DOI: 10.1111/j.1365-2958.2004.04375.x. Rpc25, a conserved RNA polymerase III subunit, is critical for transcription initiation

Rpc25, a conserved RNA polymerase III subunit, is critical for transcription initiation

10.1111/j.1365-2958.2004.04375.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryRpc25 is a strongly conserved subunit of RNA polymerase III with homology to Rpa43 in RNA polymerase I, Rpb7 in RNA polymerase II and the archaeal RpoE subunit. A central domain of Rpc25 can replaced the corresponding region of Rpb7 with little or no growth defect, underscoring the functional relatedness of these proteins. Rpc25 forms a heterodimer with Rpc17, another conserved component of RNA polymerase III. A conditional mutant (rpc25‐S100P) impairs this interaction. rpc25‐S100P and another conditional mutant obtained by complementation with the Schizosaccharomyces pombe subunit (rpc25‐Sp) were investigated for the properties of their purified RNA polymerase III. The mutant enzymes were defective in the specific synthesis of pre‐tRNA transcripts but acted at a wild‐type level on poly[d(A‐T)] templates. They were also indistinguishable from wild type in transcript elongation, cleavage and termination. These data indicate that Rpc25 is needed for transcription initiation but is not critical for the elongating properties of RNA polymerase III.

Bibliography

Zaros, C., & Thuriaux, P. (2004). Rpc25, a conserved RNA polymerase III subunit, is critical for transcription initiation. Molecular Microbiology, 55(1), 104â114. Portico.

Authors 2

Cécile Zaros (first)
Pierre Thuriaux (additional)

References 51 Referenced 28

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Dates

Type	When
Created	20 years, 8 months ago (Dec. 21, 2004, 6:51 p.m.)
Deposited	1 year, 10 months ago (Oct. 14, 2023, 6:57 a.m.)
Indexed	4 months, 1 week ago (April 10, 2025, 7:10 a.m.)
Issued	20 years, 8 months ago (Dec. 2, 2004)
Published	20 years, 8 months ago (Dec. 2, 2004)
Published Online	20 years, 8 months ago (Dec. 2, 2004)
Published Print	20 years, 7 months ago (Jan. 1, 2005)

Funders 0

None

BibTeX

@article{Zaros_2004, title={Rpc25, a conserved RNA polymerase III subunit, is critical for transcription initiation}, volume={55}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.2004.04375.x}, DOI={10.1111/j.1365-2958.2004.04375.x}, number={1}, journal={Molecular Microbiology}, publisher={Wiley}, author={Zaros, Cécile and Thuriaux, Pierre}, year={2004}, month=dec, pages={104–114} }

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