DOI: 10.1111/j.1365-2958.2005.04887.x. The ExPortal: an organelle dedicated to the biogenesis of secreted proteins in <i>Streptococcus pyogenes</i>

The ExPortal: an organelle dedicated to the biogenesis of secreted proteins in Streptococcus pyogenes

10.1111/j.1365-2958.2005.04887.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryThe Gram‐positive pathogen Streptococcus pyogenes secretes proteins through the ExPortal, a unique single microdomain of the cellular membrane specialized to contain the Sec translocons. It has been proposed that the ExPortal functions as an organelle to promote the biogenesis of secreted proteins by coordinating interactions between nascent unfolded secretory proteins and membrane‐associated chaperones. In this study we provide evidence to support this model. It was found that HtrA (DegP), a surface anchored accessory factor required for maturation of the secreted SpeB cysteine protease, was localized exclusively to the ExPortal. Furthermore, the ATP synthase β subunit was not localized to the ExPortal, suggesting that retention is likely restricted to a specific subset of exported proteins. Mutations that disrupted the anchoring, but not the protease activity, of HtrA, also altered the maturation kinetics of SpeB demonstrating that localization to the ExPortal was important for HtrA function. These data indicate that the ExPortal provides a mechanism by which Gram‐positive bacteria can coordinate protein secretion and subsequent biogenesis in the absence of a specialized protein‐folding compartment.

Bibliography

Rosch, J. W., & Caparon, M. G. (2005). The ExPortal: an organelle dedicated to the biogenesis of secreted proteins in Streptococcus pyogenes. Molecular Microbiology, 58(4), 959â968. Portico.

Authors 2

Jason W. Rosch (first)
Michael G. Caparon (additional)

References 26 Referenced 85

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{'key': 'e_1_2_6_23_1', 'first-page': '311', 'article-title': 'Post‐translocational folding of secretory proteins in Gram‐positive bacteria', 'volume': '1693', 'author': 'Sarvas M.', 'year': '2004', 'journal-title': 'Biochim Biophys Acta'} / Biochim Biophys Acta / Post‐translocational folding of secretory proteins in Gram‐positive bacteria by Sarvas M. (2004)
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Dates

Type	When
Created	19 years, 11 months ago (Sept. 29, 2005, 5:44 a.m.)
Deposited	1 year, 11 months ago (Sept. 27, 2023, 10:08 a.m.)
Indexed	1 year, 1 month ago (July 16, 2024, 7:31 p.m.)
Issued	19 years, 10 months ago (Oct. 14, 2005)
Published	19 years, 10 months ago (Oct. 14, 2005)
Published Online	19 years, 10 months ago (Oct. 14, 2005)
Published Print	19 years, 10 months ago (Nov. 1, 2005)

Funders 0

None

BibTeX

@article{Rosch_2005, title={The ExPortal: an organelle dedicated to the biogenesis of secreted proteins in Streptococcus pyogenes}, volume={58}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.2005.04887.x}, DOI={10.1111/j.1365-2958.2005.04887.x}, number={4}, journal={Molecular Microbiology}, publisher={Wiley}, author={Rosch, Jason W. and Caparon, Michael G.}, year={2005}, month=oct, pages={959–968} }

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