10.1126/science.278.5340.1140
  2. 10.1038/nsb1101-974
  3. 10.1128/JB.182.17.4811-4821.2000
  4. 10.1093/emboj/cdf329
  5. 10.1128/iai.64.9.3524-3531.1996
  6. 10.1093/emboj/20.8.1850
  7. 10.1128/JB.185.23.6950-6967.2003
  8. 10.1128/JB.186.4.1215-1219.2004
  9. 10.1016/S0378-1097(03)00042-9
  10. 10.1146/annurev.cellbio.17.1.53
  11. 10.1126/science.284.5418.1322
  12. 10.1073/pnas.86.16.6383
  13. 10.1128/MMBR.68.4.771-795.2004
  14. 10.1073/pnas.95.5.2574
  15. 10.1016/S0092-8674(00)81442-7
  16. 10.1046/j.1365-2958.2000.01935.x
  17. 10.1038/291238a0
  18. 10.1111/j.1365-2958.2005.04758.x
  19. 10.1111/j.1365-2958.1994.tb00450.x
  20. 10.1128/jb.177.24.7078-7085.1995
  21. 10.1111/j.1365-2958.1996.tb02571.x
  22. 10.1128/JB.185.24.7279-7284.2003
  23. 10.1046/j.1365-2958.2003.03840.x
  24. 10.1046/j.1365-2958.2001.02271.x
  25. 10.1128/IAI.58.1.137-143.1990
  26. 10.1038/nsb717
  27. 10.1128/JB.181.23.7149-7153.1999
  28. 10.1016/j.bbamcr.2004.04.005
  29. 10.1128/jb.170.6.2575-2583.1988
  30. 10.1073/pnas.96.17.9845
  31. 10.1128/JB.183.7.2348-2358.2001
  32. 10.1126/science.1107679
  33. 10.1128/iai.65.2.774-782.1997
  34. 10.1128/IAI.68.9.5050-5055.2000
  35. 10.1128/MCB.14.2.1333
  36. 10.1016/S1369-5274(02)00002-4
  37. 10.1002/j.1460-2075.1994.tb06341.x
  38. 10.1074/jbc.274.43.30501
  39. 10.1016/j.tim.2004.01.003
  40. {'key': 'e_1_2_7_41_1', 'volume-title': 'Molecular Cloning: A Laboratory Manual.', 'author': 'Sambrook J.', 'year': '1989'} / Molecular Cloning: A Laboratory Manual. by Sambrook J. (1989)
  41. 10.1073/pnas.0503407102
  42. 10.1128/iai.63.12.4600-4605.1995 / Infect Immun / Antigenic determinants of the OmpC porin from Salmonella typhimurium by Singh S.P. (1995)
  43. 10.1111/j.1365-2958.1994.tb02191.x
  44. 10.1038/35102073
  45. 10.1128/IAI.72.7.4138-4150.2004
  46. 10.1046/j.1365-2958.1996.00116.x
  47. 10.1016/S1286-4579(01)01489-7
  48. 10.1128/IAI.63.10.4024-4028.1995
Dates
Type When
Created 19 years, 10 months ago (Oct. 27, 2005, 7:08 a.m.)
Deposited 1 year, 11 months ago (Sept. 28, 2023, 3:23 p.m.)
Indexed 1 year, 7 months ago (Jan. 19, 2024, 4:14 p.m.)
Issued 19 years, 9 months ago (Nov. 14, 2005)
Published 19 years, 9 months ago (Nov. 14, 2005)
Published Online 19 years, 9 months ago (Nov. 14, 2005)
Published Print 19 years, 7 months ago (Jan. 1, 2006)
Funders 0

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@article{Ehrbar_2005, title={The chaperone binding domain of SopE inhibits transport via flagellar and SPI‐1 TTSS in the absence of InvB}, volume={59}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.2005.04931.x}, DOI={10.1111/j.1365-2958.2005.04931.x}, number={1}, journal={Molecular Microbiology}, publisher={Wiley}, author={Ehrbar, Kristin and Winnen, Brit and Hardt, Wolf‐Dietrich}, year={2005}, month=nov, pages={248–264} }