DOI: 10.1111/j.1365-2958.1995.tb02387.x. Complementation studies with the gas vesicle‐encoding p‐vac region of <i>Halobacterium salinarium</i> PHH1 reveal a regulatory role for the p‐<i>gvpDE</i> genes

Complementation studies with the gas vesicle‐encoding p‐vac region of Halobacterium salinarium PHH1 reveal a regulatory role for the p‐gvpDE genes

10.1111/j.1365-2958.1995.tb02387.x

Crossref journal-article

Wiley

Molecular Microbiology (311)

Abstract

SummaryGas‐vesicle (Vac) synthesis in Halobacterium salinarium PHH1 involves the expression of the p‐vac region consisting of 14 different gvp genes that are arranged in two clusters: p‐gvpACNO and, oppositely oriented, p‐gvpDEFGHIJKLM. The latter cluster of genes is transcribed as two units: p‐gvpDE and p‐gvpF–M. The 5′‐terminus of the p‐gvpF–M mRMA was located 169 nucleotides upstream of p‐gvpF within p‐gvpE. The p‐gvpG and p‐gvpK gene was expressed in Escherichia coli and antibodies to proteins obtained were raised in rabbits. Both proteins could be detected in halobacterial cell lysates; in gas‐vesicle preparations, however, neither GvpG nor GvpK could be found. The requirement for single p‐gvp gene expression for gas‐vesicle synthesis was determined by transformation experiments using the Vac− species Haloferax volcanii as recipient. Construct ΔA containing all p‐gvp genes except for p‐gvpA, encoding the major gas‐vesicle structural protein, produced Vac− transformants, but the addition of p‐gvpA on a second vector restored gas‐vesicle synthesis to wild‐type level (Vac++). Similarly, double transformants containing p‐gvpD–M plus p‐gvpACNO, or p‐gvpG–M (fused to the promoter of the halobacterial ferredoxin gene for expression) plus p‐gvpFED–ACNO were Vac++. Transformants containing the p‐vac region either lacking gvpA, gvpF, or gvpGHI were Vac−, indicating the absolute requirement of these gvp genes (or at least one in the case of gvpGHI) for gas‐vesicle formation. Double transformants containing the constructs p‐gvpF–M plus p‐gvpACNO (ΔDE) accumulated gas vesicles (Vac+) but synthesized fewer than the wild type, showing that the p‐gvpDE genes are not necessary for gas‐vesicle assembly. A repressor function affecting the synthesis of the p‐gvpF–M mRNA could be suggested for p‐gvpD and the 5′‐ region of its mRNA.

Bibliography

Offner, S., & Pfeifer, F. (1995). Complementation studies with the gas vesicleâencoding pâvac region of Halobacterium salinarium PHH1 reveal a regulatory role for the pâgvpDE genes. Molecular Microbiology, 16(1), 9â19. Portico.

Authors 2

Sonja Offner (first)
Felicitas Pfeifer (additional)

References 33 Referenced 41

{'key': 'e_1_2_1_2_1', 'volume-title': 'Current Protocols in Molecular Biology', 'author': 'Ausubel F.', 'year': '1989'} / Current Protocols in Molecular Biology by Ausubel F. (1989)
10.1073/pnas.87.17.6772
{'key': 'e_1_2_1_4_1', 'first-page': '156', 'article-title': 'Single step method of RNA isolation by acid guanidinium thiocyanate‐phenol‐chloroform extraction', 'volume': '162', 'author': 'Chomozynski P.', 'year': '1987', 'journal-title': 'Anal Biochem'} / Anal Biochem / Single step method of RNA isolation by acid guanidinium thiocyanate‐phenol‐chloroform extraction by Chomozynski P. (1987)
Englert C.(1992)Struktur und Synthese der Gasvesikel des halophilen ArchaeonsHaloferax mediterranel. Dissertation LMU München.
10.1016/S0021-9258(18)98354-7 / J Biol Chem / Analysis of gas‐vesicle gene expression in Haloferax mediterranei reveals that GvpA and GvpC are both gas‐vesicle structural proteins by Englert C (1993)
10.1007/BF00633822
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10.1128/jb.175.3.684-692.1993 / J Bacteriol / The rightward gas‐vesicle operon in Halobacterium plasmid pNRC100: identification of the gvpA and gvpC gene products by use of antibody probes and genetic analysis of the region downstream of gvpC by Halladay J. (1993)
10.1007/BF00245155
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10.1128/JB.170.8.3718-3724.1988
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10.1128/mr.58.1.94-144.1994

Dates

Type	When
Created	18 years, 10 months ago (Oct. 27, 2006, 11:08 p.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 1:37 p.m.)
Indexed	1 year, 1 month ago (Aug. 5, 2024, 7:52 p.m.)
Issued	30 years, 5 months ago (April 1, 1995)
Published	30 years, 5 months ago (April 1, 1995)
Published Online	18 years, 10 months ago (Oct. 27, 2006)
Published Print	30 years, 5 months ago (April 1, 1995)

Funders 0

None

BibTeX

@article{Offner_1995, title={Complementation studies with the gas vesicle‐encoding p‐vac region of Halobacterium salinarium PHH1 reveal a regulatory role for the p‐gvpDE genes}, volume={16}, ISSN={1365-2958}, url={http://dx.doi.org/10.1111/j.1365-2958.1995.tb02387.x}, DOI={10.1111/j.1365-2958.1995.tb02387.x}, number={1}, journal={Molecular Microbiology}, publisher={Wiley}, author={Offner, Sonja and Pfeifer, Felicitas}, year={1995}, month=apr, pages={9–19} }

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