DOI: 10.1111/j.1365-2958.2003.03972.x. Zinc is a key factor in controlling alternation of two types of L31 protein in the<i>Bacillus subtilis</i>ribosome

Zinc is a key factor in controlling alternation of two types of L31 protein in theBacillus subtilisribosome

10.1111/j.1365-2958.2003.03972.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryWe have analysed changes in the composition of ribosomal proteins during cell growth inBacillus subtilis. Ribosome fractions were prepared fromB. subtiliscells at different phases of growth and were separated by radical‐free and highly reducing (RFHR) two‐dimensional polyacrylamide gel electrophoresis. We identified 50 ribosomal proteins, including two paralogues of L31 protein (RpmE and YtiA). Although the ribosome fraction extracted from exponentially growing cells contained RpmE protein, this protein disappeared during the stationary phase. In contrast, YtiA was detected in the ribosome fraction extracted after the end of exponential growth. Expression of theytiAgene encoding YtiA was found to be negatively controlled by Zur, a zinc‐specific transcriptional repressor that controls zinc transport operons. Analysis by inductively coupled plasma mass spectrometry (ICP‐MS) indicated that RpmE contains one zinc ion per molecule of protein. In addition, mutagenesis of therpmEgene encoding RpmE revealed that Cys‐36 and Cys‐39, located within a CxxC motif, are required not only for binding zinc but also for the accumulation of RpmE in the cell. Taken together, these results indicate that zinc plays an essential role in the alternation between two types of L31 protein in the ribosome ofB. subtilis.

Bibliography

Nanamiya, H., Akanuma, G., Natori, Y., Murayama, R., Kosono, S., Kudo, T., Kobayashi, K., Ogasawara, N., Park, S., Ochi, K., & Kawamura, F. (2004). Zinc is a key factor in controlling alternation of two types of L31 protein in theBacillus subtilisribosome. Molecular Microbiology, 52(1), 273â283. Portico.

Authors 11

Hideaki Nanamiya (first)
Genki Akanuma (additional)
Yousuke Natori (additional)
Rikinori Murayama (additional)
Saori Kosono (additional)
Toshiaki Kudo (additional)
Kazuo Kobayashi (additional)
Naotake Ogasawara (additional)
Seung‐Moon Park (additional)
Kozo Ochi (additional)
Fujio Kawamura (additional)

References 33 Referenced 111

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Dates

Type	When
Created	20 years, 8 months ago (Dec. 27, 2004, 6 p.m.)
Deposited	8 months ago (Dec. 21, 2024, 2:34 p.m.)
Indexed	1 month ago (July 26, 2025, 5:25 a.m.)
Issued	21 years, 4 months ago (April 1, 2004)
Published	21 years, 4 months ago (April 1, 2004)
Published Online	16 years, 11 months ago (Sept. 22, 2008)
Published Print	21 years, 4 months ago (April 1, 2004)

Funders 0

None

BibTeX

@article{Nanamiya_2004, title={Zinc is a key factor in controlling alternation of two types of L31 protein in theBacillus subtilisribosome}, volume={52}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.2003.03972.x}, DOI={10.1111/j.1365-2958.2003.03972.x}, number={1}, journal={Molecular Microbiology}, publisher={Wiley}, author={Nanamiya, Hideaki and Akanuma, Genki and Natori, Yousuke and Murayama, Rikinori and Kosono, Saori and Kudo, Toshiaki and Kobayashi, Kazuo and Ogasawara, Naotake and Park, Seung‐Moon and Ochi, Kozo and Kawamura, Fujio}, year={2004}, month=apr, pages={273–283} }

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