DOI: 10.1111/j.1365-2958.1995.tb02437.x. Cloning and expression of a murein hydrolase lipoprotein from <i>Escherichia coli</i>

Cloning and expression of a murein hydrolase lipoprotein from Escherichia coli

10.1111/j.1365-2958.1995.tb02437.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryOn the basis of the published N‐terminal amino acid sequence of the soluble lytic transglycosylase 35 (Slt35) of Escherichia coli, an open reading frame (ORF) was cloned from the 60.8 min region of the E. coli chromosome. The nucleotide sequence of the ORF, containing a putative lipoprotein‐processing site, was shown by [3H]‐palmitate labelling to encode a lipoprotein with an apparent molecular mass of 36 kDa. A larger protein, presumably the prolipoprotein form, accumulated in the presence of globomycin. Over‐expression of the gene, designated mltB (for membrane‐bound lytic transglycosylase B), caused a 55‐fold increase in murein hydrolase activity in the membrane fraction and resulted in rapid cell lysis. After membrane fractionation by sucrose‐density‐gradient centrifugation, most of the induced enzyme activity was present in the outer and intermediate membrane fractions. Murein hydrolase activity in the soluble fraction of a homogenate of cells induced for MltB increased with time. This release of enzyme activity into the supernatant could be inhibited by the addition of the serine‐protease inhibitor phenylmethyl‐sulphonyl fluoride. It is concluded that the previously isolated Slt35 protein is a proteolytic degradation product of the murein hydrolase lipoprotein MltB. Surprisingly, a deletion in the mltB gene showed no obvious phenotype.

Bibliography

Ehlert, K., HÃ¶itje, J., & Templin, M. F. (1995). Cloning and expression of a murein hydrolase lipoprotein from Escherichia coli. Molecular Microbiology, 16(4), 761â768. Portico.

Authors 3

Kerstin Ehlert (first)
Joachim‐Volker Höitje (additional)
Markus F. Templin (additional)

References 47 Referenced 55

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Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 11:20 p.m.)
Deposited	1 year, 10 months ago (Oct. 26, 2023, 1:38 p.m.)
Indexed	1 year, 7 months ago (Jan. 18, 2024, 9:26 a.m.)
Issued	30 years, 4 months ago (May 1, 1995)
Published	30 years, 4 months ago (May 1, 1995)
Published Online	18 years, 10 months ago (Oct. 27, 2006)
Published Print	30 years, 4 months ago (May 1, 1995)

Funders 0

None

BibTeX

@article{Ehlert_1995, title={Cloning and expression of a murein hydrolase lipoprotein from Escherichia coli}, volume={16}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1995.tb02437.x}, DOI={10.1111/j.1365-2958.1995.tb02437.x}, number={4}, journal={Molecular Microbiology}, publisher={Wiley}, author={Ehlert, Kerstin and Höitje, Joachim‐Volker and Templin, Markus F.}, year={1995}, month=may, pages={761–768} }

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      ]
    ]
  }
}