DOI: 10.1111/j.1365-2958.1996.tb02485.x. Molecular biology of iron acquisition in <i>Saccharomyces cerevisiae</i>

Molecular biology of iron acquisition in Saccharomyces cerevisiae

10.1111/j.1365-2958.1996.tb02485.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryIn recent years, significant advances have been made in our understanding of the mechanism and regulation of elemental iron transport in the eukaryote Saccharomyces cerevisiae. This organism employs two distinct iron‐transport systems, depending on the bioavailability of the metal. In iron‐replete environments, a low‐affinity transport system (Km=30μM) is used to acquire iron. This system may also be used to acquire other metals including cobalt and cadmium. When environmental iron is limiting, a high‐affinity (Km=0.15 μM) iron‐transport system is induced. Genetic studies in S. cerevisiae have identified multiple genes involved in both iron‐transport systems. Cell‐surface reductases, FRE1 and FRE2, provide ferrous iron for both systems. A non‐ATP‐dependent transmembrane transporter (FET4) has been identified as the main component of low‐affinity transport. One gene identified to date as part of the high‐affinity transport system is FET3, which shows high sequence and functional homology to multicopper oxidases. Accessory genes required for the functioning of this transport system include a plasma‐membrane copper transporter (CTR1), an intracellular copper transporter (CCC2), and a putative transcription factor (AFT1). The mechanism by which these genes act in concert to ensure iron accumulation in S. cerevisiae presents an intriguing picture, drawing parallels with observations made in the human system almost 40 years ago.

Bibliography

Askwith, C. C., de Silva, D., & Kaplan, J. (1996). Molecular biology of iron acquisition in Saccharomyces cerevisiae. Molecular Microbiology, 20(1), 27â34. Portico.

Authors 3

Candice C. Askwith (first)
Deepika de Silva (additional)
Jerry Kaplan (additional)

References 54 Referenced 100

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 6, 2006, 6:58 a.m.)
Deposited	1 year, 11 months ago (Sept. 27, 2023, 3:01 p.m.)
Indexed	2 months, 3 weeks ago (June 10, 2025, 1:48 p.m.)
Issued	29 years, 5 months ago (April 1, 1996)
Published	29 years, 5 months ago (April 1, 1996)
Published Online	18 years, 10 months ago (Oct. 6, 2006)
Published Print	29 years, 5 months ago (April 1, 1996)

Funders 0

None

BibTeX

@article{Askwith_1996, title={Molecular biology of iron acquisition in Saccharomyces cerevisiae}, volume={20}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1996.tb02485.x}, DOI={10.1111/j.1365-2958.1996.tb02485.x}, number={1}, journal={Molecular Microbiology}, publisher={Wiley}, author={Askwith, Candice C. and de Silva, Deepika and Kaplan, Jerry}, year={1996}, month=apr, pages={27–34} }

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